DNA encapsulated magnesium and manganous phosphate nanoparticles: potential non-viral vectors for gene delivery

Bhakta, Gajadhar; Mitra, Susmita; Maitra, Amarnath

doi:10.1016/j.biomaterials.2004.06.039

Cited by 66 publications

(53 citation statements)

References 23 publications

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“…When the aqueous solution of pectin was dropped into divalent cation solution (CaCl 2 , MgCl 2 or MnCl 2 ), pectinate particles were instantaneously produced by ionotropic gelation. In this process, intermolec- (28)(29). To investigate the conditions for micro/nanoparticle formation, systems that were able to produce gel aggregates were identified depending on the concentration of pectin and divalent cations.…”

Section: Preparation Of Pectinate Microparticlesmentioning

confidence: 99%

“…Mg-pectinate nanoparticles showed higher transfection efficiency than Ca-pectinate. It has been reported that the plasmid DNA encapsulated in either calcium phosphate or magnesium phosphate nanoparticles could be successfully used as a non-viral vector (28)(29). The higher transfection efficiency of Mg-pectinate nanoparticles might be due to lower complexation reaction between magnesium ion and DNA including other biopolymers present in the system.…”

Section: In Vitro Gene Expressionmentioning

confidence: 99%

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Development and Characterization of Pectinate Micro/Nanoparticles for Gene Delivery

et al. 2008

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Abstract. The aim of this study was to investigate the possibility of using pectinate micro/nanoparticles as gene delivery systems. Pectinate micro/nanoparticles were produced by ionotropic gelation. Various factors were studied for their effects on the preparation of pectinate micro/nanoparticles: the pH of the pectin solution, the ratio of pectin to the cation, the concentration of pectin and the cation, and the type of cation (calcium ions, magnesium ions and manganese ions). After the preparation, the size and charge of the pectin micro/nanoparticles and their DNA incorporation efficiency were evaluated. The results showed that the particle sizes decreased with the decreased concentrations of pectin and cation. The type of cations affected the particle size. Sizes of calcium pectinate particles were larger than those of magnesium pectinate and manganese pectinate particles. The DNA loading efficiency showed that Capectinate nanoparticles could entrap DNA up to 0.05 mg when the weight ratio of pectin:CaCl 2 :DNA was 0.2:1:0.05. However, Mg-pectinate could entrap only 0.01 mg DNA when the weight ratio of pectin: MgCl 2 :DNA was 1:100:0.01 The transfection efficiency of both Ca-pectinate and Mg-pectinate nanoparticles yielded relatively low levels of green fluorescent protein expression and low cytotoxicity in Huh7 cells. Given the negligible cytotoxic effects, these pectinate micro/nanoparticles can be considered as potential candidates for use as safe gene delivery carriers.

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Section: Preparation Of Pectinate Microparticlesmentioning

confidence: 99%

Section: In Vitro Gene Expressionmentioning

confidence: 99%

Development and Characterization of Pectinate Micro/Nanoparticles for Gene Delivery

et al. 2008

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“…Bhakta et al stellten DNA-funktionalisierte Magnesiumphosphat-und Manganphosphat-Nanopartikel mit Durchmessern von 100-130 nm her. [180] Brash et al berichteten über die Herstellung und Charakterisierung von Strontiumphosphat-Nanopartikeln und ihre Anwendung sowohl für die transiente als auch für eine stabile Transfektion. [181] Im Hinblick auf die Biokompatibilität von Calciumphosphat-Nanopartikeln berichteten Liu et al über eine apoptotische Wirkung nichtfunktionalisierter Calciumphosphat-Nanopartikel mit einem Durchmesser von ca.…”

Section: Kohlenstoffnanoröhrenunclassified

Anorganische Nanopartikel zum Transport von Nucleinsäuren in Zellen

Sokolova

Epple

2008

Angewandte Chemie

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Die Aufnahme von Nucleinsäuren (DNA oder RNA) in lebende Zellen wird als Transfektion bezeichnet. Diese wichtige Technik der modernen Biochemie und Molekularbiologie ermöglicht durch die Einschleusung von genetischem Material sowohl die Hochregulierung der Produktion bestimmter Proteine als auch die gezielte Inhibition der Proteinsynthese (Gen‐Stummschaltung). Da Nucleinsäuren die Zellwand nicht allein durchdringen können, bedarf es geeigneter Träger. Neben viralen, polymeren und liposomalen Trägern sind anorganische Nanopartikel für diese Aufgabe besonders geeignet, da sie in vielfältiger Natur hergestellt und oberflächenfunktionalisiert werden können. In diesem Aufsatz wird der gegenwärtige Stand der Forschung aus chemischer Sicht vorgestellt; Vor‐ und Nachteile der verfügbaren Methoden werden diskutiert.

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“…Although there is a long list of nanoparticles under investigation, inorganic nanoparticles such as metals, metal oxides, ceramics etc., have received increased attention in the field of cancer nanotechnology because of their unique optical, magnetic, electric or mechanical properties in their nanostructures. Some key inorganic nanoparticles like calcium phosphate (10~100 nm), iron oxide (5~10 nm), silica (3~100 nm), zinc oxide (3~60 nm), zinc sulfide (3~50 nm), and magnesium and manganous phosphate has been extensively used for gene based drug delivery [5,6].…”

mentioning

confidence: 99%

Biodegradable and Bioabsorbable Inorganic Particles in Cancer Nanotechnology

Bhattarai

2013

J Nanomedic Nanotechnol

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DNA encapsulated magnesium and manganous phosphate nanoparticles: potential non-viral vectors for gene delivery

Cited by 66 publications

References 23 publications

Development and Characterization of Pectinate Micro/Nanoparticles for Gene Delivery

Development and Characterization of Pectinate Micro/Nanoparticles for Gene Delivery

Anorganische Nanopartikel zum Transport von Nucleinsäuren in Zellen

Biodegradable and Bioabsorbable Inorganic Particles in Cancer Nanotechnology

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