DNA fingerprinting and genetic diversity analysis of late-bolting radish cultivars with RAPD, ISSR and SRAP markers

Liu, Liwang; Zhao, Lin; Gong, Yiqin; Wang, Mingxia; Chen, Liming; Yang, Juan; Wang, Yan; Yu, Fan-Min; Wang, Longzhi

doi:10.1016/j.scienta.2007.12.011

Cited by 93 publications

(51 citation statements)

References 25 publications

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“…These results indicate a great genetic diversity among Brazilian cultivars which can be explained by the high polymorphism found in both the species and the crop. In a study with RAPD in radish populations, 82% polymorphism was detected (MADHOU et al, 2005), 78.2% in the germplasm bank of Pakistan (RABBANI et al, 1998) and 85.4% between the radish cultivars (LIU et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

“…Hu and Quiros (1991) identified fourteen broccoli and twelve cauliflower cultivars using four RAPD primers, and stated that two or three primers are enough to differentiate between each of the cultivars studied. Liu et al (2008), studying 35 radish cultivars found it possible to distinguish them with five sets of three primers.…”

Section: Resultsmentioning

confidence: 99%

“…Some of the amplified products are shown in Figure 6. Using 22 ISSR primers to distinguish 35 cultivars, Liu et al (2008) obtained 250 bands, with 85.2% polymorphism, an average of 7 polymorphic bands per primer. They considered that the minimum number of primers The similarity index calculated by the Jaccard coefficient was 0.45, lower than that calculated by RAPD (0.71).…”

Section: Resultsmentioning

confidence: 99%

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Molecular characterisation of radish cultivars

Cruz¹,

Nery²,

Pinho³

et al. 2014

Rev. Ciênc. Agron.

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-The radish has been gaining importance in the Brazilian market due to its use as a green manure and cover crop, and also due to its potential for the production of biodiesel, however there are only two registered cultivars, which are morphologically very similar. Determination of genetic purity is a prerequisite in marketing seeds as it ensures uniformity of the crop and is important in breeding programs. The aim of this work therefore was to evaluate the similarity and genotype patterns which permit differentiation of the commercial radish cultivars IPR 116 and CATI AL-1000. In order to do this, isoenzyme electrophoretic patterns were analysed in dry seeds, soaked seeds, seedling leaves and young leaves; the isoenzymes used being: superoxide dismutase, catalase, esterase, glutamate-oxalocetate, malate dehydrogenase and isocitrate lyase. Thirtyseven RAPD primers and 10 ISSR primers in leaves of the CATI AL-1000 and IPR-116 cultivars were analysed. Among the isoenzymes under study, the most polymorphic were glutamate oxalocetate, malate dehydrogenase, esterase and superoxide dismutase, with the superoxide dismutase system giving the best characterisation for all stages of development. The catalase isoenzyme system did not make it possible to differentiate between cultivars at any stage of development, and isocitrate lyase was not revealed by the protocol used. In analysis of the markers, 27 RAPD primers and eight ISSR primers showed polymorphism. The results indicate that it is possible to determine reliable descriptors based on isoenzymes at different stages of development of the radish and with the use of RAPD and ISSR primers. RESUMO -O nabo-forrageiro tem ganhado importância no mercado brasileiro como adubo verde, planta de cobertura e pelo potencial para produção de biodiesel, no entanto estão registradas apenas duas cultivares que são morfologicamente muito semelhantes. A determinação da pureza genética é um dos requisitos para comercialização de sementes, pois garante a uniformidade de plantio, e é importante para os programas de melhoramento. Por tanto, objetivou-se com este trabalho avaliar a similaridade e padrões genotípicos que permitam diferenciar as cultivares comerciais de nabo-forrageiro 'IPR 116' e 'CATI AL-1000'. Para isso, foi analisado o padrão eletroforético de isoenzimas em sementes secas, sementes embebidas, folhas de plântulas e folhas jovens. As isoenzimas usadas foram: superóxido dismutase, catalase, esterase, glutamato -oxilacetato, malato desidrogenase e isocitrato liase. Foram analisados 37 primers RAPD e 10 primers ISSR em folhas das cultivares 'CATI AL-1000' e 'IPR-116'. Entre as isoenzimas estudadas, as mais polimórficas foram glutamato -oxilacetato, malato desidrogenase, esterase e superóxido dismutase, tendo o sistema superóxido dismutase realizado a melhor caracterização para todos os estádios de desenvolvimento. O sistema isoenzimático catalase não permitiu distinguir as cultivares em nenhum estádio de desenvolvimento e a isocitrato liase não revelou com o protocolo u...

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular characterisation of radish cultivars

Cruz¹,

Nery²,

Pinho³

et al. 2014

Rev. Ciênc. Agron.

