DNA-free genome editing in tomato protoplasts using CRISPR/Cas9 ribonucleoprotein delivery

Kang, Gagandeep; Kang, Beum‐Chang; Han, Jeung-Sul; Lee, Je Min

doi:10.1007/s13580-023-00549-4

Cited by 2 publications

(2 citation statements)

References 67 publications

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“…This approach relies on the establishment of successful protoplast isolation and regeneration procedures. PEG-mediated transfection has been widely used to deliver vector DNA or RNPs into protoplasts of many plant species, including various tomato cultivars [ 78 , 79 , 80 , 81 ] and the wild tomato S. peruvianum [ 82 ].…”

Section: Methods For Obtaining Gene-edited Plantsmentioning

confidence: 99%

Recent Advances in Tomato Gene Editing

Larriba,

Yaroshko,

Pérez-Pérez

2024

IJMS

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The use of gene-editing tools, such as zinc finger nucleases, TALEN, and CRISPR/Cas, allows for the modification of physiological, morphological, and other characteristics in a wide range of crops to mitigate the negative effects of stress caused by anthropogenic climate change or biotic stresses. Importantly, these tools have the potential to improve crop resilience and increase yields in response to challenging environmental conditions. This review provides an overview of gene-editing techniques used in plants, focusing on the cultivated tomatoes. Several dozen genes that have been successfully edited with the CRISPR/Cas system were selected for inclusion to illustrate the possibilities of this technology in improving fruit yield and quality, tolerance to pathogens, or responses to drought and soil salinity, among other factors. Examples are also given of how the domestication of wild species can be accelerated using CRISPR/Cas to generate new crops that are better adapted to the new climatic situation or suited to use in indoor agriculture.

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Section: Methods For Obtaining Gene-edited Plantsmentioning

confidence: 99%

Recent Advances in Tomato Gene Editing

Larriba,

Yaroshko,

Pérez-Pérez

2024

IJMS

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“…Transformation can be achieved through various methods, including electroporation, PEG-induced transformation, and Agrobacterium -mediated delivery. These include model plants such as Arabidopsis thaliana [ 74 , 79 , 80 , 81 ], and a range of food crops such as tomatoes [ 82 , 83 ], cabbage [ 84 , 85 ], broccoli [ 86 ], carrots [ 87 , 88 ], maize [ 89 , 90 , 91 ], soybeans [ 92 , 93 , 94 ], and rice [ 95 ], as well as cash crops such as sugarcane [ 96 ], bananas [ 97 ], strawberries [ 98 , 99 ], and apples [ 100 ]. Although genetic transformation technologies, somatic cell fusion, and protoplast transient expression have advanced in research related to Brassica napus , there is a relative lack of comprehensive reviews focusing on the specifics of genetic transformation and gene editing in this important crop.…”

Section: Introductionmentioning

confidence: 99%

Advances in and Perspectives on Transgenic Technology and CRISPR-Cas9 Gene Editing in Broccoli

Zhang,

Meng,

Liu

et al. 2024

Genes

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Broccoli, a popular international Brassica oleracea crop, is an important export vegetable in China. Broccoli is not only rich in protein, vitamins, and minerals but also has anticancer and antiviral activities. Recently, an Agrobacterium-mediated transformation system has been established and optimized in broccoli, and transgenic transformation and CRISPR-Cas9 gene editing techniques have been applied to improve broccoli quality, postharvest shelf life, glucoraphanin accumulation, and disease and stress resistance, among other factors. The construction and application of genetic transformation technology systems have led to rapid development in broccoli worldwide, which is also good for functional gene identification of some potential traits in broccoli. This review comprehensively summarizes the progress in transgenic technology and CRISPR-Cas9 gene editing for broccoli over the past four decades. Moreover, it explores the potential for future integration of digital and smart technologies into genetic transformation processes, thus demonstrating the promise of even more sophisticated and targeted crop improvements. As the field continues to evolve, these innovations are expected to play a pivotal role in the sustainable production of broccoli and the enhancement of its nutritional and health benefits.

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DNA-free genome editing in tomato protoplasts using CRISPR/Cas9 ribonucleoprotein delivery

Cited by 2 publications

References 67 publications

Recent Advances in Tomato Gene Editing

Recent Advances in Tomato Gene Editing

Advances in and Perspectives on Transgenic Technology and CRISPR-Cas9 Gene Editing in Broccoli

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