DNA-functionalized dye-loaded carbon dots: ultrabright FRET platform for ratiometric detection of Hg(II) in serum samples and cell microenvironment

Hamd-Ghadareh, Somayeh; Salimi, Abdollah

doi:10.1007/s11581-019-02999-2

Cited by 12 publications

(4 citation statements)

References 52 publications

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“…This strategy has been followed to characterize intracellular pH, ion concentration, or reactive oxygen species (ROS) production using ratiometric sensors based on fluorescent organic compounds, [42,44,68] luminescent particles, [69][70][71][72][73][74] and hybrid structures. [75][76][77][78][79] In addition, targeting ability can be achieved by combining the sensor with a specific cellular label or tagging molecule, whose emission can also be used for ratiometric sensing. [80][81][82] Multiple emissions can be utilized to overcome the intrinsic limitations of certain sensors.…”

Section: Sensing Techniquesmentioning

confidence: 99%

Multichannel Fluorescence Microscopy: Advantages of Going beyond a Single Emission

Rodríguez‐Sevilla

Thompson

Jaque

2022

Advanced NanoBiomed Research

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Fluorescent microscopy has enabled the study of intracellular processes and revealed the most intricate details of the subcellular structure. This has benefitted not only the basic biological science, but also has had an impact in numerous biomedical applications. Basic fluorescent sensing techniques use the change in the absolute emission of a fluorescent sensor. This entails some disadvantages as the signal might be influenced by factors not directly related to the process under study (e.g., fluctuations in the excitation source). To overcome these drawbacks, one can use multiple emissions of a single or various fluorophores. There are numerous examples of multichannel fluorescence microscopy techniques that have given rise to numerous ratiometric methods and multiplexing assays. Herein, how the use of multiple emission channels has impacted fluorescence microscopy in terms of speed, sensitivity, and resolution is reviewed. Using recent examples, how the easy implementation of multichannel detection can overcome current limitations of the main used fluorescence techniques and promote the development of novel microscopy methods is shown.

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Section: Sensing Techniquesmentioning

confidence: 99%

Multichannel Fluorescence Microscopy: Advantages of Going beyond a Single Emission

Rodríguez‐Sevilla

Thompson

Jaque

2022

Advanced NanoBiomed Research

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“…Thus, the Hg(II) was detected by this quenching effect. Salimi and Hamd-Ghadareh [69] designed a fluorescence aptamer sensor system for quantitation of Hg(II) based on the hybridization of T-rich ssDNA immobilized on Rhodamine B(RB)-CDs (RB-CDs-ssDNA) and gold nanoparticles (AuNPs) modified with complementary aptamer (AuNPs-cDNA). By excitation at 450 nm, RB-CDs-ssDNA nanohybrid showed the dual well-resolved emissions at 580 and 668 nm, respectively.…”

Section: Dna Aptamer Affinitymentioning

confidence: 99%

Recent advances in fluorescence probes based on carbon dots for sensing and speciation of heavy metals

2020

Nanophotonics

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Heavy metal (HM) pollution is a major global concern. Carbon dots (CDs) have demonstrated unique properties as sensing platforms for HMs detection. This review summarizes the progress made in recent years in fluorescence methods to determine HMs and their species using CDs. First, the strategies to synthesize and purify CDs are reviewed. The photoluminescence principles of CDs and their sensing mechanisms as HMs sensors are then summarized. The binding strategies between CDs and HMs are proposed to provide salient principles to design desirable CD-based HMs sensors. The preparation and merits of “turn-on” and ratiometric CDs for HMs detection with higher accuracy are discussed compared with commonly used “turn-off” sensors. Subsequently, the progress on detecting single HM ions, multi-HMs, and different metal species in solution, and the development of gel/solid-state sensor platforms such as paper-based devices, sensor arrays, hydrogels, polymer films, and ion-imprinted polymers are critically accessed. Furthermore, the advances in the cell, bacterial, plant, and animal bioimaging of HMs with CDs as promising bioimaging reagents are presented. Finally, the challenges and prospects of CDs as HMs sensors in future investigations are discussed.

