DNA divergence as a criterion of a sweet pepper (Capsicum annuum L.) selection for heterosis

Шаптуренко, М. Н.; Тарутина, Л. А.; Mishin, L.A.; Kilchevsky, A. V.; Хотылева, Л. В.

doi:10.1134/s1022795414020148

Cited by 10 publications

(11 citation statements)

References 21 publications

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“…However, few studies focus on the genetic dissimilarity with ISSR markers to estimate relevant biometric parameters for plant breeding. Shapturenko et al (2014) used nine ISSR primers for 23 chili accessions and achieved 28% polymorphism in 56 amplified bands. Based on genetic distances, the 10 most divergent parents were selected and intercrossed with each other and the hybrids evaluated for heterosis and mean fruit yield.…”

Section: Morphological and Molecular Markersmentioning

confidence: 99%

Prediction of industrial tomato hybrids from agronomic traits and ISSR molecular markers

Figueiredo¹,

Resende²,

Faria³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Heterosis is a highly relevant phenomenon in plant breeding. This condition is usually established in hybrids derived from crosses of highly divergent parents. The success of a breeder in obtaining heterosis is directly related to the correct identification of genetically contrasting parents. Currently, the diallel cross is the most commonly used methodology to detect contrasting parents; however, it is a time-and cost-consuming procedure. Therefore, new tools capable of performing this task quickly and accurately are required. Thus, the purpose of this study was to estimate the genetic divergence in industrial tomato lines, based on agronomic traits, and to compare with estimates obtained using inter-simple sequence repeat (ISSR) molecular markers. The genetic divergence among 10 industrial tomato lines, based on nine morphological characters and 12 ISSR primers was analyzed. For data analysis, Pearson and Spearman correlation coefficients were calculated between the genetic dissimilarity measures estimated by Mahalanobis distance and Jaccard's coefficient of genetic dissimilarity from the heterosis estimates, combining ability, and means of important traits of industrial tomato. The ISSR markers efficiently detected contrasting parents for hybrid production in tomato. Parent RVTD-08 was indicated as the most divergent, both by molecular and morphological markers, that positively contributed to increased heterosis and by the specific combining ability in the crosses in which it participated. The genetic dissimilarity estimated by ISSR molecular markers aided the identification of the best hybrids of the experiment in terms of total fruit yield, pulp yield, and soluble solids content.

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Section: Morphological and Molecular Markersmentioning

confidence: 99%

Prediction of industrial tomato hybrids from agronomic traits and ISSR molecular markers

Figueiredo¹,

Resende²,

Faria³

et al. 2016

Genet. Mol. Res.

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“…Tilahun et al (2013) used 20 primers to analyze 30 genotypes of C. annuum L. and obtained 78 products, 83% of which were polymorphic. Much lower polymorphism rates were observed in the studies of Shapturenko et al (2014), where the use of 21 primers to analyze 10 C. annuum L. lines revealed a polymorphism Table 2. Coefficients of genetic distance of Capsicum annuum L.…”

Section: Resultsmentioning

confidence: 87%

The application of the RAPD technique in the identification of the genetic diversity of cultivated forms of Capsicum annuum L.

Niklas

Olszewska

2021

bta

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Background. The extensive use of pepper fruit creates a constant demand for new cultivars with specific agromorphological properties. The wide variety of breeding materials of this species means that methods based on morphological traits descriptions are not always sufficient to allow for their identification. Genetic homogeneity must be guaranteed to ensure repeatability of phenotypic traits. Most often, molecular analyses characterizing diversity at the DNA level are used for this purpose. Material and methods. The PCR-RAPD technique was used for molecular analysis of the generative offspring of three cultivars of pepper: "Anchi", "Luba" and "Sono" and their forms of different fruit colour. The genetic distance between the tested genotypes was determined using the Nei and Li formula. Results. The reaction with 26 RAPD primers resulted in a total of 262 products and 5.2% of them were polymorphic bands. Eight of the used primers generated 12 polymorphic products that differentiated the tested genotypes. The "Anchi" cultivar was identified by the primers A07, K10, Q07 and AE10. Starter Q07 identified as well the "Luba" cultivar. Reactions carried out with primers B10 and RAD1 identified the "Sono" cultivar. In addition, primer A15 generated products that made it possible to distinguish yellow-fruit and red-fruit forms within the "Luba" and "Sono" cultivars. Conclusion. The analyses showed a low degree of genetic distance between C. annuum L. cultivars confirming the genetic homogeneity of the examined groups of plants and creating and opportunity for molecular identification of the genetic diversity within the "Luba" and "Sono" cultivars.

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“…Tilahun et al [23], assessing 30 genotypes of C. annuum L., obtained 83% of the polymorphic products. Polymorphism at the level of 26% was observed in the study of Shapturenko et al [24] in the analysis of 10 C. annuum L. lines. In the presented experiment, in which the progeny of two androgenic regenerants obtained in hybrid anther cultures (C. annuum L. ATZ × C. annuum L. 'Corno di toro')F 2 were studied, 10 RAPD primers were used.…”

Section: Discussionmentioning

confidence: 84%

Androgenesis—Technology for Obtaining Genetically Stable Breeding Material of Capsicum annuum L.

Olszewska

Tomaszewska-Sowa

2021

Agriculture

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Androgenesis in vitro is a basic method of obtaining haploid plants and DH (doubled haploid) lines of major crops such as potato, rapeseed, tomato, pepper, wheat, maize, and barley, and also many different minor crops and species with lower agricultural impact. Diploid plants appearing among androgenic regenerants are the effect of spontaneous doubling of the chromosome number in haploid cells during an embryo’s early developmental stages and are valuable fully homozygous breeding material. The subject of the presented research is spontaneous diploidization occurring in the development of androgenic, haploid pepper regenerants. In the presented experiment, the formation of diploid seeds was observed in the progeny of an androgenic, haploid plant derived in an anther culture of a hybrid (Capsicum annuum L. ATZ × Capsicum annuum L. `Corno di toro`)F2. Agromorphological and molecular analyses concerned eight diploid plants being progeny of the anther-derived haploid regenerant. Five of the plants constituted a phenotypically balanced group with valuable agromorphological features. Their genetic homogeneity was confirmed using 10 RAPD markers and 16 ISSR markers. Based on the results, it was concluded that anther-derived haploid plants of Capsicum can be the source of diploid, apomictic seeds, and the obtained offspring may constitute genetically stable, valuable breeding material.

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DNA divergence as a criterion of a sweet pepper (Capsicum annuum L.) selection for heterosis

Cited by 10 publications

References 21 publications

Prediction of industrial tomato hybrids from agronomic traits and ISSR molecular markers

Prediction of industrial tomato hybrids from agronomic traits and ISSR molecular markers

The application of the RAPD technique in the identification of the genetic diversity of cultivated forms of Capsicum annuum L.

Androgenesis—Technology for Obtaining Genetically Stable Breeding Material of Capsicum annuum L.

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