DNA diagnostic for <i>Armillaria</i> species in British Columbia: within and between species variation in the IGS-1 and IGS-2 regions

White, Eleanor; Dubetz, C. P.; Cruickshank, M. G.; Morrison, D. J.

doi:10.1080/00275514.1998.12026888

Cited by 42 publications

(20 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Trees of each age and species with natural infections of A. ostoyae on their roots were identified by careful excavation. Lesions were identified by the presence of resinosis, rhizomorphs and mycelial fans in the bark, and A. ostoyae was confirmed by isolation of pure cultures and molecular identification using the methods outlined in White et al. (1998).…”

Section: 1 Site and Tree Selectionmentioning

confidence: 99%

Necrophylactic periderm formation in the roots of western larch and Douglas‐fir trees infected with Armillaria ostoyae. II. The response to the pathogen

2004

Self Cite

View full text Add to dashboard Cite

Summary Periderm formation was studied in bark samples collected from the roots of western larch (Larix occidentalis) and Douglas‐fir (Pseudotsuga menziesii) trees infected with Armillaria ostoyae. Necrophylactic periderms were formed in advance of infection and successfully restricted continued fungal spread in 68 and 45% of the samples collected from 10‐ and 27‐year‐old western larch, respectively. However, all periderms formed in 11‐ and 25‐year‐old Douglas‐fir had been breached by the advancing fungus. In both species, necrophylactic periderms were commonly breached at the junction of the periderm with the vascular cambium. In western larch, stone phellem often comprised the external phellem layer of necrophylactic periderms with multiple bands of phellem. In 27‐year‐old western larch, infection was often confined to discrete lesions bounded by multiple periderms with multiple bands of phellem. In both tree species, phellem production was greater in response to infection than in response to abiotic wounding.

show abstract

Section: 1 Site and Tree Selectionmentioning

confidence: 99%

Necrophylactic periderm formation in the roots of western larch and Douglas‐fir trees infected with Armillaria ostoyae. II. The response to the pathogen

2004

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several molecular methods were developed to augment identification of Armillaria species and determine phylogenetic relationships among Armillaria species (Anderson et al 1987Jahnke et al 1987;Smith and Anderson 1989;Smith et al 1990;Anderson and Stasovski 1992;Miller et al 1994;Harrington and Wingfield 1995;Schulze et al 1995Schulze et al , 1997Chillali et al 1998;Piercey-Normore et al 1998;Terashima et al 1998;White et al 1998;Coetzee et al 2000Coetzee et al , 2001Coetzee et al , 2003Kim et al 2000;Otieno et al 2003;Gezahgne et al 2004;Pérez-Sierra et al 2004). Anderson and Stasovski (1992) determined the DNA sequences of intergenic spacer (IGS-1) and assessed phylogenetic relationships among the NABS of Armillaria.…”

Section: Introductionmentioning

confidence: 99%

Characterization of North American Armillaria species: genetic relationships determined by ribosomal DNA sequences and AFLP markers

Kim¹,

Klopfenstein²,

Hanna³

et al. 2006

Forest Pathology

View full text Add to dashboard Cite

Phylogenetic and genetic relationships among 10 North American Armillaria species were analysed using sequence data from ribosomal DNA (rDNA), including intergenic spacer (IGS-1), internal transcribed spacers with associated 5.8S (ITS + 5.8S), and nuclear large subunit rDNA (nLSU), and amplified fragment length polymorphism (AFLP) markers. Based on rDNA sequence data, the nLSU region is less variable among Armillaria species than the ITS + 5.8S and IGS-1 regions (nLSU < ITS + 5.8S < IGS-1). Phylogenetic analyses of the rDNA sequences suggested Armillaria mellea, A. tabescens and A. nabsnona are well separated from the remaining Armillaria species (A. ostoyae, A. gemina, A. calvescens, A. sinapina, A. gallica, NABS X and A. cepistipes). Several Armillaria species (A. calvescens, A. sinapina, A. gallica, NABS X and A. cepistipes) clustered together based on rDNA sequencing data. Based on the isolates used in this study, it appears that techniques based on IGS-1, ITS + 5.8S, and/or D-domain/3¢ ends of nLSU are not reliable for distinguishing A. calvescens, A. sinapina, A. gallica and A. cepistipes. However, AFLP data provided delineation among these species, and AFLP analysis supported taxonomic classification established by conventional methods (morphology and interfertility tests). Our results indicate that AFLP genetic markers offer potential for distinguishing currently recognized North American Biological Species (NABS) of Armillaria in future biological, ecological and taxonomic studies.

