DNA Flow Cytometry in Non‐Vascular Plants

Voglmayr, Hermann

doi:10.1002/9783527610921.ch12

Cited by 8 publications

(5 citation statements)

References 55 publications

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“…Once we had undoubtedly determined that internal patterns were more suited for 2C value estimation, we moved forward to determine if soybean was indeed the most indicated species to be used as reference pattern as indicated by Praça-Fontes et al 16 For FCM, it has been indicated that standards must have a genome size about 0.4 to 2.5 times the size of the unknown specimen, 23 including in this range both soybean, 'Polanka' with 2C = 2.50 pg and AT = 63.6% 12,13,24 and tomato, S. lycopersicum L. 'Stupické polní rané', 2C = 1.96 pg. 12,13 However, we actually did not find many significant differences in precision (measured by the standard deviations) when comparing soybean and tomato (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid

et al. 2014

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Aiming at generating a comprehensive genomic database on Elaeis spp., our group is leading several R&D initiatives with Elaeis guineensis (African oil palm) and Elaeis oleifera (American oil palm), including the whole-genome sequencing of the last. Genome size estimates currently available for this genus are controversial, as they indicate that American oil palm genome is about half the size of the African oil palm genome and that the genome of the interspecific hybrid is bigger than both the parental species genomes. We estimated the genome size of three E. guineensis genotypes, five E. oleifera genotypes, and two interspecific hybrids genotypes. On average, the genome size of E. guineensis is 4.32 ± 0.173 pg, while that of E. oleifera is 4.43 ± 0.018 pg. This indicates that both genomes are similar in size, even though E. oleifera is in fact bigger. As expected, the hybrid genome size is around the average of the two genomes, 4.40 ± 0.016 pg. Additionally, we demonstrate that both species present around 38% of GC content. As our results contradict the currently available data on Elaeis spp. genome sizes, we propose that the actual genome size of the Elaeis species is around 4 pg and that American oil palm possesses a larger genome than African oil palm.

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Section: Discussionmentioning

confidence: 99%

Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid

et al. 2014

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“…those belonging to Heterokonta, such as diatoms, and the brown algae (Phaeophyta)). They are often neglected because of the methodological challenges they pose when estimating genome size (Voglmayr, ; Mazalová et al , ). Evolutionary relationships among many algal lineages are still controversial, but recent phylogenetic studies provide strong support for three of the streptophyte algal lineages (i.e.…”

Section: Growth Gaps and Novel Insights In Plant Genome Size Diversimentioning

confidence: 99%

The Plant DNA C‐values database (release 7.1): an updated online repository of plant genome size data for comparative studies

2019

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“…Endopolyploidy has also been observed in moss rhizoids and food conducting tissues [28]. The use of flow cytometry to observe endopolyploidy in bryophytes has been referred to anecdotally [29]. Additionally, endopolyploidy has been explored in the model moss, Physcomitrella patens, which has a unique case of exhibiting two distinct ploidy levels in different tissues.…”

Section: Introductionmentioning

confidence: 99%

Endopolyploidy in Bryophytes: Widespread in Mosses and Absent in Liverworts

Bainard

Newmaster

2010

Journal of Botany

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Endopolyploidy occurs when DNA replication is not followed by mitotic nuclear division, resulting in tissues or organisms with nuclei of varying ploidy levels. Endopolyploidy appears to be a common phenomenon in plants, though the prevalence of endopolyploidy has not been determined in bryophytes (including mosses and liverworts). Forty moss species and six liverwort species were analyzed for the degree of endopolyploidy using flow cytometry. Nuclei were extracted in LB01 buffer and stained with propidium iodide. Of the forty moss species, all exhibited endopolyploid nuclei (mean cycle value=0.65±0.038) except for theSphagnummosses (mean cycle value=0). None of the liverwort species had endopolyploid nuclei (mean cycle value= 0.04 ± 0.014). As bryophytes form a paraphyletic grade leading to the tracheophytes, understanding the prevalence and role of endopolyploidy in this group is important.

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DNA Flow Cytometry in Non‐Vascular Plants

Cited by 8 publications

References 55 publications

Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid

Reassessment of the Genome Size in Elaeis guineensis and Elaeis oleifera, and Its Interspecific Hybrid

The Plant DNA C‐values database (release 7.1): an updated online repository of plant genome size data for comparative studies

Endopolyploidy in Bryophytes: Widespread in Mosses and Absent in Liverworts

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