DNA fingerprinting in sugar beet (Beta vulgaris) — identification of double-haploid breeding lines

Schmidt, Thomas; Boblenz, K.; Metzlaff, Michael; Kaemmer, D.; Weising, Kurt; Kahl, Günter

doi:10.1007/bf00225001

Cited by 40 publications

(12 citation statements)

References 12 publications

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“…Moreover, these approaches could not assess the change(s), if any, at the DNA sequence level. During the last few years, molecular markers such as RFLPs (Breiman et al 1987;Chowdhury et al 1994, Shimron-Abarbanell andBreiman 1991;Shirzadegan et al 1991), RAPDs (Bohanec et al 1995;Brown et al 1993;Isabel et al 1993;Rani et al 1995) and oligonucleotide fingerprinting (Poulsen et al 1993;Schmidt et al 1993;Vosman et al 1992) have been found to be of tremendous utility in addressing fundamental and practical questions of plant tissue culture. In the study presented here, we have screened enhancedaxillary-branching-derived Eucalyptus tereticornis and E. camaldulensis plants by RFLP, RAPD, and oligonucleotide fingerprinting markers to assess their genetic fidelity, and to enquire into the applicability of the marker(s) for rapid appraisal of tissue-culture-derived eucalypt plants.…”

Section: Introductionmentioning

confidence: 98%

Genetic analysis of enhanced-axillary-branching-derived Eucalyptus tereticornis Smith and E. camaldulensis Dehn. plants

Rani

Raina²

1998

Plant Cell Reports

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In a culture method for enhanced axillary branching functional plants of Eucalyptus tereticornis and E. camaldulensis are efficiently regenerated. To assess the genetic integrity among the regenerants, we employed multiple analytical tools including cytochemical and molecular assays. The 2C DNA amounts were estimated in the meristematic zones of root and shoot tips of 250 micropropagated plants, collected at various cycles of tissue culture from multiplication to field transfer, and compared to the corresponding mother plants. The culture conditions did not induce amplification or deletion of DNA sequences, nor were there drastic change(s) in chromosome number, since all the micropropagated plants of E. tereticornis (1.2 pg) and E. camaldulensis (1.4 pg) maintained the same DNA amounts as the mother plant. Total DNA of 46 micropropagated and mother plants digested with eight restriction enzymes and hybridized to 13 nuclear, mitochondrial, and synthetic oligonucleotide DNA probes yielded 82 bands. Hybridization patterns indicated that the variation observed was minor. To further confirm the genetic fidelity, 12 arbitrary 10-base primers and six synthetic oligonucleotide sequences, successfully used to amplify genomic DNA from in vivo and in vitro materials, produced 133 fragments that were monomorphic across the plants tested. The present results demonstrate that enhanced-axillary-branching culture of mature trees could be utilized commercially for mass clonal propagation of these two important Eucalyptus species that have been recalcitrant to vegetative propagation. The results also provide novel insights into the genetic differences between E. tereticornis and E. camaldulensis.

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Section: Introductionmentioning

confidence: 98%

Genetic analysis of enhanced-axillary-branching-derived Eucalyptus tereticornis Smith and E. camaldulensis Dehn. plants

Rani

Raina²

1998

Plant Cell Reports

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“…SSRs are abundant within genomes and are present in both coding and noncoding regions. They are also usually characterized by a high degree of length polymorphism and, for this reason, they have proven to be extremely valuable tools for genome studies in many organisms and very powerful genetic markers that are often specific to single varieties or even individuals (Weising et al 1989;Schmidt et al 1993;Depeiges et al 1995;Schuler et al 1996;Knapik et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)4, (GT)7 and (TA)10 chromosome patterns

Bouilly

Chaves

Leitão

et al. 2008

J Genet

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Chromosome identification is essential in oyster genomic research. Fluorescence in situ hybridization (FISH) offers new opportunities for the identification of oyster chromosomes. It has been used to locate satellite DNAs, telomeres or ribosomal DNA sequences. However, regarding chromosome identification, no study has been conducted with simple sequence repeats (SSRs). FISH was used to probe the physical organization of three particular SSRs, (GGAT) 4 , (GT) 7 and (TA) 10 onto metaphase chromosomes of the Pacific oyster, Crassostrea gigas. Hybridization signals were observed in all the SSR probes, but the distribution and intensity of signals varied according to the oligonucleotide repeat. The intercalary, centromeric and telomeric bands were observed along the chromosomes, and for each particular repeat every chromosome pair presented a similar pattern, allowing karyotypic analysis with all the SSRs tested. Our study is the first in mollusks to show the application of SSR in situ hybridization for chromosome identification and karyotyping. This technique can be a useful tool for oyster comparative studies and to understand genome organization in different oyster taxa.

