DNA extraction and PCR amplification method suitable for fresh, herbarium-stored, lichenized, and other fungi

Cubero, Oscar F.; Crespo, Ana; Fatehi, Jamshid; Bridge, P. D.

doi:10.1007/bf01084401

Cited by 362 publications

(232 citation statements)

References 11 publications

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“…The material was first frozen in liquid nitrogen and then pulverized with polypropylene pestles. The DNA was extracted according to the protocol of Cubero et al (1999). Molecular sequence data from Cheiromycina samples were generated for the nuclear large and partial nuclear small ribosomal subunits (28S and 18S, respectively) and for the mitochondrial small ribosomal subunit (12S).…”

Section: Methodsmentioning

confidence: 99%

Molecular analyses uncover the phylogenetic placement of the lichenized hyphomycetous genusCheiromycina

et al. 2017

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The genus Cheiromycina is one of the few genera of lichenized hyphomycetes for which no sexual reproductive stages have been observed. The genus includes species from boreal to temperate regions of the Northern Hemisphere where it is found growing on bark or wood. Congeners in Cheiromycina are characterized by a noncorticate thallus, nearly immersed in the substrate and presenting powdery unpigmented sporodochia, and containing chlorococcoid photobionts. The relationships of members of Cheiromycina with other fungi are not known. Here we inferred the phylogenetic placement of Cheiromycina using three loci (nuSSU, nuLSU, and mtSSU) representing C. flabelliformis, the type species for the genus, C. petri, and C. reimeri. Our results revealed that the genus Cheiromycina is found within the family Malmideaceae (Lecanorales) where members formed a monophyletic clade sister to the genera Savoronala and Malmidea. This phylogenetic placement and the relationships of Cheiromycina with other lichenized hyphomycetous taxa are here discussed.

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Section: Methodsmentioning

confidence: 99%

Molecular analyses uncover the phylogenetic placement of the lichenized hyphomycetous genusCheiromycina

et al. 2017

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“…Total genomic DNA was extracted from the ground up samples using a polyvinyl pyrolidine/cetrimide method Cubero et al, 1999 [13] PCR amplification of samples was undertaken using the primer GanET with a universal primer, ITS3 White et al, 1990 [6]. The reactions were undertaken in 50μl volumes in a reaction mixture consisting of; 4μl dNTPs, 2μl DNA, 3μl…”

Section: Samplingmentioning

confidence: 99%

Following basal stem rot in young oil palm plantings

Panchal

Bridge

2005

Mycopathologia

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“…The remnants were then further inspected under both the dissecting and light microscopes in order to avoid contamination by other fungi. Genomic DNA isolation was performed by means of a modified version of the CTAB method (Cubero et al 1999). Internal transcribed spacer (ITS) and partial nuLSU rDNA regions were selected for amplification, using primers ITS1F (Gardes and Bruns 1993), ITS1LM (Myllys et al 1999) and ITS4 (White et al 1990) for ITS, and LR0R (Rehner and Samuels 1994) and LR5 (Vilgalys and Hester 1990) for nuLSU.…”

Section: Dna Extraction Pcr Amplification and Dna Sequencingmentioning

confidence: 99%

Unravelling the diversity of European Caliciopsis (Coryneliaceae, Ascomycota): Caliciopsis valentina sp. nov. and C. beckhausii comb. nov., with a worldwide key to Caliciopsis

Garrido‐Benavent

Pérez‐Ortega

2015

Mycol Progress

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Unravelling the diversity of EuropeanPostprint del artículo publicado en: Mycological Progress 14: 1-11 (2015) AbstractThe new species Caliciopsis valentina from the eastern Iberian Peninsula is characterized morphological, anatomical and molecularly. The occurrence of C. subcorticalis (Cooke & Ellis) Fitzp. in Europe is discussed. Based on the revision of fresh and herbarium specimens we propose the new combination Caliciopsis beckhausii with a neotype selected for this taxon. New molecular data (nrITS and nuLSU) are used in combination of available sequences to build a preliminary phylogenetic hypothesis for this genus. We point some previously overlooked colour-reaction tests as relevant for the systematics of the group. Finally, an updated key for all known Caliciopsis species is provided.

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DNA extraction and PCR amplification method suitable for fresh, herbarium-stored, lichenized, and other fungi

Cited by 362 publications

References 11 publications

Molecular analyses uncover the phylogenetic placement of the lichenized hyphomycetous genusCheiromycina

Molecular analyses uncover the phylogenetic placement of the lichenized hyphomycetous genusCheiromycina

Following basal stem rot in young oil palm plantings

Unravelling the diversity of European Caliciopsis (Coryneliaceae, Ascomycota): Caliciopsis valentina sp. nov. and C. beckhausii comb. nov., with a worldwide key to Caliciopsis

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