DNA droplets for intelligent and dynamical artificial cells: from the viewpoint of computation and non-equilibrium systems

Takinoue, Masahiro

doi:10.1098/rsfs.2023.0021

Cited by 7 publications

(5 citation statements)

References 90 publications

(182 reference statements)

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“…Genetic materials like DNA or RNA can be included into artificial cells accompanied with their protein contents to assist in genetically expressive and regulatory activities. These artificial cells have synthetic DNA sequences coding for particular genes or regulatory elements (28). Such sequences are caged inside the cell to facilitate gene expression, protein synthesis or cellular signaling.…”

Section: How Are Artifical Cell Designed?mentioning

confidence: 99%

“…Such sequences are caged inside the cell to facilitate gene expression, protein synthesis or cellular signaling. Researchers introduce genetic material in these cells; hence, artificial cells can be manipulated to acquire particular functions which may enable them respond to external factors or undertake predetermined actions (28).…”

Section: How Are Artifical Cell Designed?mentioning

confidence: 99%

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Artificial cells: A potentially groundbreaking field of research and therapy

Dundar,

Baki Yildirim,

Yildirim

et al. 2024

The EuroBiotech Journal

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Artificial cells are synthetic constructs that mimic the architecture and functions of biological cells. Artificial cells are designed to replicate the fundamental principles of biological systems while also have the ability to exhibit novel features and functionalities that have not been achieved before. Mainly, Artificial cells are made up of a basic structure like a cell membrane, nucleus, cytoplasm and cellular organelles. Nanotechnology has been used to make substances that possess accurate performance in these structures. There are many roles that artificial cells can play such as drug delivery, bio-sensors, medical applications and energy storage. An additional prominent facet of this technology is interaction with biological systems. The possibility of synthetic cells being compatible with living organisms opens up the potential for interfering with specific biological activities. This element is one of the key areas of research in medicine, aimed at developing novel therapies and comprehending life processes. Nevertheless, artificial cell technology is not exempt from ethical and safety concerns. The interplay between these structures and biological systems may give rise to questions regarding their controllability and safety. Hence, the pursuit of artificial cell research seeks to reconcile ethical and safety concerns with the potential advantages of this technology.

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Section: How Are Artifical Cell Designed?mentioning

confidence: 99%

Section: How Are Artifical Cell Designed?mentioning

confidence: 99%

Artificial cells: A potentially groundbreaking field of research and therapy

Dundar,

Baki Yildirim,

Yildirim

et al. 2024

The EuroBiotech Journal

View full text Add to dashboard Cite

show abstract

“…SCs with smaller internal compartments are used as models of eukaryotic cells, or just to achieve specific and more complex behaviors [ 131 ]. Interested readers should refer to recent reviews, e.g., [ 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 , 140 , 141 , 142 , 143 , 144 , 145 , 146 , 147 , 148 , 149 ].…”

Section: Appendix A1 Pioneering Work On Bottom-up Synthetic Cellsmentioning

confidence: 99%

Chemical Systems for Wetware Artificial Life: Selected Perspectives in Synthetic Cell Research

Stano

2023

IJMS

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The recent and important advances in bottom-up synthetic biology (SB), in particular in the field of the so-called “synthetic cells” (SCs) (or “artificial cells”, or “protocells”), lead us to consider the role of wetware technologies in the “Sciences of Artificial”, where they constitute the third pillar, alongside the more well-known pillars hardware (robotics) and software (Artificial Intelligence, AI). In this article, it will be highlighted how wetware approaches can help to model life and cognition from a unique perspective, complementary to robotics and AI. It is suggested that, through SB, it is possible to explore novel forms of bio-inspired technologies and systems, in particular chemical AI. Furthermore, attention is paid to the concept of semantic information and its quantification, following the strategy recently introduced by Kolchinsky and Wolpert. Semantic information, in turn, is linked to the processes of generation of “meaning”, interpreted here through the lens of autonomy and cognition in artificial systems, emphasizing its role in chemical ones.

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“…This feature is best used to construct designed-shaped nanostructures and molecular computing systems. In recent years, the programmability of DNA has been extended to the LLPS [ 5 , 6 ], attracting worldwide attention in artificial cell studies. The LLPS of DNA nanostructures was observed when branched DNA nanostructures, such as “Y-shape” and “X-shape,” were mixed in a solution.…”

Section: Introductionmentioning

confidence: 99%

Pioneering artificial cell-like structures with DNA nanotechnology-based liquid-liquid phase separation

Sato,

Takinoue

2024

BIOPHYSICS

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Recent studies have revealed that liquid-liquid phase separation (LLPS) plays crucial roles in various cellular functions. Droplets formed via LLPS within cells, often referred to as membraneless organelles, serve to concentrate specific molecules, thus enhancing biochemical reactions. Artificial LLPS systems have been utilized to construct synthetic cell models, employing a range of synthetic molecules. LLPS systems based on DNA nanotechnology are particularly notable for their designable characteristics in droplet formation, dynamics, properties, and functionalities. This review surveys recent advancements in DNA-based LLPS systems, underscoring the programmability afforded by DNA’s base-pair specific interactions. We discuss the fundamentals of DNA droplet formation, including temperature-dependence and physical properties, along with the precise control achievable through sequence design. Attention is given to the phase separation of DNA nanostructures on two-dimensional closed interfaces, which results in spatial pattern formation at the interface. Furthermore, we spotlight the potential of DNA droplet computing for cancer diagnostics through specific microRNA pattern recognition. We envision that DNA-based LLPS presents a versatile platform for the exploration of cellular mimicry and opens innovative ways for the development of functional synthetic cells.

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DNA droplets for intelligent and dynamical artificial cells: from the viewpoint of computation and non-equilibrium systems

Cited by 7 publications

References 90 publications

Artificial cells: A potentially groundbreaking field of research and therapy

Artificial cells: A potentially groundbreaking field of research and therapy

Chemical Systems for Wetware Artificial Life: Selected Perspectives in Synthetic Cell Research

Pioneering artificial cell-like structures with DNA nanotechnology-based liquid-liquid phase separation

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