show abstract

“…Armillaria ostoyae is the cause of Armillaria root disease of conifers in western North America (Wargo and Shaw 1985), and isolations from diseased trees indicate that it is the only species causing primary disease on conifers in British Columbia (Morrison et al 1985;White et al 1998). In British Columbia, A. ostoyae occurs south of about 53°N (Morrison et al 1985) on most species of conifers (Allen et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Visible versus actual incidence of Armillaria root disease in juvenile coniferous stands in the southern interior of British Columbia

Morrison¹,

Pellow²,

Norris³

et al. 2000

Can. J. For. Res.

View full text Add to dashboard Cite

The relationship between aboveground symptoms and belowground incidence of Armillaria ostoyae (Romagn.) Herink on conifers in 13-to 24-year-old stands was investigated at five sites in each of the dry, moist, and wet climatic regions in the Nelson forest region, British Columbia. All trees >1.3 m in height in 0.01-ha circular plots centred on a tree killed fewer than two or more than five years previously or located where there were no symptomatic trees were removed from the soil by an excavator. The location and host response at each A. ostoyae lesion on root systems were recorded. Significant differences in belowground incidence were seen among climatic regions and plot types, with distance from the centre of plots, and between planted and naturally regenerated trees. Belowground incidence was related to the percentage of putatively colonized stumps within and adjacent to plots. There were significant differences among climatic regions in the intensity of infection, host reaction to infection, and percentage of diseased trees showing aboveground symptoms. These results have implications for interpreting results of surveys for Armillaria root disease in juvenile stands and for tending of such stands.Résumé : Cette étude porte sur la relation entre les symptômes présents sur les parties aériennes et l'incidence d'Armillaria ostoyae (Romagn.) Herink sur les racines de conifères dans des peuplements âgés de 13 à 24 ans. L'étude a été réalisée dans cinq sites dans chacune des zones climatiques sèche, semi-humide et humide, de la région forestière de Nelson, en Colombie-Britannique. Tous les arbres plus hauts que 1,3 m dans des parcelles circulaires de 0,01 ha ayant comme centre un arbre mort il y a moins de deux ans ou plus de cinq ans, ou se trouvant dans un lieu exempt d'arbres portant des symptômes, ont été extraits du sol avec une excavatrice. La position des lésions dues à l'A. ostoyae sur le système racinaire et la réaction de l'hôte ont été notées. L'incidence du champignon sur les racines différait significativement selon la zone climatique, le type de parcelles, la distance à partir du centre de la parcelle et selon que les arbres avaient été plantés ou provenaient de la régénération naturelle. L'incidence du champignon sur les racines était reliée au pourcentage de souches apparemment colonisées à l'intérieur ou à proximité des parcelles. La sévérité de l'infection, la réaction de l'hôte et le pourcentage d'arbres malades montrant des symptômes sur les parties aériennes étaient significativement différents selon la zone climatique. Ces résultats peuvent avoir un impact sur l'interprétation des résultats des relevés du pourridié-agaric dans les jeunes peuplements et sur la façon de traiter de tels peuplements. Morrison et al. 414

show abstract

DNA diagnostic for Armillaria species in British Columbia: within and between species variation in the IGS-1 and IGS-2 regions

Cited by 42 publications

References 20 publications

Necrophylactic periderm formation in the roots of western larch and Douglas‐fir trees infected with Armillaria ostoyae. II. The response to the pathogen

Necrophylactic periderm formation in the roots of western larch and Douglas‐fir trees infected with Armillaria ostoyae. II. The response to the pathogen

Characterization of North American Armillaria species: genetic relationships determined by ribosomal DNA sequences and AFLP markers

Visible versus actual incidence of Armillaria root disease in juvenile coniferous stands in the southern interior of British Columbia

Contact Info

Product

Resources

About