Dna-Glucosylation in T-Even Phage: Genetic Determination and Role in Phage-Host Interaction

Revel, Helen R.; Luria, Salvador E.

doi:10.1146/annurev.ge.04.120170.001141

Cited by 110 publications

(51 citation statements)

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“…HMC residues, rather than long base sequences unique to the T-even bacteriophage (11). Our results show that the restriction functions can recognize HMC residues in E. coli DNA.…”

Section: Discussionmentioning

confidence: 76%

“…Two independent genes control the restricting functions in E. coli K12 (10,11). The r6 function restricts all T* phages, while the r2,4 function restricts T*2 and T*4 phages, but not T*6 phage.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, DNA synthesized before the addition of HMdCTP is not degraded in the presence of HMdCTP, suggesting that the restriction enzymes are truly specific for HMC. Not all nonglucosylated HMC residues can be recognized, however; phage T6 DNA contains 25% nonglucosylated EIMC residues, and yet is not subject to restriction (11). Georgopoulos and Revel (19) have recently concluded that specific, nonglucosylated, nucleotide sequences are the substrate for restriction, based on studies with phage containing amber and other mutations in the glucosyl-transferase enzymes.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the conditions of repair synthesis do not discriminate between HMdCTP and dCTP. If correct, this model of T* restriction would explain the failure to detect the restriction activities in cell-free extracts when exogenous T* DNA substrates are used (11).…”

Section: Discussionmentioning

confidence: 99%

“…Revel (10) has isolated mutants of E. coli B and K12 that are permissive to T* phages, and by genetic analysis has shown that the restriction of T* phages is controlled by two independent genes: r6 and r2,4. Attempts to demonstrate the restriction functions in cell extracts or in membrane preparations, however, have thus far failed (11). Although some of the deoxyribonucleases found in restrictive strains hydrolyze T*-phage DNA at a much greater rate than glucosylated DNA, these nucleases are present in normal amounts in permissive bacteria (12).…”

mentioning

confidence: 99%

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Analysis of Host Range Restriction in Escherichia coli Treated with Toluene

Fleischman

Richardson

1971

Proc. Natl. Acad. Sci. U.S.A.

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“…HMC residues, rather than long base sequences unique to the T-even bacteriophage (11). Our results show that the restriction functions can recognize HMC residues in E. coli DNA.…”

Section: Discussionmentioning

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Analysis of Host Range Restriction in Escherichia coli Treated with Toluene

Fleischman

Richardson

1971

Proc. Natl. Acad. Sci. U.S.A.

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Binding of histone H1 to DNA is described by an allosteric model

2004

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Equilibrium binding data were analyzed to characterize the interaction of the linker histone H1 degrees with unmodified T4 phage DNA. Data were cast into the Scatchard-type plot described by McGhee and von Hippel and fit to their eponymous model for nonspecific binding of ligand to DNA. The data were not fit by the simple McGhee-von Hippel model, nor fit satisfactorily by the inclusion of a cooperativity parameter. Instead, the interaction appeared to be well described by Crothers' allosteric model, in which the higher affinity of the protein for one conformational form of the DNA drives an allosteric transition of the DNA to the conformational form with higher affinity (form 2). At 214 mM Na(+), the observed affinity K for an isolated site on unmodified T4 bacteriophage DNA in the form 2 conformation is 4.5 x 10(7) M(-1). The binding constant for an isolated site on DNA in the conformation with lower affinity, form 1, appears to be about 10-fold lower. Binding affinity is dependent on ion concentration: the magnitude of K is about 10-fold higher at 14 mM (5.9 x 10(8) M(-1) for form 2 DNA) than at 214 mM Na(+) concentration.

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Genes 55, alpha gt, 47 and 46 of bacteriophage T4: the genomic organization as deduced by sequence analysis.

Gram¹,

Rüger²

1985

The EMBO Journal

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The nucleotide sequence of T4 genes 55, alpha gt, 47 and 46 was determined by a combination of ‘classical’ procedures and a shotgun approach. Small DNA fragments generated by frequent cleavage with restriction enzymes or by sonication of restriction fragments were cloned in phage M13 vectors and sequenced by the dideoxy method. The positions of the genes were determined by marker rescue between the corresponding T4 amber mutants and the cloned T4 DNA fragments used in the sequencing experiments. The sequence gives an insight into the organization of this 7.1‐kb early region of the T4 genome and shows that genetically ‘silent’ portions within this region are not void of genetic information.

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Dna-Glucosylation in T-Even Phage: Genetic Determination and Role in Phage-Host Interaction

Cited by 110 publications

References 0 publications

Analysis of Host Range Restriction in Escherichia coli Treated with Toluene

Analysis of Host Range Restriction in Escherichia coli Treated with Toluene

Binding of histone H1 to DNA is described by an allosteric model

Genes 55, alpha gt, 47 and 46 of bacteriophage T4: the genomic organization as deduced by sequence analysis.

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