DNA-functionalized colloids: Physical properties and applications

Geerts, Nienke; Eiser, Erika

doi:10.1039/c001603a

Cited by 140 publications

(172 citation statements)

References 167 publications

(219 reference statements)

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“…The spread of the population can have an effect on the reaction kinetics, stability and sensitivity of nanoparticle based assays [36][37][38] . To build up a true measure of the spread of zeta potential values for a given particle population, the zeta potential of each individual particle has to be measured, and this aspect is challenging, although electrophoretic and electrochemical techniques allow insight to these measurements 29,39 .…”

Section: Introductionmentioning

confidence: 99%

Particle-by-Particle Charge Analysis of DNA-Modified Nanoparticles Using Tunable Resistive Pulse Sensing

2016

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Resistive pulse sensors, RPS, are allowing the transport mechanism of molecules, proteins and even nanoparticles to be characterised as they traverse pores. Previous work using RPS has shown that the size, concentration and zeta potential of the analyte can be measured. Here we use tunable resistive pulse sensing (TRPS) which utilises a tunable pore to monitor the translocation times of nanoparticles with DNA modified surfaces. We start by demonstrating that the translocation times of particles can be used to infer the zeta potential of known standards and then apply the method to measure the change in zeta potential of DNA modified particles. By measuring the translocation times of DNA modified nanoparticles as a function of packing density, length, structure, and hybridisation time, we observe a clear difference in zeta potential using both mean values, and population distributions as a function of the DNA structure. We demonstrate the ability to resolve the signals for ssDNA, dsDNA, small changes in base length for nucleotides between 15-40 bases long and even the discrimination between partial and fully complementary target sequences. Such a method has potential and applications in sensors for the monitoring of nanoparticles in both medical and environmental samples.

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Section: Introductionmentioning

confidence: 99%

Particle-by-Particle Charge Analysis of DNA-Modified Nanoparticles Using Tunable Resistive Pulse Sensing

2016

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“…DNA origamis [15]. DNA can also be used to functionalise colloids by grafting single-stranded DNA (ss-DNA) molecules on the surface of the particles [16][17][18]. By choosing strands terminating with complementary sequences, the particles can bind to each other via DNA hybridisation and self-assemble into disordered or ordered structures [17,[19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Accurate phase diagram of tetravalent DNA nanostars

Rovigatti

Bomboi

Sciortino

2014

The Journal of Chemical Physics

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We evaluate, by means of molecular dynamics simulations employing a realistic DNA coarsegrained model, the phase behaviour and the structural and dynamic properties of tetravalent DNA nanostars, i.e. nanoconstructs completely made of DNA. We find that, as the system is cooled down, tetramers undergo a gas-liquid phase separation in a region of concentrations which, if the difference in salt concentration is taken into account, is comparable with the recently measured experimental phase diagram [S. Biffi et al, Proc. Natl. Acad. Sci, 110, 15633 (2013)]. We also present a meanfield free energy for modelling the phase diagram based on the bonding contribution derived by Wertheim in his studies of associating liquids. Combined with mass-action law expressions appropriate for DNA binding and a numerically evaluated reference free energy, the resulting free energy qualitatively reproduces the numerical data. Finally, we report information on the nanostar structure, e.g. geometry and flexibility of the single tetramer and of the collective behaviour, providing a useful reference for future small angle scattering experiments, for all investigated temperatures and concentrations.

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“…The sequence specificity, binding fidelity and mechanical rigidity of the DNA molecule have made it a promising building block for the construction of new materials [33][34][35] and devices [36]. DNA has also been conjugated to other particles to direct their assembly into higher-order superstructures [37,38], including encoding the delicate balance between attractive and repulsive interactions required to drive crystallisation in low density colloidal systems [39][40][41]. Hydrophobic modifications to DNA strands have recently been used to for the higher-order assembly of DNA-cages and the fabrication of DNA cages with hydrophobic cores that can host poorly water soluble guest compounds [42].…”

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confidence: 99%