DNA Gyrase and Topoisomerase IV Mutations and their effect on Quinolones Resistant Proteus mirabilis among UTIs Patients

Abdelkreem, Randa Hassan; Yousuf, Amjad; Elmekki, Miskelyemen A.; Elhassan, Mogahid M.

doi:10.12669/pjms.36.6.2207

Cited by 8 publications

(7 citation statements)

References 22 publications

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“…Therefore, drug susceptibility testing should be conducted for all patients with comparable infections before starting a specific medicine. 24 The mutations in target enzymes (GyrB (Ser-464) and ParC (Ser-80) codons) and AcrAB efflux pump were investigated in relation to P. mirabilis resistance against fluoroquinolones. However, any relationship between mutation numbers in ParC, GyrA, and GyrB genes and the degree of P. mirabilis resistance to fluoroquinolone was not observed.…”

Section: Resistance To Fluoroquinolonesmentioning

confidence: 99%

Antibiotic Resistance in Proteus mirabilis: Mechanism, Status, and Public Health Significance

Alqurashi¹,

Elbanna²,

Ahmad³

et al. 2022

J. Pure Appl. Microbiol.

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Proteus mirabilis is a specific opportunistic pathogen of many infections including urinary tract infections (UTIs). Risk factors are linked with the acquisition of multidrug-resistant (MDR) to 3 or more classes of antimicrobials) strains. The resistance in extended-spectrum alpha-lactamase is rare, but the rising resistance in extended-spectrum beta-lactamase (ESBL) producing strains is a matter of concern. β-lactamases and antibiotic modifying enzymes mainly constitute the ESBLs resistance mechanism by hydrolyzing the antibiotics. Mutation or Porin loss could lead to the reduced permeability of antibiotics, enhanced efflux pump activity hindering the antibiotic access to the target site, antibiotic failure to bind at the target site because of the target modification, and lipopolysaccharide mutation causing the resistance against polymyxin antibiotics. This review aimed to explore various antimicrobial resistance mechanisms in Proteus mirabilis and their impact on public health status.

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Section: Resistance To Fluoroquinolonesmentioning

confidence: 99%

Antibiotic Resistance in Proteus mirabilis: Mechanism, Status, and Public Health Significance

Alqurashi¹,

Elbanna²,

Ahmad³

et al. 2022

J. Pure Appl. Microbiol.

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“…The dried DNA pellet was dissolved in 30 ul of 8 mM NaOH and stored at -20°C. 8 Phenol-Chloroform Method: DNA was also extracted with phenol-chloroform method, described by Kochl et al 9 The extracted DNA was immediately evaluated for quantity, purity and integrity. Results obtained were compared with the present TRIzol TM optimized method.…”

Section: Rna and Dna Co-extraction Procedurementioning

confidence: 99%

Two hours method for RNA and DNA co-extraction from blood of coronary artery disease patients: Fast, simple and economical technique

et al. 2022

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Objectives: Extraction of DNA and RNA is the first step in genomics and transcriptomics studies. Phenol-chloroform method for DNA extraction has been the widely used method. However, this method is relatively expensive and time-consuming. The objective of the present study was to validate a cost and time-effective protocol that will reduce the burden of molecular biology-based research and make a difference in laboratories with limited resources. Methods: A comparative study was conducted at Syed Qamer Alam Research Laboratory, Shifa College of Medicine; from February, 2021 to August, 2021. TRIzolTM method was used to extract RNA from blood samples of coronary artery disease patients and remnant was used to extract DNA. The quantity, purity and integrity of the extracted DNA by both methods (TRIzol and phenol-chloroform) was examined. PCR product amplification was performed with thrombomodulin (THBD) gene to validate the characteristic of the extracted DNA and its efficiency for downstream experiments. Results: The DNA yield in the TRIzolTM method was three-fold higher than phenol chloroform method. Both methods showed intact genomic DNA on the agarose gel, and extracted DNA was efficient for PCR amplification. Conclusion: The TRIzolTM method for RNA and DNA co-extraction is fast, simple and economical technique. So, it can be adopted for routine molecular biology analyses in limited resources setup. doi: https://doi.org/10.12669/pjms.38.7.5509 How to cite this:Dandare A, Rafiq M, Liaquat A, Khan MJ. Two hours method for RNA and DNA co-extraction from blood of coronary artery disease patients: Fast, simple and economical technique. Pak J Med Sci. 2022;38(7):---------. doi: https://doi.org/10.12669/pjms.38.7.5509 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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“…The mutations in the DNA gyrase binding site residues lead to Bacterial resistance. [6][7][8] The activities of the different generations of FQs are mainly dependent on the substitutions at the N 1 , C 8, and substitutions on the piperazine NÀ H. The beta keto acid at the third position is essential for binding the FQ with the metal and intercalating in the DNA strand. The ethyl and cyclopropyl substitutions at N1, as in norfloxacin (I), lomefloxacin (II), ciprofloxacin (III), moxifloxacin (IV), led to improved activity.…”

Section: Introductionmentioning

confidence: 99%

“…They inhibit DNA replication and cell growth/division. The mutations in the DNA gyrase binding site residues lead to Bacterial resistance [6–8] …”

Section: Introductionmentioning

confidence: 99%

Synthesis and Biological Evaluation of Diphenyl Ether‐Linked Quinolone Derivatives as Antibacterial Agents

Ghouse,

Akhir,

Sinha

et al. 2023

ChemistrySelect

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The increase in the prevalence of multi‐drug‐resistant bacteria poses a significant healthcare challenge. The urgent need to combat the resistant microbes necessitates discovering new antibacterial agents capable of circumventing the existing resistance mechanisms. Targeting DNA gyrase by suitably modifying the fluoroquinolones can lead to antibiotics with better activity and lower incidence of resistance. The substituted diphenyl ethers like triclosan are known to have potent antibacterial activity. In the current study, the hybridisation of diphenyl ether moiety with fluoroquinolones led to the design and synthesis of new compounds with potent inhibitory activity against staphylococcus aureus with MIC 0.5–64 μg/mL and moderately active against mycobacteria with MIC 2–64 μg/mL. The compounds are non‐toxic to Vero cells with a selectivity index >10 to 200. The compounds also inhibited the resistant strains of S. aureus with a MIC ranging from 0.5–64 μg/mL. The synthesised compounds also exhibited potent anti‐biofilm activity against S. aureus. Furthermore, the DNA supercoiling assay revealed the compounds 7 i, 7 o, 7 p and 7 q showed concentration‐dependant DNA‐Gyrase inhibition at 1 μg/mL.

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DNA Gyrase and Topoisomerase IV Mutations and their effect on Quinolones Resistant Proteus mirabilis among UTIs Patients

Cited by 8 publications

References 22 publications

Antibiotic Resistance in Proteus mirabilis: Mechanism, Status, and Public Health Significance

Antibiotic Resistance in Proteus mirabilis: Mechanism, Status, and Public Health Significance

Two hours method for RNA and DNA co-extraction from blood of coronary artery disease patients: Fast, simple and economical technique

Synthesis and Biological Evaluation of Diphenyl Ether‐Linked Quinolone Derivatives as Antibacterial Agents

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