DNA Diagnostic for Armillaria Species in British Columbia: Within and between Species Variation in the IGS-1 and IGS-2 Regions

White, E. E.; Dubetz, C. P.; Cruickshank, M. G.; Morrison, D. J.

doi:10.2307/3761021

Cited by 23 publications

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“…focussing on the ITS, IGS1, and LSU regions of rDNA, Hanna et al (2007) reported that heterogeneous rDNA products were common in all of these regions. rDNA heterozygosity has also been reported in diploid European A. gallica isolates (Pérez-Sierra et al 1999), A. sinapina (White et al 1998), and Armillaria nabsnona . Kim et al (2000), in their investigation of the nuclear DNA contents of North American Armillaria spp., found evidence of triploidy in one A. ostoyae isolate.…”

Section: S Gene and Igs2 Region Sequencesmentioning

confidence: 92%

“…Duchesne and Anderson (1990) confirmed the location and direction of transcription of the 5S gene in Armillaria. White et al (1998) distinguished British Columbia isolates of Armillaria gallica from Armillaria sinapina by AluI RFLP digest of IGS2 PCR products but did not take this work further, nor did they publish any DNA sequence information for the IGS2 region. The objectives of this study were as follows: (i) obtain complete DNA sequences for the 5S gene and the IGS2 region of the four most commonly isolated Armillaria spp.…”

Section: Introductionmentioning

confidence: 99%

“…Phylogenetic and RFLP analyses of the ITS and IGS1 regions of Armillaria are useful tools, especially in initially characterizing species groups in a particular region or country, but they fall short as a practical means of confidently distinguishing Armillaria spp. because of the high sequence similarity between species (Harrington and Wingfield 1995;Banik et al1996;White et al 1998).…”

Section: Introductionmentioning

confidence: 99%

“…Cycle modified from White et al (1998). dition to the nine isolates that were sequenced over the full IGS2 region (Table S2 2 ).…”

mentioning

confidence: 99%

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Identification protocol for sixArmillariaspecies from northeastern North America

McLaughlin

Hsiang

2010

Can. J. For. Res.

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DNA sequences (~3 kb long) extending from the intergenic spacer 1 (IGS1) region to the 18S gene were obtained for isolates of Armillaria ostoyae, Armillaria calvescens, Armillaria gallica, and Armillaria sinapina. Additional investigation of 16 A. ostoyae, 11 Armillaria gemina, 21 A. calvescens, 18 A. gallica, and 15 A. sinapina isolates produced 117 sequences spanning the 3' end of the IGS1 through the 5S gene and into the 5' end of the IGS2 region. Additional sequences spanning the 3' IGS2 to 5' 18S gene region were obtained for two A. ostoyae, three A. gemina, two A. calvescens, two A. gallica, and three A. sinapina isolates. This is the first report of complete IGS2 sequences from Armillaria spp. A species identification protocol involving species-specific primers and restriction fragment length polymorphism analysis was devised based on species-specific polymorphisms. The protocol successfully identified all 16 A. ostoyae, 11 A. gemina, three of three Armillaria mellea, 18 A. gallica, 14 of 15 A. sinapina (11/12 diploid and 3/3 haploid), and 14 of 21 A. calvescens (13/15 diploid and 1/6 haploid) isolates included in this study. To the best of our knowledge, this success rate has not been matched by other methods.

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Section: S Gene and Igs2 Region Sequencesmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Cycle modified from White et al (1998). dition to the nine isolates that were sequenced over the full IGS2 region (Table S2 2 ).…”

mentioning

confidence: 99%

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Identification protocol for sixArmillariaspecies from northeastern North America

McLaughlin

Hsiang

2010

Can. J. For. Res.

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“…Their PCR-based method is especially practical because mycelial scrapings frequently provide sufficient template DNA for species identification. Subsequently, this method has been widely applied to augment identification of Armillaria species (Banik and Burdsall 1998, Banik et a1 1996, Baumgartner et a1 1997, Frontz et a1 1998, Sung et a1 1997, Volk et a1 1996, White et al 1998.…”

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confidence: 99%

Characterization of North American Armillaria Species by Nuclear DNA Content and RFLP Analysis

et al. 2000

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Advances toward DNA-based identification and phylogeny of North American Armillaria species using elongation factor-1 alpha gene

et al. 2012

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The translation elongation factor-1 alpha (EF-1a) gene was used to examine the phylogenetic relationships among 30 previously characterized isolates representing ten North American Armillaria species:American biological species X, A. cepistipes, and A. tabescens. The phylogenetic relationships revealed clear separation of all ten North American Armillaria species, with the exception of one A. gallica isolate that perhaps represents an unnamed cryptic species. These results indicate that the EF-1a gene could potentially serve as a diagnostic tool for distinguishing among currently recognized North American biological species of Armillaria.

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DNA Diagnostic for Armillaria Species in British Columbia: Within and between Species Variation in the IGS-1 and IGS-2 Regions

Cited by 23 publications

References 0 publications

Identification protocol for sixArmillariaspecies from northeastern North America

Identification protocol for sixArmillariaspecies from northeastern North America

Characterization of North American Armillaria Species by Nuclear DNA Content and RFLP Analysis

Advances toward DNA-based identification and phylogeny of North American Armillaria species using elongation factor-1 alpha gene

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