DNA-functionalized carbon quantum dots for electrochemical detection of pyocyanin: A quorum sensing molecule in Pseudomonas aeruginosa

Thulasinathan, Boobalan; Sujatha, D; Murugan, S.; Panda, Subhendu K.; Veerapandian, Murugan; Manickam, Pandiaraj

doi:10.1016/j.bios.2023.115156

Cited by 16 publications

(4 citation statements)

References 48 publications

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“…The exceptional optical properties of carbon quantum dots make them ideal candidates for biosensing applications [56]. Researchers have developed CQD-based biosensors for detecting various analytes, such as glucose, ions, and pathogens [11,[57][58][59]. Functionalization of CQDs with biomolecules or specific receptors enables the selective and sensitive detection of target analytes, contributing to the development of rapid and accurate diagnostic tools [60].…”

Section: Bio-sensing and Diagnosticsmentioning

confidence: 99%

Emerging Trends in Nanomedicine: Carbon-Based Nanomaterials for Healthcare

Parvin,

Kumar,

Joo

et al. 2024

Nanomaterials

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Carbon-based nanomaterials, such as carbon quantum dots (CQDs) and carbon 2D nanosheets (graphene, graphene oxide, and graphdiyne), have shown remarkable potential in various biological applications. CQDs offer tunable photoluminescence and excellent biocompatibility, making them suitable for bioimaging, drug delivery, biosensing, and photodynamic therapy. Additionally, CQDs’ unique properties enable bioimaging-guided therapy and targeted imaging of biomolecules. On the other hand, carbon 2D nanosheets exhibit exceptional physicochemical attributes, with graphene excelling in biosensing and bioimaging, also in drug delivery and antimicrobial applications, and graphdiyne in tissue engineering. Their properties, such as tunable porosity and high surface area, contribute to controlled drug release and enhanced tissue regeneration. However, challenges, including long-term biocompatibility and large-scale synthesis, necessitate further research. Potential future directions encompass theranostics, immunomodulation, neural interfaces, bioelectronic medicine, and expanding bioimaging capabilities. In summary, both CQDs and carbon 2D nanosheets hold promise to revolutionize biomedical sciences, offering innovative solutions and improved therapies in diverse biological contexts. Addressing current challenges will unlock their full potential and can shape the future of medicine and biotechnology.

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Section: Bio-sensing and Diagnosticsmentioning

confidence: 99%

Emerging Trends in Nanomedicine: Carbon-Based Nanomaterials for Healthcare

Parvin,

Kumar,

Joo

et al. 2024

Nanomaterials

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“…Therefore, detecting PYO in respiratory samples, within minutes and at the bedside, could reveal the presence of Pseudomonas and help personalize antibiotic options. Recent works have reported several approaches for detecting PYO, including electrochemical sensors based on paper, gold nanoparticles (AuNPs), or carbon quantum dots [ 10 – 14 ], ELISA [ 15 , 16 ], surface-enhanced Raman spectroscopy (SERS) [ 17 – 19 ], and heat-transfer methods [ 20 ]. Some approaches used antibodies for the specific recognition of PYO, whereas others relied on the electrochemical properties or spectral fingerprint of the molecule to detect it.…”

Section: Introductionmentioning

confidence: 99%

A paper biosensor for overcoming matrix effects interfering with the detection of sputum pyocyanin with competitive immunoassays

Adrover-Jaume,

Clemente,

Rodríguez-Urretavizcaya

et al. 2023

Microchim Acta

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Detecting sputum pyocyanin (PYO) with a competitive immunoassay is a promising approach for diagnosing Pseudomonas aeruginosa respiratory infections. However, it is not possible to perform a negative control to evaluate matrix-effects in competitive immunoassays, and the highly complex sputum matrix often interferes with target detection. Here, we show that these issues are alleviated by performing competitive immunoassays with a paper biosensor. The biosensing platform consists of a paper reservoir, which contains antibody-coated gold nanoparticles, and a substrate containing a competing recognition element, which is a piece of paper modified with an albumin-antigen conjugate. Detection of PYO with a limit of detection of 4.7·10−3 µM and a dynamic range between 4.7·10−1 µM and 47.6 µM is accomplished by adding the sample to the substrate with the competing element and pressing the reservoir against it for 5 min. When tested with patient samples, the biosensor was able to qualitatively differentiate spiked from non-spiked samples, whereas ELISA did not show a clear cut-off between them. Furthermore, the relative standard deviation was lower when determining sputum with the paper-based biosensor. These features, along with a mild liquefaction step that circumvents the use of harsh chemicals or instruments, make our biosensor a good candidate for diagnosing Pseudomonas infections at the bedside through the detection of sputum PYO. Graphical Abstract

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“…Nonetheless, previous studies have explored using additional semiconducting and conductive nanomaterials as composites with carbon nanomaterials to enhance the sensitivity of the immunosensor. In a recent development, Thulasinathan et al presented a two-step synthesis approach to prepare amino-functionalized carbon quantum dots, to develop a precision electrochemical biosensor targeting Pseudomonas aeruginosa …”

mentioning

confidence: 99%

“…In a recent development, Thulasinathan et al presented a two-step synthesis approach to prepare amino-functionalized carbon quantum dots, to develop a precision electrochemical biosensor targeting Pseudomonas aeruginosa. 33 In this study, carbon-based semiconducting nanoparticles bearing amino functional groups were synthesized through a one-step green hydrothermal carbonization process using natural polymer chitosan. The inherent amino and hydroxylic groups present in chitosan's polymeric framework contribute to the preparation of nanoparticles.…”

mentioning

confidence: 99%

Green-Synthesized Amino Carbons for Impedimetric Biosensing of E. coli O157:H7

Sahu,

Gangwar,

Ramesh

et al. 2024

ACS Infect. Dis.

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This study describes the synthesis of amino-functionalized carbon nanoparticles derived from biopolymer chitosan using green synthesis and its application toward ultrasensitive electrochemical immunosensor of highly virulent Escherichia coli O157:H7 (E. coli O157:H7). The inherent advantage of high surface-to-volume ratio and enhanced rate transfer kinetics of nanoparticles is leveraged to push the limit of detection (LOD), without compromising on the selectivity. The prepared carbon nanoparticles were systematically characterized by employing CO 2 -thermal programmed desorption (CO 2 -TPD), Fourier transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), ultraviolet−visible (UV−visible), and transmission electron microscopy (TEM). The estimated limit of detection of 0.74 CFU/mL and a sensitivity of 5.7 ((ΔR ct /R ct )/(CFU/mL))/cm 2 in the electrochemical impedance spectroscopy (EIS) affirm the utility of the sensor. The proposed biosensor displayed remarkable selectivity against interfering species, making it well suited for real-time applications. Moreover, the chitosan-derived semiconducting amino-functionalized carbon shows excellent sensitivity in a comparative analysis compared to highly conducting aminefunctionalized carbon synthesized via chemical modification, demonstrating its vast potential as an E. coli sensor.

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DNA-functionalized carbon quantum dots for electrochemical detection of pyocyanin: A quorum sensing molecule in Pseudomonas aeruginosa

Cited by 16 publications

References 48 publications

Emerging Trends in Nanomedicine: Carbon-Based Nanomaterials for Healthcare

Emerging Trends in Nanomedicine: Carbon-Based Nanomaterials for Healthcare

A paper biosensor for overcoming matrix effects interfering with the detection of sputum pyocyanin with competitive immunoassays

Green-Synthesized Amino Carbons for Impedimetric Biosensing of E. coli O157:H7

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