DNA flow cytometry, clinical and morphological parameters as prognostic factors for advanced malignant and borderline ovarian tumors

Kuhn, Walther; Kaufmann, M; Ge, Feichter; Hh, Rummel; Schmid, H.; Heberling, Dirk

doi:10.1016/0020-7292(90)90221-6

Cited by 3 publications

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“…Several investigators have studied DNA content in ovarian borderline tumors with computerized image analysis or flow cytometry (3)(4)(5)(6)(7)(8)(9)(10)(11)(12). A review of the literature reveals DNA analysis of 303 ovarian borderline tumors Several studies suggest that aneuploidy in borderline tumors is associated with an adverse prognosis ( 5,11,12).…”

Section: Discussionmentioning

confidence: 99%

“…IL) for an additional 60 min, and rinsed with CAS Rinse Reagent in increasing intervals of 30 sec, 5 min, and 10 rnin. The slides were subsequently washed in deionized water for 5 min, placed in 1 % acid alcohol for 5 …”

Section: Image Analysismentioning

confidence: 99%

“…In addition, there is still controversy regarding histologic criteria for differentiating borderline mucinous tumors from low-grade mucinous carcinomas ( I ,2 ). Several studies have demonstrated that evaluation of DNA content in borderline tumors is a useful adjunct to histopathologic diagnosis (5,11,12 ). Although the sample size in these reports has been small, they indicated that the finding of aneuploidy in thcse lesions is associated with an adverse prognosis.…”

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confidence: 99%

“…Although the sample size in these reports has been small, they indicated that the finding of aneuploidy in thcse lesions is associated with an adverse prognosis. Some of those studies included DNA analysis using flow cytornetry of either fresh or paraffin-embedded tissue (5)(6)(7)(8)(9)(10)(11)(12). Other studies have utilized computerized image analysis of Feulgen-stained cell suspensions or paraffin sections to evaluate DNA content C) 1994 Wiley-Liss, Inc (3,4).…”

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Computerized image analysis and flow cytometric evaluation of ovarian bordeline tumors: A study of 24 cases

Esposito¹,

Fuchs²

1994

Cytometry

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DNA content evaluation in the study of ovarian borderline tumors has been shown to be a useful adjunct to histopathologic diagnosis. This study compares flow cytometry and computerized image analysis (CIA) in evaluating the DNA content of these lesions.Twenty-four cases of ovarian borderline tumors (1 5 serous, 9 mucinous) were studied utilizing formalinfixed paraffin-embedded tissue. Flow cytometry of cell suspensions and CIA of cell suspensions and paraffin sections were compared in the evaluation of DNA content.Twenty-three tumors (96%) were diploid and only 1 (4%) was aneuploid. There was 100% correlation between flow cytometry and CIA of cell suspensions. Image analysis of Feulgen-stained paraffin sections was found to be unreliable when compared with the use of cell suspensions in the evaluation of DNA content. Clinical follow-up in 17 patients showed recurrent disease in two patients with diploid tumors and no evidence of disease in the patient with an aneuploid tumor.The reported rate of aneuploidy in borderline tumors varies depending on the diagnostic method employed in evaluating DNA content. This study shows good correlation between flow cytometry and image analysis of cell suspensions and supports the low rate of aneuploidy found in these lesions by other authors utilizing these methods. The majority of borderline ovarian tumors have been shown to have a diploid DNA content; however, the finding of aneuploidy in histologically classified borderline tumors warrants close clinical follow-up since aneuploidy in these tumors has been associated with an adverse prognosis. 0 1994 Wiley-Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

Section: Image Analysismentioning

confidence: 99%

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confidence: 99%

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Computerized image analysis and flow cytometric evaluation of ovarian bordeline tumors: A study of 24 cases

Esposito¹,

Fuchs²

1994

Cytometry

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“…Like other prognosticators, they should be kept in mind for estimating survival and the need for salvage therapies [7]. Proliferative activity as measured in the S-phase fraction has been shown to correlate with prognosis in some studies [8]. DNA flow cytometry is an objective way to determine cellular DNA content and is free of intra-and interobserver variabilities when compared with direct microscopy.…”

