DNA fingerprinting in Indian major carps and tilapia by Bkm 2(8) and M13 probes

Abstract

DNA fingerprints were obtained in three species of commercially important freshwater fishes, Labeo rohita (Hamilton). Catla catla (Hamilton) and Oreachromis mossambicus (Peters), using Bkm 2(8) and M13 multilocus probes. Bkm 2(8) gave a higher number of bands when compared with M13. However, the number of bands obtained by each probe in O. mossambicus was similar. The higher band‐sharing coefficient observed in this species may be attributed to inbreeding as it arose from a small founder population. In L rohita and C. catla, the Bkm 2(8) detected similar DNA fingerprints when two enzymes Hinfi and Taqi were used. The M13 probe also gave similar fingerprints with three restriction enzymes (Hinfi, Taqi, Alui). Comparison of the DNA fingerprints obtained by Bkm 2(8) and Ml 3 showed that these two probes detected different alleles. The overall similarity of the DNA fingerprint patterns in L. rohita and C. catla may be due to their genetic closeness as indicated by their same chromosome number, C‐value and their ability to produce fertile hybrids. A similar argument also holds true for the Oreochromis species where interspecies hybridization results in fertile offspring.

A comparative account of the structure of the growth hormone encoding gene and genetic interrelationship in six species of the genus Labeo

“…Cloning of chromosomal growth hormone gene from different Labeo species Genomic DNA was prepared from the stored blood samples by proteinase-K digestion and phenol extraction (Majumdar et al 1997). Oligonucleotide primers were designed from the sequenced L. rohita GH gene obtained by us (Genbank Accession AF 418921), including both 5 0 and 3 0 untranslated regions encompassing the poly-A signal.…”

Fish genetics and conservation research in India: status and perspectives

“…In marine fishes, speciesspecific allozyme markers were developed to resolve the taxonomic ambiguity in marine catfish species such as Tachysurus maculatus and T. subrostratus. DNA fingerprints of Indian major carps were obtained using banded Krait minor satellite 3 [BKM(3)] and M13 multilocus probes (Majumdar et al 1997) and using RAPD markers (Barman et al 2003;Das et al 2005b). Menezes et al (2002) identified diagnostic loci suitable for stock delineation and species identification among five nemipterid fish species from the Indian coast using allozyme analysis.…”

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“…In hybridization study, growth comparison of the reciprocal hybrids is important because they are seldom the same (Tave, Jayaprakash, & Smitherman, 2007). Various genetic studies have been carried out in L. rohita, including DNA fingerprinting by using a sex specific satelite DNA [Bkm 2(8)] and a minisatelite DNA [M13] probes, (Majumdar, Ravinder, & Nasaruddin, 1997), detection of hybrids in the three Indian major carp (catla, rohu and mrigal) using ribosomal DNA RFLP with Xenopus laevis rDNA as a probe (Padhi & Mandal, 1997), RAPD analysis (Barman et al, 2003;Islam & Alam, 2004) and allozyme analysis (Alam, Akanda, Khan, & Alam, 2002;Khan, Rahman, A1am, & Bhuiyan, 2006). Previous studies involving allozyme and RAPD analysis revealed relatively low levels of intra and inter-population genetic variation in the three river populations of rohu and catla (Alam et al, 2002;Islam & Alam, 2004;Islam, Ahmed, Azam, & Alam, 2005) and a wide spread hybridization practices in the hatchery stocks among the three Indian major carp species (Simonsen, Hansen, Mensberg, Serder, & Alam, 2005).…”