DNA Fingerprinting and Cloning of Hypervariable Minisatellite Repeats in Salmonids

Leung, Fcc; Welt, M; Quesenberry, RD; Shen, XZ

doi:10.1139/f94-312

Cited by 4 publications

(1 citation statement)

References 24 publications

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“…Back-assignment of salmonid progeny to parents has been done in the past, but these studies did not have the advantage of having DNA samples of all adults that contributed to the population (Stevens et al 1993;Leung et al 1994;Hudy et al 2010;Vollestad et al 2012;Kanno et al 2014). Here, we successfully assigned 87.9% (n = 1,279) of the juveniles to a single parent pair.…”

Section: Source and Introduction Sites Yearmentioning

confidence: 99%

Performance of Juvenile Cutthroat Trout Translocated as Embryos from Five Populations into a Common Habitat

Andrews¹,

Shepard

Litt³

et al. 2016

N American J Fish Manag

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The distributions of most native trout species in western North America have been severely reduced, and conservation of many of these species will require translocation into vacant habitats following removal of nonnative species. A critical question managers have is “Does it matter which donor sources are used for these translocations?” We present a case study that addressed this question for a large native trout translocation project in Montana. We introduced embryos from five source populations of Westslope Cutthroat Trout Oncorhynchus clarkii lewisi to a large, fishless watershed in Montana following removal of nonnative fish with piscicides. Source populations providing embryos for translocations were three nearby (<120 km) wild populations, the state of Montana's captive Westslope Cutthroat Trout hatchery conservation population (initiated 32 years ago using fish from wild populations located >350 km from the translocation site), and a population in captivity for one generation comprised of individuals from the three wild populations used as single sources for this project, which were variably crossed (59% within populations and 41% between populations) to provide embryos. We used remote‐site incubators at six different sites to introduce approximately 35,000 embryos from 400 genotyped parents. We later resampled and genotyped 1,450 of these individuals at age 1 and age 2. Juvenile survival for the more genetically diverse Montana Westslope Cutthroat Trout conservation population was twice as high as for other source populations, even though these other source populations were geographically closer to the translocation site than populations used to make the Montana Westslope Cutthroat Trout conservation population. Body weight for progeny from the two captive populations was higher than for progeny from wild source populations, and some differences were observed in body condition among source populations. Continued monitoring over several generations will be necessary to determine the eventual contributions of each source population and the relevance of these initial findings. Received October 9, 2015; accepted February 27, 2016 Published online July 20, 2016

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Section: Source and Introduction Sites Yearmentioning

confidence: 99%

Performance of Juvenile Cutthroat Trout Translocated as Embryos from Five Populations into a Common Habitat

Andrews¹,

Shepard

Litt³

et al. 2016

N American J Fish Manag

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New applications of low-C0t DNA as a DNA fingerprint probe

Leung

1999

Electrophoresis

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New applications of low‐C0t DNA are reported as probes for genetic identification and genome characterization. These fast and intermediately reannealing fractions have sometimes either been discarded in genomic library construction to enhance the probability of finding single copy genes, or they are used as resources for identifying individual repetitive sequences. In addition, they are used as blockers to enhance hybridization signals. C0t‐1 DNA serves as a probe for DNA fingerprinting of human yeast artificial chromosomes. We have isolated low‐C0t DNA from bacteria, fungus, plant, mussel, chicken, rat and fish from the sheared genomic DNA of the respective species. Low‐C0t DNA is labeled to generate DNA fingerprints and for in situ hybridization. Individual specific DNA fingerprint profiles are observed and species‐specific DNA fragments can be identified in bacteria, fungus, plants (Ginseng and Amaranthus) and mussel. When low‐C0t DNA probes from rat, chicken and fish were employed, only smear profiles and no distinct DNA banding patterns were evident. In these species, individual clones can be used as a probe for DNA fingerprinting containing repetitive sequences after subcloning. The advantage of this approach is to quickly develop a useful probe for DNA fingerprinting for genetic identification and analysis without sequencing knowledge a priori. This represents an innovative approach to the use of these repetitive components of the genome.

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Experimental Design and Sampling Strategies for Mixed-Stock Analysis

Fabrizio

2005

Stock Identification Methods

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DNA Fingerprinting and Cloning of Hypervariable Minisatellite Repeats in Salmonids

Cited by 4 publications

References 24 publications

Performance of Juvenile Cutthroat Trout Translocated as Embryos from Five Populations into a Common Habitat

Performance of Juvenile Cutthroat Trout Translocated as Embryos from Five Populations into a Common Habitat

New applications of low-C0t DNA as a DNA fingerprint probe

Experimental Design and Sampling Strategies for Mixed-Stock Analysis

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