DNA fingerprints of chickens selected for high and low body weight for 31 generations

Dunnington, E. A.; Gal, Orit; Plotsky, Y.; Haberfeld, A.; Kirk, T.A.; Goldberg, Amir; Lavi, Uri; Cahaner, A.; Siegel, P. B.; Hillel, J.

doi:10.1111/j.1365-2052.1990.tb03234.x

Cited by 82 publications

(51 citation statements)

References 19 publications

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“…For instance, the feral populations and the domesticated unselected populations were the most polymorphic, while the inbred and the White Leghorn lines were far less polymorphic. This suggests that use of DNA pools, the chosen markers and the biometrical tools described in this report, provide reliable estimates for the population's biodiversity (see also [10,12,13,24,25,31]). Furthermore, polymorphism estimates obtained from these DNA pools were found to be in good agreement with those obtained from individually typing a subset of 30 birds from 20 populations (unpublished data).…”

Section: Data Reliabilitymentioning

confidence: 79%

“…These markers provide a powerful tool for QTL research, and have also been successfully used to study the genetic relationship between and within chicken populations [36,40,41,45,48]. Reliable information on allele frequencies was obtained from chicken blood or DNA pools using minisatellite markers [12,13,24,25], as well as microsatellites [10,31].…”

Section: Introductionmentioning

confidence: 99%

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Biodiversity of 52 chicken populations assessed by microsatellite typing of DNA pools

Groenen²,

et al. 2003

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-In a project on the biodiversity of chickens funded by the European Commission (EC), eight laboratories collaborated to assess the genetic variation within and between 52 populations from a wide range of chicken types. Twenty-two di-nucleotide microsatellite markers were used to genotype DNA pools of 50 birds from each population. The polymorphism measures for the average, the least polymorphic population (inbred C line) and the most polymorphic population (Gallus gallus spadiceus) were, respectively, as follows: number of alleles per locus, per population: 3.5, 1.3 and 5.2; average gene diversity across markers: 0.47, 0.05 and 0.64; and proportion of polymorphic markers: 0.91, 0.25 and 1.0. These were in good agreement with the breeding history of the populations. For instance, unselected populations were found to be more polymorphic than selected breeds such as layers. Thus DNA pools are effective in the preliminary assessment of genetic variation of populations and markers. Mean genetic distance indicates the extent to which a given population shares its genetic diversity with that of the whole tested gene pool and is a useful criterion for conservation of diversity. The distribution of populationspecific (private) alleles and the amount of genetic variation shared among populations supports the hypothesis that the red jungle fowl is the main progenitor of the domesticated chicken.genetic distance / polymorphism / red jungle fowl / DNA markers / domesticated chicken

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Section: Data Reliabilitymentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Biodiversity of 52 chicken populations assessed by microsatellite typing of DNA pools

Groenen²,

et al. 2003

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“…Band frequency of RAPD fingerprints was determined as the ratio of number of animals (n) carrying a particular band to the total number of animals (N) screened per breed. Band sharing frequency within breed was calculated (Dunnington et al, 1990). Mathematically it could be expressed as BSF = 2Cab / (Na + Nb), where, Cab was the number of common bands showed by individual a and b; Na and Nb were the total number of bands for individual a and b, respectively.…”

Section: Discussionmentioning

confidence: 99%

Genetic Relationship Among Four Indian Breeds of Sheep Using RAPD-PCR

Kumar

Bhattacharya

et al. 2003

Journal of Applied Animal Research

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“…Genomic DNA was prepared from erythrocytes by using a standard phenol/chloroform isolation procedure [7]. To characterize each gene, a pair of primers was developed using Oligo 5 (National Bioscience, Inc., Plymouth, MN, USA) based on the published cDNA sequence of GenBank (Tab.…”

Section: Dna Isolation Pcr and Sequencingmentioning

confidence: 99%

Association of INOS, TRAIL, TGF-β2, TGF-β3, and IgL genes with response to Salmonella enteritidis in poultry

Malek

Lamont

2003

Genet. Sel. Evol.

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-Several candidate genes were selected, based on their critical roles in the host's response to intracellular bacteria, to study the genetic control of the chicken response to Salmonella enteritidis (SE). The candidate genes were: inducible nitric oxide synthase (INOS), tumor necrosis factor related apoptosis inducing ligand (TRAIL), transforming growth factor β2 (TGF-β2), transforming growth factor β3 (TGF-β3), and immunoglobulin G light chain (IgL). Responses to pathogenic SE colonization or to SE vaccination were measured in the Iowa Salmonella response resource population (ISRRP). Outbred broiler sires and three diverse, highly inbred dam lines produced 508 F 1 progeny, which were evaluated as young chicks for either bacterial load isolated from spleen or cecum contents after pathogenic SE inoculation, or the circulating antibody level after SE vaccination. Fragments of each gene were sequenced from the founder lines of the resource population to identify genomic sequence variation. Single nucleotide polymorphisms (SNP) were identified, then PCR-RFLP techniques were developed to genotype the F 1 resource population. Linear mixed models were used for statistical analyses. Because the inbred dam lines always contributed one copy of the same allele, the heterozygous sire allele effects could be assessed in the F 1 generation. Association analyses revealed significant effects of the sire allele of TRAIL-StyI on the spleen (P < 0.07) and cecum (P < 0.0002) SE bacterial load. Significant effects (P < 0.04) were found on the cecum bacterial load for TGF-β3-BsrI. Varied and moderate association was found for SE vaccine antibody response for all genes. This is the first reported study on the association of SNP in INOS, TRAIL, TGF-β2, TGF-β3, and IgL with the chicken response to SE. Identification of candidate genes to improve the immune response may be useful for marker-assisted selection to enhance disease resistance.

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DNA fingerprints of chickens selected for high and low body weight for 31 generations

Cited by 82 publications

References 19 publications

Biodiversity of 52 chicken populations assessed by microsatellite typing of DNA pools

Biodiversity of 52 chicken populations assessed by microsatellite typing of DNA pools

Genetic Relationship Among Four Indian Breeds of Sheep Using RAPD-PCR

Association of INOS, TRAIL, TGF-β2, TGF-β3, and IgL genes with response to Salmonella enteritidis in poultry

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