DNA double‐strand breaks, recombination and synapsis: the timing of meiosis differs in grasshoppers and flies

Viera, Alberto; Santos, Juan Luis; Page, Jesús; Parra, María Teresa; Calvente, Adela; Cifuentes, Marta; Gómez, Rocío; Lira, Renée; Suja, José Á.; Rufas, Julio S.

doi:10.1038/sj.embor.7400112

Cited by 39 publications

(68 citation statements)

References 25 publications

(43 reference statements)

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“…Numerous S-and L-foci were detectable from the onset of zygotene, and the number of L-foci declined gradually until becoming a rare event throughout pachytene and diplotene. By contrast, we still found at pachytene multiple S-foci along the SC, as previously described in human and grasshopper (Lenzi et al, 2005;Roig et al, 2004;Viera et al, 2004). Their number progressively decreased from about 120 to 50 from early to late pachytene and finally to zero at diplotene.…”

Section: Discussionmentioning

confidence: 54%

“…The progressive disappearance of these foci coincides with the time course of repair of meiotic DSBs (Keogh et al, 2006) and a subset of them colocalize with RAD51/DMC1 and MLH1 foci at early and late pachytene, respectively. Such collocations were also observed in human and grasshopper spermatocytes (Roig et al, 2004;Viera et al, 2004). Finally, in contrast to Spo11…”

Section: Discussionmentioning

confidence: 92%

“…Thus, because ␥-H2AX foci on synaptonemal complexes (SCs) are markers of SPO11-dependent DSB repair at early stages of the meiotic prophase (Hunter et al, 2001;Mahadevaiah et al, 2001), they might still be detectable at pachytene. Accordingly, ␥-H2AX foci were detected along SCs in human and grasshopper pachytene cells, and colocalized with repair protein foci (Lenzi et al, 2005;Roig et al, 2004;Viera et al, 2004). By contrast, no ␥-H2AX foci were observed at this stage in mouse spermatocytes by Mahadevaiah et al (Mahadevaiah et al, 2001), suggesting differences between species in the dynamics of H2AX phosphorylation/dephosphorylation (Lenzi et al, 2005).…”

Section: Introductionmentioning

confidence: 80%

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Characterization ofSpo11-dependent and independent phospho-H2AX foci during meiotic prophase I in the male mouse

Chicheportiche

Bernardino-Sgherri

Massy

et al. 2007

Journal of Cell Science

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-/-spermatocytes. By contrast, the L-foci number was very low through pachytene. Based on the analysis of ␥-H2AX labeling after irradiation of spermatocytes, the formation of DSBs clearly induced L-foci formation. Upon DSB repair, these foci appear to be processed and lead to the above mentioned S-foci. The presence of L-foci in wild-type pachytene and diplotene could therefore reflect delayed or unregulated DSB repair events. Interestingly, their distribution was different in Spo11 +/-spermatocytes compared with Spo11 +/+ spermatocytes, where DSB repair might be differently regulated as a response to homeostatic control of crossing-over. The presence of these L-foci in Spo11 -/-spermatocytes raises the interesting possibility of yet uncharacterized alterations in DNA or chromosome structure in Spo11 -/-cells.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 80%

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Characterization ofSpo11-dependent and independent phospho-H2AX foci during meiotic prophase I in the male mouse

Chicheportiche

Bernardino-Sgherri

Massy

et al. 2007

Journal of Cell Science

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“…recombination and synapsis in meiosis differ in flies and grasshoppers, indicating the existence of divergence in the regulatory mechanisms of chromosomal behavior in meiosis even in two species phylogenetically close to each other [18]. Molecular components of the SC reported to date are surprisingly diverged, at least at the amino acid level, suggesting the existence of species-specificity in precise roles of each component of the SC.…”

mentioning

confidence: 99%

Structural components of the synaptonemal complex, SYCP1 and SYCP3, in the medaka fish Oryzias latipes