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“…Although there is a weak luminescence in pure carbon nanoparticles, a CD with a carbon core and a shell attached to carbonyl, carboxyl, and amino groups shows very strong and adjustable photoluminescence from blue to green lights, however still with poor photoluminescence intensity in the red light . Multiphoton excitation of CD provides up-converted photoluminescence . In addition, the carbon dot has prominent features for biological applications due to facile production, easy surface modification, biological compatibility, low cell toxicity, good stability, good cell permeability, chemical inertness, small size (less than 10 nm), and high solubility in water compared to other quantum dots .…”

Section: Introductionmentioning

confidence: 99%

“…21 Multiphoton excitation of CD provides upconverted photoluminescence. 22 In addition, the carbon dot has prominent features for biological applications due to facile production, easy surface modification, biological compatibility, low cell toxicity, good stability, good cell permeability, chemical inertness, small size (less than 10 nm), and high solubility in water compared to other quantum dots. 23 Therefore, CDs are widely used in a variety of fields such as measurement, biomedical, bioimaging, biosensing, and drug delivery systems and as catalysts, optoelectronic devices, and electroluminescence light emitting diodes.…”

Section: Introductionmentioning

confidence: 99%

Transport Properties of a Molybdenum Disulfide and Carbon Dot Nanohybrid Transistor and Its Applications as a Hg²⁺ Aptasensor

Majd

Ghasemi

Salimi

et al. 2020

ACS Appl. Electron. Mater.

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Unique electronic, optical, and mechanical properties of molybdenum disulfide (MoS2) hold great potential and can be harnessed as a key component in novel applications in electronics, optoelectronics, high-performance sensing material, and photodetection. Carbon quantum dots (CDs) have attracted a great deal of attention because of their biocompatibility, high quantum yield, and tunable light-emission band for biological and photodetector applications. In this research, we used these two materials for the Hg2+ sensor. First, the sensing layer of MoS2 and carbon dots were prepared through sequential solvent exchange and hydrothermal methods, respectively; then, they were characterized. Since mercury, as one of the most harmful pollutants in water, has a significant negative impact on human health and its detection is of great significance, the proposed MoS2 field-effect transistor sensor functionalized with the DNA–CD hybrid was fabricated for the ultrasensitive detection of the Hg2+ ion in an aqueous environment. Specific T-rich DNA was used in the hybrid structure as the capture probe for label-free detection. The DNA-CD/MoS2 hybrid aptasensor was capable of the detection of Hg2+ in a concentration range from 1 aM to 10 pM with a detection limit of 0.65 aM. The effect of the DNA presence in Hg2+ detection was investigated, and the specificity of the aptasensor response to the Hg2+ ion was also evaluated in the presence of other common cations in the aqueous medium. Finally, the aptasensor was used for Hg2+ detection from samples of tap and mineral water with satisfactory results.

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DNA-functionalized dye-loaded carbon dots: ultrabright FRET platform for ratiometric detection of Hg(II) in serum samples and cell microenvironment

Cited by 12 publications

References 52 publications

Multichannel Fluorescence Microscopy: Advantages of Going beyond a Single Emission

Multichannel Fluorescence Microscopy: Advantages of Going beyond a Single Emission

Recent advances in fluorescence probes based on carbon dots for sensing and speciation of heavy metals

Transport Properties of a Molybdenum Disulfide and Carbon Dot Nanohybrid Transistor and Its Applications as a Hg²⁺ Aptasensor

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DNA-functionalized dye-loaded carbon dots: ultrabright FRET platform for ratiometric detection of Hg(II) in serum samples and cell microenvironment

Cited by 12 publications

References 52 publications

Multichannel Fluorescence Microscopy: Advantages of Going beyond a Single Emission

Multichannel Fluorescence Microscopy: Advantages of Going beyond a Single Emission

Recent advances in fluorescence probes based on carbon dots for sensing and speciation of heavy metals

Transport Properties of a Molybdenum Disulfide and Carbon Dot Nanohybrid Transistor and Its Applications as a Hg2+ Aptasensor

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Product

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Transport Properties of a Molybdenum Disulfide and Carbon Dot Nanohybrid Transistor and Its Applications as a Hg²⁺ Aptasensor