DNA fingerprinting and molecular diversity analysis of brinjal (Solanum melongena L.) cultivars using RAPD markers

Sultana, Sharmin; Islam, Mohammad Zahidul; Hoque,

doi:10.5455/jabet.d1

Cited by 6 publications

(8 citation statements)

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“…Eggplants (Solanum) represent economically important vegetables widely consumed in the world (Sultana et al 2018). They belong to the family Solanaceae and consist of about 1500 morphologically diverse species (Aguoru et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of exotic and local eggplants (Solanum spp.) cultivated in Côte d’Ivoire based on ISSR markers

et al. 2020

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Abstract. Konan NO, Akaffou MA, Kouadio L, Akaffou DS, Mergeai G. 2020. Genetic diversity of exotic and local eggplants (Solanum spp.) cultivated in Côte d’Ivoire based on ISSR markers. Biodiversitas 21: 3651-3658. Eggplants are important vegetables in Côte d'Ivoire. Despite an increase in the cultivation of exotic genotypes and their progressive dominance over local cultivars, which could represent a threat of genetic erosion, there are not enough molecular data available on the genetic diversity of the eggplants currently cultivated in this country. This study is a contribution to the knowledge of the genetic diversity of the current eggplant cultivars grown in Côte d’Ivoire. It aimed to assess the genetic variation of nine exotic and local eggplants commonly cultivated in Côte d'Ivoire, using five inter-simple sequence repeat (ISSR) primers. The results revealed for the exotic cultivars 42 ISSR loci of which 31 were polymorphic (73.81 %), and for local cultivars 51 loci of which 49 (96.08%) were polymorphic. The Nei’s genetic diversity and Shannon’s information index of local cultivars (He = 0.3333 and I = 0.4863) were higher than those of exotic cultivars (He = 0.2000 and I = 0.2971). The Gst value and the AMOVA analysis found more than 70% of the total genetic diversity within the groups. The Jaccard’s dissimilarity coefficients between the cultivars, ranged from 0.324 to 0.966 with an average of 0.741. The clustering of genotypes with the neighbor-joining unrooted tree and PCoA analysis differentiated three main clusters with exotic cultivars grouped in cluster I and local cultivars in cluster II and III. In view of these results, the local eggplants had higher genetic diversity and constitute an interesting germplasm which needs to be conserved for potential breeding programs.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity of exotic and local eggplants (Solanum spp.) cultivated in Côte d’Ivoire based on ISSR markers

et al. 2020

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“…The PCR based assays are also depending on the sequencing or randomly amplified patterns analysis. Random PCRs methods are simple and cost-effective and easily can apply to many microorganisms (13).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of (GTG) 5-PCR for Genotyping of Klebsiella pneumonia Strains Isolated from Patients with Urinary Tract Infections

Ranjbar

Afshar

2020

ijph

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Background: Klebsiella pneumonia is one of the common causes of pneumonia and bacteremia in intensive care patients. The present study was aimed to determine the capability of (GTG) 5-PCR assay for molecular typing of K. pneumonia strains isolated from patients with urinary tract infections. Methods: In this descriptive-sectional study, K. pneumoniae strains were collected from hospitalized patients with urinary tract infection in Baqiyatallah Hospital, Tehran, Iran during 2017-2018. Isolates were identified by conventional microbiological tests. Bacterial DNA was extracted using boiling method and (GTG) 5-PCR assay was used for subtyping of the isolates. For clustering of isolates, dendrogram was generated according to the unweighted pair group method with arithmetic (UPGMA). Results: Overall, 88 K. pneumoniae isolates were isolated and subjected to the molecular typing study. The (GTG) 5–PCR assay was able to differentiate the K. pneumoniae strains into 9 clusters including G1-G9. Genotype clusters G4 and G9 consist of highest (26) and lowest (1) number isolate, respectively. Conclusion: The K. pneumonia strains isolated under the study belonged to various clones and the (GTG) 5-PCR assay as simple and rapid method can be a powerful tool for molecular typing of K. pneumoniae strains.

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“…It contains different species and varieties which are distinguished in particular by the color, size and shape of the fruits (Furini & Wunder 2004). It is of great economic importance because several species are cultivated and consumed in the world (Sultana et al 2018). The most cultivated species are Solanum macrocarpon, Solanum melongena and Solanum aethiopicum (Wei et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Investigating Eggplant Fruit (Solanum spp.) Cultivated in Côte d’Ivoire for Their Physicochemical and Antioxidant Characteristics

2020

FSQM

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Eggplant is one of the most commonly consumed vegetables in Côte d'Ivoire. For this purpose, the nutrient constituents of eggplant varieties (Niguema, N'drowa, Aliona, Bello, Kotobi, Italy, Tiger, Kalenda and Gnangnan) were evaluated by standard procedures. The proximate composition in dry weight basis was significantly (P < 0.05) varied and ranged: dry matter 86.72-93.29%, crude protein 9.94-22.11%, crude fat 5.07-20.43%, crude fiber 23.15-50.53%, crude ash 5.14-10.09%, carbohydrate 12.41-39.22%, and energy value 211.53-320.94 kcal/100g. Gnangnan had the highest content in fat. Kalenda had the highest protein content, N'drowa had the highest fiber content, Bello had the highest content in carbohydrate, while Tiger presented the highest content in both ash and energy values. The phenolic content ranges from 618.28 to 977.35 mg/100g while the vitamin C ranges from 92.88 to 150.59 mg/100g in dry weight basis. Italy had the highest content in phenolic while Kalenda had the highest content in vitamin C. Interrelationships between the parameters analysed and the different eggplant varieties were investigated by principal component analysis (PCA). PCA revealed differences between the eggplant varieties and classified them into three clusters on the basis of the measured parameters: Cluster 1 (Gnangnan), Cluster 2 (Niguema, N'drowa, Italy and Aliona) and Cluster 3 (Bello, Kalenda, Kotobi and Tiger) This study is a contribution to the knowledge of the nutritional composition of eggplant cultivated in Côte d'Ivoire. In view of these results, eggplant can help to reduce the nutrition-related disorders in Africa.

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DNA fingerprinting and molecular diversity analysis of brinjal (Solanum melongena L.) cultivars using RAPD markers

Cited by 6 publications

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Genetic diversity of exotic and local eggplants (Solanum spp.) cultivated in Côte d’Ivoire based on ISSR markers

Genetic diversity of exotic and local eggplants (Solanum spp.) cultivated in Côte d’Ivoire based on ISSR markers

Evaluation of (GTG) 5-PCR for Genotyping of Klebsiella pneumonia Strains Isolated from Patients with Urinary Tract Infections

Investigating Eggplant Fruit (Solanum spp.) Cultivated in Côte d’Ivoire for Their Physicochemical and Antioxidant Characteristics

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