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“…Depending on the strategy adopted, all these can in turn make RAPD a useful technique in plant cultivar and variety identification to varying degrees as shown by recent studies using the manual cultivar identification diagram (MCID) strategy in different crops Zhao et al, 2011;Wang et al, 2011;Li et al, 2011). RAPD markers have also been successfully applied in determining genetic relationships in vegetables such as Capsicum (Bhadragoudar and Patil, 2011), broccoli (Lu et al, 2009), radish (Liu et al, 2008), and curcurbits (Dey et al, 2006;Sureja et al, 2006;Verma et al, 2007), among other crops.…”

Section: Introductionmentioning

confidence: 99%

A novel and efficient strategy for practical identification of tomato (Solanum lycopersicon) varieties using modified RAPD fingerprints

Korir

Li²,

Leng³

et al. 2013

Genet. Mol. Res.

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ABSTRACT.Tomato breeding and variety development have led to the generation of a large number of varieties in many countries worldwide. This has created a growing and urgent need for an improved strategy for genotyping and identification since the traditional methods based on phenotype are growing unreliable. DNA markers could provide distinct benefits in tomato variety identification; however, DNA fingerprint analyses have not made DNA marker data readily usable for identification of varieties in tomato and other crops. A manual cultivar and/or variety identification diagram (MCID) strategy has been developed and has been found to make DNA markers more usable for the identification of genotyped plant individuals. We adopted this strategy, using modified RAPD markers to identify 42 tomato varieties from different geographical origins and seed merchants. All of the varieties were clearly separated and individually identified by reproducible fingerprints of only 6 RAPD primers. The tomato MCID that is generated is usable for the identification of any two or more tomato varieties. In addition, fewer primers can be used to make a distinction between varieties using this approach, since the selected fingerprints from each primer are used after they have been generated. The information in this 1817 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (2): 1816-1828 (2013 An efficient strategy for identification of tomato varieties first version of the tomato MCID can be enriched through identification and incorporation of more varieties and adaptation to other molecular markers in order to provide a more comprehensive tomato variety identification service for the horticultural industry.

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“…Thus, several techniques based on DNA analysis were used, such as random amplified polymorphic DNA (RAPD) (Román, Rubiales, Torres, Cubero, & Satovic, 2001), inter simple sequence repeat (ISSR) (Román et al, 2002;Westwood & Fagg, 2004), amplified fragment length polymorphism (AFLP) (Gagne et al, 2000), and simple sequence repeat (SSR) markers (Pineda-Martos et al, 2014). Moreover, Sequence-Related Amplified Polymorphism (SRAP markers) is a simple and efficient molecular marker system, more reproducible than RAPDs ISSR, and SSR markers Liu et al, 2008;Liu et al, 2006) and easier to assay than AFLPs (Ferriol et al, 2003). This technique is based on open reading frames (ORFs) using two primers (forward and reverse) for amplification.…”

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confidence: 99%

Genetic Diversity of Moroccan Orobanche crenata Populations Revealed by Sequence-Related Amplified Polymorphism Markers

Ennami¹,

Briache²,

Mbasani-Mansi³

et al. 2017

JAS

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Orobanche crenata Forsk. is a root holoparasitic plant that affects legume species in Mediterranean basin especially in Northern Africa. This parasitic weed is particularly problematic in faba bean (Vicia faba L.) and lentil (Lens culinaris Medik.) fields. In Morocco, development of legume resistant/tolerant genotypes is considered the most economical and ecological control strategy against O. crenata. Efficient selection of resistant/tolerant cultivars requires prerequisite knowledge of the genetic diversity of the parasite. Thus, the present study focused on the assessment of the genetic diversity among and within Moroccan O. crenata populations, growing in faba bean fields, using Sequence-Related Amplified Polymorphism markers (SRAP). This marker system proved to be a powerful and an efficient tool for the evaluation of the genetic diversity among O. crenata populations. In fact, a total of 101 markers were identified and used for the Analysis of Molecular Variance (AMOVA), among which 98 bands were polymorphic (97.02%), indicating considerable genetic variation of these O. crenata populations. However, at population level, low level of polymorphic loci was observed with a percentage ranging between 41.58% and 67.33%. The Jaccard's similarity coefficient and Principal Coordinate Analysis (PCoA) showed a clear differentiation among O. crenata samples according to the geographical origin of each population. AMOVA analysis revealed also a large extent of variation among O. crenata populations (60%; p < 0.010). Our outputs on molecular genetics of O. crenata combined with future epidemiological studies of these populations should clarify occurrence of O. crenata pathotypes and thereby validate the relevance of using multisite screening trials during breeding programs.

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DNA fingerprinting and genetic diversity analysis of late-bolting radish cultivars with RAPD, ISSR and SRAP markers

Cited by 93 publications

References 25 publications

Molecular characterisation of radish cultivars

Molecular characterisation of radish cultivars

A novel and efficient strategy for practical identification of tomato (Solanum lycopersicon) varieties using modified RAPD fingerprints

Genetic Diversity of Moroccan Orobanche crenata Populations Revealed by Sequence-Related Amplified Polymorphism Markers

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