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“…Sugar beet (Beta vulgaris L.; 2n = 2x = 18) is a valuable model species for investigating the large scale organization of the nuclear genome because (i) the genome is relatively small with 758 Mbp (16), (ii) fluorescent in situ hybridization can accurately locate sequences along the metaphase chromosomes and within interphase nuclei (17), (iii) major classes of the repetitive DNA have been characterized including both satellite and retrotransposon sequences (18)(19)(20)(21)(22), and (iv) microsatellites are known to be highly abundant (23).…”

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confidence: 99%

The physical and genomic organization of microsatellites in sugar beet.

Schmidt

Heslop-Harrison

1996

Proc. Natl. Acad. Sci. U.S.A.

100

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Microsatellites, tandem arrays of short (2-5 bp) nucleotide motifs, are present in high numbers in most eukaryotic genomes. We have characterized the physical distribution of microsatellites on chromosomes of sugar beet (Beta vulgaris L.). Each microsatellite sequence shows a characteristic genomic distribution and motif-dependent dispersion, with site-specific amplification on one to seven pairs of centromeres or intercalary chromosomal regions and weaker, dispersed hybridization along chromosomes. Exclusion of some microsatellites from 18S-5.8S-25S rRNA gene sites, centromeres, and intercalary sites was observed. In-gel and in situ hybridization patterns are correlated, with highly repeated restriction fragments indicating major centromeric sites ofmicrosatellite arrays. The results have implications for genome evolution and the suitability of particular microsatellite markers for genetic mapping and genome analysis.Runs of repetitions of short sequence motifs 2-5 bp long, described as microsatellites or simple sequence repeats, are probably ubiquitous elements of eukaryotic genomes (1-3). They provide highly informative and polymorphic markers for genetics (4-6) or plant, fungal, and animal fingerprinting (7,8). Genetic mapping using microsatellites as markers involves amplification of repeat arrays using PCR with primers flanking the arrays. Primers are often chosen based on database searches or sequence data from cloned arrays (9-12), whichgive selective data about genomic distribution. However, little is known about the real chromosomal organization and physical localization of microsatellite motifs within plant genomes. The only microsatellite repeat mapped physically in plants, the polypurine motif (GAA)7, has been correlated with the positions of C-bands in barley (13). Some physical mapping in fish and primates (8, 14) using in situ hybridization shows clustering of microsatellites on some chromosomes. Recent data in mouse show that mapped microsatellites, mostly (CA)n repeats mapped by PCR polymorphisms, are distributed among autosomes in proportion to chromosome length, while the X chromosome shows a clear deficit of the microsatellites (15). Within chromosomes, the frequency of both large and small clusters with respect to meiotic crossovers slightly exceeded expectation.Sugar beet (Beta vulgaris L.; 2n = 2x = 18) is a valuable model species for investigating the large scale organization of the nuclear genome because (i) the genome is relatively small with 758 Mbp (16), (ii) fluorescent in situ hybridization can accurately locate sequences along the metaphase chromosomes and within interphase nuclei (17), (iii) major classes of the repetitive DNA have been characterized including both satellite and retrotransposon sequences (18)(19)(20)(21)(22), and (iv) microsatellites are known to be highly abundant (23).Here, we aimed to characterize the genomic distribution of microsatellite sequences in sugar beet. We used seven simple sequences representing a range of nucleotide motifs, many used for g...

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DNA fingerprinting in sugar beet (Beta vulgaris) — identification of double-haploid breeding lines

Cited by 40 publications

References 12 publications

Genetic analysis of enhanced-axillary-branching-derived Eucalyptus tereticornis Smith and E. camaldulensis Dehn. plants

Genetic analysis of enhanced-axillary-branching-derived Eucalyptus tereticornis Smith and E. camaldulensis Dehn. plants

Chromosomal organization of simple sequence repeats in the Pacific oyster (Crassostrea gigas): (GGAT)4, (GT)7 and (TA)10 chromosome patterns

The physical and genomic organization of microsatellites in sugar beet.

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