DNA Flow Cytometry Does Not Predict 5- or 10-Year Recurrence Rates for T1-2 Node-Negative Breast Cancer

Abstract

Background: A small proportion of T1 or T2 nodenegative breast cancer tumors will recur in patients by 5 years, and more by 10 years. Results of recent studies have suggested improvement in overall survival with administration of adjuvant chemotherapy to all patients. More sensitive and specific methods are needed to identify patients at highest risk for recurrence who might benefit most from adjuvant therapy, saving others from unnecessary treatment. Some investigators have suggested DNA flow cytometry as a method to discriminate patients at greatest risk for recurrence. Hypothesis: DNA flow cytometry has predictive value for breast cancer recurrence in node-negative patients. Methods: The cancer registry of a medium-sized university-affiliated hospital was used to identify patients with T1-2 N0 M0 breast cancer treated with a uniform surgical approach and no adjuvant therapy who had completed at least 5 years of follow-up or had recurrence. Flow cytometric analysis was performed on paraffin-embedded specimens. Results: Of 115 patients, 92 (80%) had disease-free survival without recurrence and 23 (20%) had recurrence. Comparison of diploid and nondiploid tumors for likelihood of recurrence revealed no association (P=.79). Furthermore, the DNA index and S-phase fraction were not significantly different between recurrent and nonrecurrent groups.

Cuantificación de ADN y CK18 por citometría de flujo multiparamétrica en el cáncer de mama inicial en el tumor primario y ganglios centinelas. Valor pronóstico a largo plazo

“…Otros autores, por el contrario, encuentran que la ploidía y no la FFS demuestra ser factor pronóstico independiente para la SG (240,335,351) o para la SG y la SLE (392,393). Por el contrario, hay autores que afirman que no existe relación pronóstica entre la ploidía y la FFS tanto con la SG y la SLE (394)(395)(396)(397).…”