DNA groove preference shift upon phosphorylation of a protamine-like cationic peptide

Chhetri, Khadka B.; Jang, Yun Hee; Lansac, Yves; Maiti, Prabal K.

doi:10.1039/d3cp03803c

Phys. Chem. Chem. Phys.

2023

DOI: 10.1039/d3cp03803c

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DNA groove preference shift upon phosphorylation of a protamine-like cationic peptide

Khadka B. Chhetri,

Yun Hee Jang,

Yves Lansac

et al.

Abstract: Entropic and Enthalpic contributions to Gibbs free energy of binding in four DNA-peptide complexes. It is observed that the binding of a protamine-like cationic peptide to DNA is enthalpy-driven for both major and minor grooves of DNA.

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2024

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The protamines of the noble false widow spiderSteatoda nobilisprovide an example of liquid-liquid phase separation chromatin transitions during spermiogenesis

Leyden,

Michalik,

Baruffaldi

et al. 2024

Preprint

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While there is extensive information about sperm nuclear basic proteins (SNBP) in vertebrates, there is very little information about Arthropoda by comparison. This paper aims to contribute to filling this gap by analyzing these proteins in the sperm of the noble false widow spiderSteatoda nobilis(Order Araneae, Family Theridiidae). To this end, we have developed a protein extraction method that allows the extraction of cysteine-containing protamines suitable for the preparation and analysis of SNBPs from samples where the amount of starting tissue material is limited. We carried out top-down mass spectrometry sequencing and molecular phylogenetic analyses to characterize the protamines ofS. nobilisand other spiders. We also used electron microscopy to analyze the chromatin organization of the sperm, and we found it to exhibit liquid-liquid phase spinodal decomposition during the late stages of spermiogenesis. These studies further our knowledge of the distribution of SNBPs within the animal kingdom and provide additional support for a proposed evolutionary origin of many protamines from a histone H1 (H5) replication-independent precursor.

show abstract

The protamines of the noble false widow spiderSteatoda nobilisprovide an example of liquid-liquid phase separation chromatin transitions during spermiogenesis

Leyden,

Michalik,

Baruffaldi

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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DNA groove preference shift upon phosphorylation of a protamine-like cationic peptide

Abstract: Entropic and Enthalpic contributions to Gibbs free energy of binding in four DNA-peptide complexes. It is observed that the binding of a protamine-like cationic peptide to DNA is enthalpy-driven for both major and minor grooves of DNA.

Cited by 1 publication

References 72 publications

The protamines of the noble false widow spiderSteatoda nobilisprovide an example of liquid-liquid phase separation chromatin transitions during spermiogenesis

The protamines of the noble false widow spiderSteatoda nobilisprovide an example of liquid-liquid phase separation chromatin transitions during spermiogenesis

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