DNA detection using commercial mobile phones

Lee, Dasheng; Chou, Wen Pin; Yeh, Shiou Hwei; Chen, Pei‐Jer; Chen, Ping‐Hei

doi:10.1016/j.bios.2011.04.036

Cited by 50 publications

(25 citation statements)

References 17 publications

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“…4b). Built-in cameras in mobile phones have been used as imaging platforms6263 as well as for the detection of disease biomarkers and infectious pathogens646566676869, and in this work a Smartphone camera was used to take an image of the strip followed by an application based on Image J software termed IJ_mobile to calculate the intensity of the bands, and these values were plotted using GraphPad Prism software in order to obtain the LOD of the assay. The data was normalized by subtracting the value obtained in the blank, and the LOD achieved was 1 × 10 −11 M, with a complete assay, combining amplification and detection at 37 °C, taking 15 minutes to complete.…”

Section: Resultsmentioning

confidence: 99%

Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay

Jauset‐Rubio

Svobodová

Mairal

et al. 2016

Sci Rep

141

113

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Sensitive, specific, rapid, inexpensive and easy-to-use nucleic acid tests for use at the point-of-need are critical for the emerging field of personalised medicine for which companion diagnostics are essential, as well as for application in low resource settings. Here we report on the development of a point-of-care nucleic acid lateral flow test for the direct detection of isothermally amplified DNA. The recombinase polymerase amplification method is modified slightly to use tailed primers, resulting in an amplicon with a duplex flanked by two single stranded DNA tails. This tailed amplicon facilitates detection via hybridisation to a surface immobilised oligonucleotide capture probe and a gold nanoparticle labelled reporter probe. A detection limit of 1 × 10−11 M (190 amol), equivalent to 8.67 × 105 copies of DNA was achieved, with the entire assay, both amplification and detection, being completed in less than 15 minutes at a constant temperature of 37 °C. The use of the tailed primers obviates the need for hapten labelling and consequent use of capture and reporter antibodies, whilst also avoiding the need for any post-amplification processing for the generation of single stranded DNA, thus presenting an assay that can facilely find application at the point of need.

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Section: Resultsmentioning

confidence: 99%

Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay

Jauset‐Rubio

Svobodová

Mairal

et al. 2016

Sci Rep

141

113

View full text Add to dashboard Cite

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“…Many detection technologies based on mobile phones have been developed for biodetection 10-23 . However, the sensitivity of the mobile phone camera is a limiting factor: it is currently too low to be effective for many of the diagnostic applications of these devices.…”

Section: Discussionmentioning

confidence: 99%

“…Several detection technologies based on mobile phones have been developed for biodetection, including an integrated rapid-diagnostic-test reader platform for lateral flow immunochromatographic assays 10 , capillary array based immunodetection for Escherichia coli 11 , wide-field fluorescent microscopy 12 , fluorescent imaging cytometry 13 , lensfree microscopy 14 , detection system s for melanoma or skin lesion 15-17 , loop-mediated isothermal amplification (LAMP) genetic testing device 18 , microchip ELISA-based detection of ovarian cancer HE4 biomarker in urine 19 , surface acoustic wave enhanced immunoassay 20 , a pocket-sized colorimetric reader 21 , phone-assisted microarray decoding platform for signal-enhanced mutation detection 22 , and mobile phone cameras for DNA detection 23 . However, all these technologies rely on the inherent sensitivity of the CMOS camera native to the mobile phone, which is less sensitive than detectors used for biodetection.…”

Section: Introductionmentioning

confidence: 99%

Capillary array waveguide amplified fluorescence detector for mHealth

Balsam

Bruck

Rasooly

2013

Sensors and Actuators B: Chemical

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Mobile Health (mHealth) analytical technologies are potentially useful for carrying out modern medical diagnostics in resource-poor settings. Effective mHealth devices for underserved populations need to be simple, low cost, and portable. Although cell phone cameras have been used for biodetection, their sensitivity is a limiting factor because currently it is too low to be effective for many mHealth applications, which depend on detection of weak fluorescent signals. To improve the sensitivity of portable phones, a capillary tube array was developed to amplify fluorescence signals using their waveguide properties. An array configured with 36 capillary tubes was demonstrated to have a ~100X increase in sensitivity, lowering the limit of detection (LOD) of mobile phones from 1000 nM to 10 nM for fluorescein. To confirm that the amplification was due to waveguide behavior, we coated the external surfaces of the capillaries with silver. The silver coating interfered with the waveguide behavior and diminished the fluorescence signal, thereby proving that the waveguide behavior was the main mechanism for enhancing optical sensitivity. The optical configuration described here is novel in several ways. First, the use of capillaries waveguide properties to improve detection of weak florescence signal is new. Second we describe here a three dimensional illumination system, while conventional angular laser waveguide illumination is spot (or line), which is functionally one-dimensional illumination, can illuminate only a single capillary or a single column (when a line generator is used) of capillaries and thus inherently limits the multiplexing capability of detection. The planar illumination demonstrated in this work enables illumination of a two dimensional capillary array (e.g. x columns and y rows of capillaries). In addition, the waveguide light propagation via the capillary wall provides a third dimension for illumination along the axis of the capillaries. Such an array can potentially be used for sensitive analysis of multiple fluorescent detection assays simultaneously. The simple phone based capillary array approach presented in this paper is capable of amplifying weak fluorescent signals thereby improving the sensitivity of optical detectors based on mobile phones. This may allow sensitive biological assays to be measured with low sensitivity detectors and may make mHealth practical for many diagnostics applications, especially in resource-poor and global health settings.

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“…As mentioned above, cellphones-based devices can sense biomarkers, provide counting capabilities, and imaging of cells / microorganisms for environmental monitoring and food safety, but may also be used for a variety of clinical applications [79,81,94,104]. The compatibility of cellphone platforms and various RDT formats clearly demonstrates successful quantitative and real-time analysis of immunochromatographic RDTs [89].…”

Section: Cellphone-based Diagnostics Toolsmentioning

confidence: 99%