Section: Discussionmentioning

confidence: 99%

Effect of Cellular DNA Content on the Prognosis of Epithelial Ovarian Cancers

Özalp

Yalçın

Gülbaş

et al. 2001

Gynecol Obstet Invest

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Objective: To assess the cellular DNA status of epithelial ovarian cancers with regard to clinicopathological findings and its effect on prognosis. Materials and Methods: Twenty-six consecutive patients with a diagnosis of epithelial ovarian cancer who had been treated by primary surgery and six courses of platinum-based chemotherapy were enrolled in this study. Second-look laparotomy (SLL) was performed in all cases following confirmation of the clinical remission state. Surgical stage, tumor grade, initial tumor volume, residual tumor volume, histopathologic differentiation, and SLL findings were analyzed in correlation with DNA ploidy and DNA index. DNA analysis was performed via DNA flow cytometry through paraffin-embedded tissue specimens. Results: Of 26 patients, flow cytometric studies revealed 16 aneuploidy cases (61.5%). DNA index values ranged from 1.1 to 1.82 (average 1.29 ± 0.28). The flow cytometry coefficient of variation mean value was set to 6.7. Taking the cutoff value of 1.2 for DNA indices, a fairly good correlation was detected between DNA ploidy and DNA indices (p < 0.001). The aneuploidy incidence was found to be high in advanced and poorly differentiated tumors (p < 0.05). There was statistically more residual tumor volume in aneuploid tumors during primary cytoreductive surgery and also higher recurrence rates following six courses of chemotherapy compared with diploid tumors (p < 0.05). No significant correlation was detected between the histopathologic subtypes and tumor volume (p > 0.05). Residual tumor volumes were larger in cases with DNA indices of 1.2 yielding higher residual tumor volume following surgery and being in good correlation with SLL results (p < 0.05). The mean survival rates of cases with aneuploid tumor and a DNA index of >1.2 were low compared to those with diploid tumors and DNA indices of <1.2 tumors (p < 0.05). Conclusion: DNA ploidy and DNA indices are important prognosticators for malignant epithelial ovarian tumors. They should be evaluated together with the patient’s clinical status and other prognostic factors.

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Μελέτη Των Επιπέδων Της Καλποπρωτεκτίνης Και Της Προαλβουμίνης Στον Ορό Του Αίματος Ασθενών Με Επιβεβαιωμένο Καρκίνο Των Ωοθηκών

Petsa¹,

Πέτσα²

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Εισαγωγή: Ο καρκίνος των ωοθηκών είναι η τρίτη συχνότερη αιτία θανάτου, που σχετίζεται με κακοήθεια, μεταξύ των γυναικών.Σκοπός: Οι συγκεντρώσεις της καλπροτεκτίνης και της προαλβουμίνης έχουν ήδη συνδεθεί με τον καρκίνο των ωοθηκών. Ωστόσο, μέχρι σήμερα, η αποτελεσματικότητά τους στη διάγνωση της νόσου παραμένει αβέβαιη. Ο σκοπός της παρούσας μελέτης είναι να προσδιοριστεί εάν τα επίπεδα της προαλβουμίνης και της καλπροτεκτίνης συσχετίζονται με το στάδιο και τον ιστολογικό τύπο της διάγνωσης.Υλικά – Μέθοδοι: Στην παρούσα μελέτη, συμπεριλήφθησαν 80 γυναίκες ηλικίας 30 έως 45 ετών, που είτε είχαν πρωτοπαθή καρκίνο των ωοθηκών (στάδια 1 και 2) με υψηλά επίπεδα του Ca – 125 (n = 40), είτε ήταν υγιείς (n = 40 – ομάδα ελέγχου). Τα επίπεδα της καλποπρωτεκτίνης και της προαλβουμίνης αξιολογήθηκαν με ELISA. Η στατιστική ανάλυση πραγματοποιήθηκε με SPSS v.20.0 statistical software.Αποτελέσματα: Τόσο τα επίπεδα της προαλβουμίνης, όσο και τα επίπεδα της καλπροτεκτίνης ήταν σημαντικά διαφορετικά στις ασθενείς με καρκίνο των ωοθηκών, συγκριτικά με την ομάδα ελέγχου. Πιο συγκεκριμένα, τα επίπεδα της προαλβουμίνης στον ορό φάνηκε να ακολουθεί ένα πτωτικό μοτίβο όσο το στάδιο της νόσου γινόταν περισσότερο προχωρημένο [21,2 (15,5 έως 30,6) στην ομάδα ελέγχου, 12,8 (6,9 έως 16) στις ασθενείς με νόσο σταδίου 1 και 9,9 (4,0 έως 15,5) στις ασθενείς με νόσο σταδίου 2, p <0,001]. Η ευαισθησία της ήταν 100% και η ειδικότητά της 82,5%. Αντίθετα, οι τιμές της καλπροτεκτίνης φάνηκε να παρουσιάζουν ένα ανοδικό μοτίβο [550 (100-2780) στην ομάδα ελέγχου, 3320 (2580-7930) στις ασθενείς με νόσο σταδίου 1 και 3825 (2350 έως 5990) στις ασθενείς με νόσο σταδίου 2, p <0,001]. Παρομοίως, η ευαισθησία της ήταν 100% και η ειδικότητά της 77,5%.Συμπεράσματα: Τόσο η προαλβουμίνη, όσο και η καλπροτεκτίνη φαίνεται να αποτελούν χρήσιμους δείκτες για τον προσδιορισμό των ασθενών που πάσχουν από καρκίνο των ωοθηκών.

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DNA flow cytometry, clinical and morphological parameters as prognostic factors for advanced malignant and borderline ovarian tumors

Cited by 3 publications

References 0 publications

Computerized image analysis and flow cytometric evaluation of ovarian bordeline tumors: A study of 24 cases

Computerized image analysis and flow cytometric evaluation of ovarian bordeline tumors: A study of 24 cases

Effect of Cellular DNA Content on the Prognosis of Epithelial Ovarian Cancers

Μελέτη Των Επιπέδων Της Καλποπρωτεκτίνης Και Της Προαλβουμίνης Στον Ορό Του Αίματος Ασθενών Με Επιβεβαιωμένο Καρκίνο Των Ωοθηκών

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