DNA fragmentation index (DFI) as a measure of sperm quality and fertility in mice

Li, Ming Wen; Lloyd, Kevin C K

doi:10.1038/s41598-020-60876-9

Cited by 27 publications

(26 citation statements)

References 67 publications

(95 reference statements)

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“…Among all the studies included in this systematic review, only two [53,60] using mice analyzed implantation rates after ICSI procedures, finding a reduction of this parameter. Li et al [53] and Li and Lloyd [54] also reported similar findings regarding live birth rates; these studies were the only ones which analyzed this parameter.…”

Section: Effect Of the Different Treatments On Ivf/icsi Outcomesmentioning

confidence: 54%

“…With regard to studies performing treatments, three applied heat stress, three applied radiation (X rays or Gamma rays), seven treated sperm with oxidant molecules, such as H 2 O 2 or xanthine oxidase, one applied genetic modifications creating mouse chimeras for Protamine 2 (Prm2) gene, one caused damage through freeze-thawing, and eleven performed treatments with other chemical agents, such as manganese and calcium, lipopolysaccharide, cyclophosphamide, alkaline pH, fluoride, pyridaben, and DAVA, or fed animals with a selenium-deficient diet. 17 Li et al 2020 [54] To assess the extent to which the measurement of DNA fragmentation index in sperm can predict their fertilizing ability.…”

Section: Effect Of Different Treatments On Spermatozoamentioning

confidence: 99%

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The Relationship between Sperm Oxidative Stress Alterations and IVF/ICSI Outcomes: A Systematic Review from Nonhuman Mammals

Ribas-Maynou

Yeste

Salas‐Huetos

2020

Biology

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Achieving high embryo quality following IVF and ICSI procedures is a key factor in increasing fertility outcomes in human infertile couples. While the male factor is known to underlie infertility in about 50% of cases, studies performed in human infertile couples have not been able to define the precise effect of sperm affectations upon embryo development. This lack of consistency is, in most cases, due to the heterogeneity of the results caused by the multiple male and female factors that mask the concrete effect of a given sperm parameter. These biases can be reduced with the use of animal gametes, being a good approach for basic researchers to design more homogeneous studies analyzing the specific consequences of a certain affectation. Herein, we conducted a systematic review (March 2020) that assessed the relationship between sperm oxidative stress alterations and IVF/ICSI outcomes in nonhumans mammals. The review was conducted according to PRISMA guidelines and using the MEDLINE-PubMed and EMBASE databases. Thirty articles were included: 11 performed IVF, 17 conducted ICSI, and two carried out both fertilization methods. Most articles were conducted in mouse (43%), cattle (30%) and pig models (10%). After IVF treatments, 80% of studies observed a negative effect of sperm oxidative stress on fertilization rates, and 100% of studies observed a negative effect on blastocyst rates. After ICSI treatments, a positive relationship of sperm oxidative stress with fertilization rates (75% of studies) and with blastocyst rates (83% of studies) was found. In conclusion, the present systematic review shows that sperm oxidative stress is associated with a significant reduction in fertilization rates and in vitro embryo development.

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Section: Effect Of the Different Treatments On Ivf/icsi Outcomesmentioning

confidence: 54%

Section: Effect Of Different Treatments On Spermatozoamentioning

confidence: 99%

The Relationship between Sperm Oxidative Stress Alterations and IVF/ICSI Outcomes: A Systematic Review from Nonhuman Mammals

Ribas-Maynou

Yeste

Salas‐Huetos

2020

Biology

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“…Normally, reductions in fertilization rate, embryo quality, implantation rate, chromosomal aberration, and repeated abortions are associated with sperm DNA fragmentation. Several studies indicated the usefulness of sperm DNA integrity as a tool for fertility assessment (13,81,178,182). In contrast, %DFI was reported to have a low impact on fertilization (184), implantation rates (185), and embryo quality (186).…”

