DNA-Dependent RNA Polymerases from Artemia salina

Bagshaw, Joseph C.; Bernstein, Richard; Bond, Bernice H.

doi:10.1111/j.1432-0436.1978.tb00940.x

Cited by 9 publications

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2A). It can then be calculated that the average individual nucleus should contain about 6.2 pg, a value which agrees exactly with that reported based on chemical analysis of counted, isolated nuclei obtained from 24 h stage nauplii (42). This correspondence indicates that our conditions of homogenization are breaking virtually all cysts at every stage, for we are able to extract all DNA expected.…”

Section: O_nt Of R_na Sythsi Iuder Deelegmend Regusupporting

confidence: 88%

In vivotranscription from multiple spacer rRNA gene promoters during early development and evolution of the intergenic spacer in the brine shrimpArtemia

et al. 1987

View full text Add to dashboard Cite

The control of ribosomal RNA (rRNA) gene expression during development can be productively studied by examination of the relationship between promoter structure and function as well as the processing of primary transcripts. Toward this end total cell RNA was extracted from embryos at various stages and probed with cloned rRNA genes using the "dot blot" method. This exercise showed that rRNA gene expression is a stage-specific process and is thus under developmental control. S1 nuclease protection experiments localized fourteen different upstream DNA sites encoding 5'-termini of pre-rRNAs during this synthetic phase of development. There is no indication of any spacer fail-safe terminator function. The S1 approach contributed to the sequencing of several of the sites. Comparative sequence alignments reveal short conserved regions in DNAs corresponding to these sites, which are shown to fall into two structural classes. Sites 3, 4, 6 and 9 are proposed to function in transcription initiation and are found to have the consensus sequence 5'...T-A-T-A-T-Pu-Pu-Pu-G-Pu-Pu-G-T-C-A 3'. Sites 1, 2, 5 and 8 which are proposed to function in 5'-processing have the consensus sequence; 5'...Pu-G-T-Pu-T-T-G 3'. These short sequence conserved regions are hypothesized to serve as recognition signals for proteins within the rDNA transcription initiation complex and for 5'-processing enzymes, respectively. Sequencing of the intergenic spacer region from which a model for spacer evolution is derived shows that tandem ca 600 bp subrepeats explain much of the multiplicity observed within control sites.

show abstract

Section: O_nt Of R_na Sythsi Iuder Deelegmend Regusupporting

confidence: 88%

In vivotranscription from multiple spacer rRNA gene promoters during early development and evolution of the intergenic spacer in the brine shrimpArtemia

et al. 1987

View full text Add to dashboard Cite

show abstract

Epigenetics meets mathematics: Towards a quantitative understanding of chromatin biology

2012

View full text Add to dashboard Cite

How fast? How strong? How many? So what? Why do numbers matter in biology? Chromatin binding proteins are forever in motion, exchanging rapidly between bound and free pools. How do regulatory systems whose components are in constant flux ensure stability and flexibility? This review explores the application of quantitative and mathematical approaches to mechanisms of epigenetic regulation. We discuss methods for measuring kinetic parameters and protein quantities in living cells, and explore the insights that have been gained by quantifying and modelling dynamics of chromatin binding proteins.

show abstract

Gene Action Changes during Nonvertebrate Differentiation

Dillon¹

1983

The Inconstant Gene

View full text Add to dashboard Cite

DNA-Dependent RNA Polymerases from Artemia salina

Cited by 9 publications

References 24 publications

In vivotranscription from multiple spacer rRNA gene promoters during early development and evolution of the intergenic spacer in the brine shrimpArtemia

In vivotranscription from multiple spacer rRNA gene promoters during early development and evolution of the intergenic spacer in the brine shrimpArtemia

Epigenetics meets mathematics: Towards a quantitative understanding of chromatin biology

Gene Action Changes during Nonvertebrate Differentiation

Contact Info

Product

Resources

About