DNA fragmentation in V79 cells irradiated with light ions as measured by pulsed-field gel electrophoresis. I. Experimental results

Abstract

Differences were observed in the yield and spatial correlation, at a molecular size scale characteristic of loop dimensions, of the double-strand breaks induced by gamma-rays and by light ions. These effects may have a role in the observed different cell response to these radiations.

DNA fragmentation in V79 cells irradiated with light ions as measured by pulsed‐field gel electrophoresis. II. Simulation with a generalized broken stick model

“…For all the details concerning the cell culture, irradiation procedures and DSB measurements, see Belli et al (2002). Only the information useful in the analysis that will be described in the present paper is reported.…”

“…The GBS model has been applied to the experimental data reported in Belli et al (2002) concerning the fragment distributions observed by pulsed-field gel electrophoresis (PFGE) in mammalian V79 cells exposed to c-rays, low-energy protons or 4 He ions.…”

Evaluation of lesion clustering in irradiated plasmid DNA

“…Most experimental studies reported either a relatively weak or no dependence of the DSB yield on LET, depending on the method used (Prise et al 1998(Prise et al , 2001Belli et al 2002). Thus the reported LET dependence for DSB induction has been much lower than that for cell killing.…”

DNA fragmentation induced by Fe ions in human cells: shielding influence on spatially correlated damage

“…In order to evaluate the influence of small fragments when considering a wider range of molecular weights, a cumulative function was used to determine the number of fragments in any interval included between 5700 kbp and any molecular weight considered in the size range 1-5700 kbp (Belli et al, 2002).…”

“…7.5 ml scintillation cocktail (Ultima Gold XR) was added to each sample and the activity was measured using a beta counter. Fragment number and distribution were evaluated as described by Belli et al (2001Belli et al ( , 2002.…”