DNA fingerprinting secondary transfer from different skin areas: Morphological and genetic studies

Zoppis, Silvia; Muciaccia, Barbara; D’Alessio, Alessio; Ziparo, Elio; Vecchiotti, Carla; Filippini, Antonio

doi:10.1016/j.fsigen.2014.03.005

Cited by 64 publications

(60 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…BExtrinsic^variables also were described such as different surface conditions and different manners of DNA application [19,20]. Besides nuclear DNA from keratinocytes [17], a recent study suggests Btrace DNA^to be derived from sebaceous glands [21]. All these studies investigated scenarios using more or less Bdry conditions^.…”

Section: Introductionmentioning

confidence: 99%

Everything clean? Transfer of DNA traces between textiles in the washtub

Kamphausen¹,

Fandel²,

Gutmann

et al. 2015

Int J Legal Med

View full text Add to dashboard Cite

Forensic genetic analysis of items possibly handled by a suspect or a victim is frequently inquired by the law enforcement authorities, since DNA left on touched objects can often be linked to an individual. Due to technical improvement, even poor traces, which seemed to be unsuitable for DNA analysis a few years ago, may be amplified successfully today. Yet, DNA can be transferred to a crime scene artificially or unintentionally without any primary contact between the individual and the object found at the crime scene, the so-called secondary transfer or indirect transfer in general. In this study, "secondary transfer" scenarios with cells and DNA of different origins under wet conditions were investigated. Transfer was simulated as either "washing by hand" in a washtub or as "machine laundry" in a washing machine. As expected, major differences were seen between blood stains and epithelial abrasions. DNA from blood donors could be detected clearly both on the donor and on the acceptor textile, regardless of washing method. Regarding epithelial abrasions, simulating worn clothes, after washing by hand, only little residual DNA was found, and partial profiles were displayed on the donor textile, while transfer to the acceptor textile occurred even less and not in noteworthy amount and quality. Single alleles could be found both on donor textiles and acceptor textiles after simulated machine wash, but no reliable DNA profile could be verified after laundry in machine. Therefore, a DNA transfer from one worn cloth (without blood stains) to another textile in the washing machine seems to be extremely unlikely.

show abstract

Section: Introductionmentioning

confidence: 99%

Everything clean? Transfer of DNA traces between textiles in the washtub

Kamphausen¹,

Fandel²,

Gutmann

et al. 2015

Int J Legal Med

View full text Add to dashboard Cite

show abstract

“…Once we know why and how touch DNA is transferred to objects, we will be able to better determine what pieces of evidence are suitable for collection and if touch DNA testing should be performed. Zoppis et al [21] determined that sebaceous fluid represents an important vector responsible for DNA transfer, therefore "touch DNA" secondary transfer is indeed possible depending on the specific sebaceous or non-sebaceous skin area previously touched. Cutaneous areas previously touched by our volunteers were not recorded in this experiment but could be something to take into consideration in future experiments.…”

Section: Discussionmentioning

confidence: 99%

Changes in Yield of Contact DNA over Time after a Physical Assault

Perez¹,

Ng²,

Oldt³

et al. 2014

J Forensic

View full text Add to dashboard Cite

“…Other studies examining the transfer of "touch" DNA in less controlled scenarios, considered more realistic examples of secondary transfer rates, have observed the transferred DNA as the major or only component to the profile on far fewer occasions, if at all:~2.8% in studies where the source of the transferred DNA was known (9-12);~1.4% in studies where the source of the transferred DNA was unknown (13,14). Several other studies allude to the detection of secondarily transferred DNA from known and/or unknown sources within the samples obtained, but provide insufficient information with respect to their presence forming a minor or major component of the profile, and in the case of foreign alleles, whether they originated from a single or multiple sources (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26).…”

Section: Sirmentioning

confidence: 99%