Triple-negative
breast cancer (TNBC) is highly challenging
in its
treatment because of the lack of the targeted markers. TNBC patients
are not able to acquire benefits from endocrine therapy and targeted
therapy except for chemotherapy. CXCR4 is highly expressed on TNBC
cells that mediated the tumor cell metastasis as well as proliferation
by the response of its ligand CXCL12, therefore holding promising
potential of a candidate target for the treatment. In this work, a
novel conjugate of CXCR4 antagonist peptide E5 and gold nanorods was
fabricated (AuNRs-E5), which was applied to murine breast cancer tumor
cells and an animal model, aiming to induce endoplasmic reticulum
stress by endoplasmic reticulum-targeted photothermal immunological
effects. Results showed that AuNRs-E5 could induce much more generation
of damage-related molecular patterns in 4T1 cells under laser irradiation
than AuNRs, which significantly promoted the maturation of dendritic
cells and stimulated systematic anti-tumor immune responses by enhancing
the infiltration of CD8+T cells into the tumor and tumor-draining
lymph node, downregulating the regulatory T lymphocytes, and upregulating
M1 macrophages in tumors, reversing the microenvironment from “cold”
tumors to “hot” tumors. The administration of AuNRs-E5
with laser irradiation not only inhibited the tumor growth significantly
but also exerted specific long immune responses to the triple-negative
breast cancer tumor cells, which led to the prolonged survival of
the mice and the specific immunological memory.