Section: Prognostic Value Of Sperm Dna Qualitymentioning

confidence: 99%

Sperm DNA Integrity and Male Fertility in Farm Animals: A Review

et al. 2020

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The accurate prediction of male fertility is of major economic importance in the animal breeding industry. However, the results of conventional semen analysis do not always correlate with field fertility outcomes. There is evidence to indicate that mammalian fertilization and subsequent embryo development depend, in part, on the inherent integrity of the sperm DNA. Understanding the complex packaging of mammalian sperm chromatin and assessment of DNA integrity could potentially provide a benchmark in clinical infertility. In the era of assisted reproduction, especially when in-vitro fertilization or gamete intrafallopian transfer or intracytoplasmic sperm injection is used, assessment of sperm DNA integrity is important because spermatozoa are not subjected to the selection process occurring naturally in the female reproductive tract. Although sperm DNA integrity testing measures a significant biological parameter, its precise role in the infertility evaluation in farm animals remains unclear. In this review, the earlier findings on sperm DNA integrity in relation to male fertility are compiled and analyzed. Furthermore, the causes and consequences of sperm DNA damage are described, together with a review of advances in methods for detection of sperm DNA damage, and the prognostic value of sperm DNA quality on male fertility.

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“…Another theory for the pathophysiology of infertility in varicocele patients is related to increased oxidative stress. This has been linked to sperm DNA damage, including DNA fragmentation, and correlated with the decreased capacity of spermatozoa to fertilise oocytes during normal fertilisation and assisted reproduction techniques (ARTs, in vitro fertilisation and intracytoplasmic sperm injection [ICSI]) [ 6 , 9 ]. Studies have also reported a higher prevalence of DNA fragmentation in varicocele patients and a correlation between the presence of varicocele and impaired sperm DNA integrity [ 7 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

The effects of varicocelectomy on the DNA fragmentation index and other sperm parameters: a meta-analysis

Birowo

Wijaya

Atmoko

et al. 2020

Basic Clin. Androl.

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Background Varicocele is one of the most common causes of reversible male infertility, and 15% of the varicocele patients with normal semen analysis are diagnosed as infertile. According to the current guidelines, varicocelectomy is indicated based on abnormal sperm parameters and not abnormal DNA fragmentation index (DFI) values. Thus, in this study, we performed a meta-analysis of the effects of varicocelectomy on the DFI and other conventional sperm parameters, and determined whether DFI could be used to indicate varicocelectomy for varicocele patients. Results Through an electronic search of the PubMed, Scopus, EBSCO, and Cochrane databases, we included 7 prospective studies including a total of 289 patients in this meta-analysis. The results showed that varicocelectomy significantly reduced DNA fragmentation (mean difference: − 6.86; 95% confidence interval [CI]: − 10.04, − 3.69; p < 0.00001) and improved sperm concentration (mean difference: 9.59; 95% CI: 7.80, 11.38; p < 0.00001), progressive motility (mean difference: 8.66; 95% CI: 6.96, 10.36; p < 0.00001), and morphology (mean difference: 2.73; 95% CI: 0,65, 4.80; p = 0.01). Conclusion Varicocelectomy reduced DNA fragmentation and improved sperm concentration, progressive motility, and morphology. Additionally, the analysis showed that an abnormal DFI measurement should be considered as an indication for varicocelectomy.

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DNA fragmentation index (DFI) as a measure of sperm quality and fertility in mice

Cited by 27 publications

References 67 publications

The Relationship between Sperm Oxidative Stress Alterations and IVF/ICSI Outcomes: A Systematic Review from Nonhuman Mammals

The Relationship between Sperm Oxidative Stress Alterations and IVF/ICSI Outcomes: A Systematic Review from Nonhuman Mammals

Sperm DNA Integrity and Male Fertility in Farm Animals: A Review

The effects of varicocelectomy on the DNA fragmentation index and other sperm parameters: a meta-analysis

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