DNA from Leaf or Stem: A Comparative Work on Dianthus L. for DNA Barcoding Analysis with Four Commercial Extraction Kits

Aydın, Zübeyde Uğurlu; Şenova, Mustafa K.; Koch, Marcus A.; Dönmez, Alı A.

doi:10.15671/hjbc.663505

Cited by 2 publications

References 26 publications

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Effect of plant tissues on DNA quantity and quality of barley (Hordeum vulgare) validating through PCR technique

Kebede

Haile

Palanivel

2021

Preprint

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The use of molecular techniques to deal with plant molecular breeding requires the extraction of genomic DNA in good quantity and quality, which can be influenced by method of extraction and source of DNA (plant species, plant part or tissues). However, this research focuses on plant tissue source and tried to describe the quality and quantity of DNA isolated from different tissues’ of barley crop. CTAB protocol was used for the isolation of DNA and both the quality and quantity of this DNA was validated through gel electrophoresis, Qubit quantification and PCR amplifications. The result showed that DNA could be successfully extracted from all tissues of plants and the yield of DNA obtained was variable ranging from 179ng μl-1 in stem to 750ng μl-1 in young leave. Band intensity of genomic and PCR amplified DNA was good for DNA isolated from young and matured leave. Faint band was observed in the PCR amplification for DNA isolated from stem but no to unreliable amplification was obtained for seeds and roots, respectively. Thus, for any molecular technique in barley crop research, the best tissue for DNA isolation using modified CTAB, is young or matured leave and alternatively stem can be used as DNA source.

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Effect of plant tissues on DNA quantity and quality of barley (Hordeum vulgare) validating through PCR technique

Kebede

Haile

Palanivel

2021

Preprint

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Phytochemical composition, biological activities and nutritional aspects of <i>Hylocereus undatus</i>: a review

Mande,

Kumbhare,

Surana

2023

Infect Dis Herbal Med

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The most commonly cultivated vine cactus in the Cactaceae family is normally Hylocereus undatus, which would be native to Mexico and America. It is commonly referred to as "dragon fruit" or "pitaya." Additionally, it goes by the name "buahnaga," which means "dragon fruit." In addition to their attractive color, Hylocereus undatus fruits are popular around the world due to their abundance of polyphenolic components and their antioxidant activity. Due to the characteristics, degree of production, and financial benefits of the pitahaya fruit, a member of the Hylocereus genus, more land has been planted in agriculture during the past two decades. Stalks, fruits, and flowers from the plant are used in food and film.

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DNA from Leaf or Stem: A Comparative Work on Dianthus L. for DNA Barcoding Analysis with Four Commercial Extraction Kits

Cited by 2 publications

References 26 publications

Effect of plant tissues on DNA quantity and quality of barley (Hordeum vulgare) validating through PCR technique

Effect of plant tissues on DNA quantity and quality of barley (Hordeum vulgare) validating through PCR technique

Phytochemical composition, biological activities and nutritional aspects of <i>Hylocereus undatus</i>: a review

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