Iwai

Yoshii

Yokota

et al. 2006

Experimental Cell Research

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1 SummaryThe synaptonemal complex (SC) is a meiosis-specific structure essential for synapsis of homologous chromosomes. For the first time in any non-mammalian vertebrates, we have isolated cDNA clones encoding two structural components of the SC, SYCP1 and SYCP3, in the medaka, and investigated their protein expression during gametogenesis.As in the case of mammals, medaka SYCP1 and SYCP3 are expressed solely in meiotically dividing cells. In the diplotene stage, SYCP1 is diminished at desynaptic regions of chromosomes and completely lost on the chromosomes at later stages.SYCP3 is localized along the arm and centromeric regions of chromosomes at metaphase I, and its existence on the whole chromosomes persists up to anaphase I, a situation different from that reported in the mouse, in which SYCP3 is confined to the centromeric regions but lost on the arm regions at metaphase I. Thus, the expression patterns of SC components are different in mammals and fish despite the resemblance in morphological structure of the SC, suggesting divergence in the function of the SC in regulation of meiosis-specific chromosomal behavior. Since the antibody against medaka SYCP3 is cross-reactive to other fishes, it should be generally useful for a meiosis-specific marker in fish germ cells.

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“…Testes from adult E. plorans and L. migratoria males were removed and placed in 1 ml of 0.5% Triton X-100 in PBS for 5 min and then washed with PBS, as previously described [48].…”

Section: Methodsmentioning

confidence: 99%

Sequential Loading of Cohesin Subunits during the First Meiotic Prophase of Grasshoppers

Valdeolmillos¹,

Viera²,

Page³

et al. 2005

PLoS Genet

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The cohesin complexes play a key role in chromosome segregation during both mitosis and meiosis. They establish sister chromatid cohesion between duplicating DNA molecules during S-phase, but they also have an important role during postreplicative double-strand break repair in mitosis, as well as during recombination between homologous chromosomes in meiosis. An additional function in meiosis is related to the sister kinetochore cohesion, so they can be pulled by microtubules to the same pole at anaphase I. Data about the dynamics of cohesin subunits during meiosis are scarce; therefore, it is of great interest to characterize how the formation of the cohesin complexes is achieved in order to understand the roles of the different subunits within them. We have investigated the spatio-temporal distribution of three different cohesin subunits in prophase I grasshopper spermatocytes. We found that structural maintenance of chromosome protein 3 (SMC3) appears as early as preleptotene, and its localization resembles the location of the unsynapsed axial elements, whereas radiation-sensitive mutant 21 (RAD21) (sister chromatid cohesion protein 1, SCC1) and stromal antigen protein 1 (SA1) (sister chromatid cohesion protein 3, SCC3) are not visualized until zygotene, since they are located in the synapsed regions of the bivalents. During pachytene, the distribution of the three cohesin subunits is very similar and all appear along the trajectories of the lateral elements of the autosomal synaptonemal complexes. However, whereas SMC3 also appears over the single and unsynapsed X chromosome, RAD21 and SA1 do not. We conclude that the loading of SMC3 and the non-SMC subunits, RAD21 and SA1, occurs in different steps throughout prophase I grasshopper meiosis. These results strongly suggest the participation of SMC3 in the initial cohesin axis formation as early as preleptotene, thus contributing to sister chromatid cohesion, with a later association of both RAD21 and SA1 subunits at zygotene to reinforce and stabilize the bivalent structure. Therefore, we speculate that more than one cohesin complex participates in the sister chromatid cohesion at prophase I.Citation: Valdeolmillos AM, Viera A, Page J, Prieto I, Santos JL, et al. (2007) Sequential loading of cohesin subunits during the first meiotic prophase of grasshoppers. PLoS Genet 3(2): e28.

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DNA double‐strand breaks, recombination and synapsis: the timing of meiosis differs in grasshoppers and flies

Cited by 39 publications

References 25 publications

Characterization ofSpo11-dependent and independent phospho-H2AX foci during meiotic prophase I in the male mouse

Characterization ofSpo11-dependent and independent phospho-H2AX foci during meiotic prophase I in the male mouse

Structural components of the synaptonemal complex, SYCP1 and SYCP3, in the medaka fish Oryzias latipes

Sequential Loading of Cohesin Subunits during the First Meiotic Prophase of Grasshoppers

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