Dna-Genotyping of Spring Barley to Loose Smut Resistance by PCR Method

Abstract

The most economical and environmentally safe way to control the disease is to use resistant barley varieties of plants. The use of molecular markers, associated with disease resistance genes, predetermine great possibilities, being used as a tool for the selection of breeders. The study aim is to identify varietal specimen, carrying genes, which are encoding resistance to loose smut of barley. A method of DNA-genotyping by PCR markers was used in the study. During the process of the research 83 varietal specimen have been tested, which were selected in the nursery Tatar Research Institute of Agriculture. The following two SCAR-markers, resistant to loose smut: Un8-700R and Un8-700 were used to identify the U8 gene. The studies found, that 36.2% have the gene, encoding the susceptibility to loose smut, in the genome alleles. 5.3% of the total number of varietal specimen was characterized by the presence of the resistance gene Un8 in the genome allele. The other specimen was observed no resistance gene to loose smut. The comparison with accounting data of loose smut spread, provided by the Barley breeding Department, showed no infestation of barley lines with resistance gene of loose smut in natural infectious background. As a result of molecular and genetic evaluation, the five specimen of nursery of Tatar Research Institute of Agriculture were identified with resistance genes in the genome alleles, which had been recommended, as a valuable genotypes for selection according to the loose smut resistance of barley.