DNA Extracted with Optimized Protocols Can Be Genotyped to Reconstruct the Varietal Composition of Monovarietal Wines

Bigliazzi, Jacopo; Scali, Monica; Elisa, Paolucci; Cresti, Mauro; Vignani, Rita

doi:10.5344/ajev.2012.12014

Cited by 29 publications

(39 citation statements)

References 22 publications

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“…Even though it is well known that wine fermentation yields DNA highly degraded (Garcia-Beneytez, Moreno-Arribas, Borrego, Polo, & Ibáñez, 2002), it is also true that must samples still contain high fragment genomic DNA, which enables it to be successfully used in marker systems that target large fragments, such as SSR markers (Baleiras-Couto & Eiras-Dias, 2006;Bigliazzi et al 2012;Pereira et al, 2012;Rodríguez-Plaza et al, 2006). The fragment size used in this assay is the biggest specific fragment reported so far in must samples, and it is interesting because it allows the discrimination of multiple events in one unique reaction, overcoming the potential obtained through SSR analysis which detects repeat variation.…”

Section: Resultsmentioning

confidence: 99%

“…Another drawback in the application of SSR markers, in such sample type, is related with DNA degradation, result of alcoholic fermentation process. However, several DNA extraction protocols have been improved and have managed to increase both the yield and the quality of the extracted DNA (e.g., Baleiras-Couto & Eiras-Dias, 2006;Bigliazzi, Scali, Paolucci, Cresti, & Vignani, 2012;Boccacci, Akkak, Marinoni, Gerbi, & Schneider, 2012;Nakamura, Haraguchi, Mitani, & Ohtsubo, 2007;Pereira, Guedes-Pinto, & Martins-Lopes, 2011;Savazzini & Martinelli, 2006). The use of small molecular markers, such as, Single Nucleotide Polymorphisms (SNPs) could be a way to overcome the natural DNA degradation found in such samples.…”

Section: Introductionmentioning

confidence: 99%

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High Resolution Melting (HRM) applied to wine authenticity

et al. 2017

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Wine authenticity methods are in increasing demand mainly in Denomination of Origin designations. The DNA-based methodologies are a reliable means of tracking food/wine varietal composition. The main aim of this work was the study of High Resolution Melting (HRM) application as a screening method for must and wine authenticity. Three sample types (leaf, must and wine) were used to validate the three developed HRM assays (Vv1-705bp; Vv2-375bp; and Vv3-119bp). The Vv1 HRM assay was only successful when applied to leaf and must samples. The Vv2 HRM assay successfully amplified all sample types, allowing genotype discrimination based on melting temperature values. The smallest amplicon, Vv3, produced a coincident melting curve shape in all sample types (leaf and wine) with corresponding genotypes. This study presents sensitive, rapid and efficient HRM assays applied for the first time to wine samples suitable for wine authenticity purposes.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High Resolution Melting (HRM) applied to wine authenticity

et al. 2017

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“…Nevertheless, due to recent technological advancements, it is possible to detect and discriminate the grapevine variety present in such sample types [4,8,75]. In an authenticity system, it is crucial to establish a framework concerning the most suitable sampling and storage conditions regarding the subsequent steps and the final defined purpose (Figure 3).…”

Section: Dna Fingerprinting For Varietal Identificationmentioning

confidence: 99%

From the Field to the Bottle—An Integrated Strategy for Wine Authenticity

et al. 2018

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The wine sector is one of the most economically important agro-food businesses. The wine market value is largely associated to terroir, in some cases resulting in highly expensive wines that attract fraudulent practices. The existent wine traceability system has some limitations that can be overcome with the development of new technological approaches that can tackle this problem with several means. This review aims to call attention to the problem and to present several strategies that can assure a more reliable and authentic wine system, identifying existent technologies developed for the sector, which can be incorporated into the current traceability system.

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“…[22,26,[90][91][92][93][94][95][96][97][98] Apple (Malus Â domestica Borkh.) [99][100][101][102][103][104][105][106][107][108][109][110][111] Derivatives Wine [45,[112][113][114][115][116] Olive oil [46,58,[117][118][119][120][121][122][123][124][125] Article searches were performed using the three most popular sources of scientific information: Scopus, Web of Science, and Google Scholar, while PubMed was excluded from the queried datasets because it focuses mainly on medicine and biomedical sciences and also because Google scholar already includes its index [126]. A total of 90 articles based on SSR genotyping analysis were selected from the international literature in the last 15 years, covering all the plant species/ food products taken as reference list.…”

Section: Crops Referencesmentioning

confidence: 99%

Critical Aspects on the Use of Microsatellite Markers for Assessing Genetic Identity of Crop Plant Varieties and Authenticity of their Food Derivatives

Palumbo¹,

Barcaccia²

2018

Rediscovery of Landraces as a Resource for the Future

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A total of 90 original articles concerning the varietal characterization and identification by means of SSR analysis of the five most economically relevant crops in Italy (i.e., Olea europaea L., Solanum lycopersicum L., Vitis vinifera L., Triticum spp. and Malus Â domestica Borkh.) have been selected and reviewed. Since the genetic traceability of processed products may result more complex, wine and olive oil have been considered too. Specifically, this chapter deals with three main aspects: (i) the criteria adopted for the selection of the most appropriate number, type, and distribution of SSR marker loci to be employed for varietal genotyping, (ii) the use of genetic statistics and parameters for the evaluation of the discriminant ability and applicability of SSR marker loci, and (iii) how to make different experimental works on the same species that are standardized, reliable, and comparable. What emerges from the studies reviewed here is a lack of wider consensus among the authors regarding the strategy to design and to adopt for genotyping plant varieties with SSR markers. This finding highlights the urgent need to establish a common procedure, especially for characterizing and preserving landraces, and for supporting its rediscovery and valorization locally.

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DNA Extracted with Optimized Protocols Can Be Genotyped to Reconstruct the Varietal Composition of Monovarietal Wines

Cited by 29 publications

References 22 publications

High Resolution Melting (HRM) applied to wine authenticity

High Resolution Melting (HRM) applied to wine authenticity

From the Field to the Bottle—An Integrated Strategy for Wine Authenticity

Critical Aspects on the Use of Microsatellite Markers for Assessing Genetic Identity of Crop Plant Varieties and Authenticity of their Food Derivatives

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