DNA‐Dependent Prussian Blue Nanoflowers for Biosensing, Catalysis and Imaging

Abstract

While shapes and surface properties of nanomaterials are known to play important roles in defining their properties, it remains challenging to finetune the morphologies systematically and predictably. Considering the extraordinary performance, prussian blue nanoparticles (PBNPs) are selected as the proof-of-concept nanomaterials. Herein, a DNA-dependence approach to fine-control the morphology of PBNPs via electrostatic interaction-mediated self-assembly of inorganic ions and protonated DNA is developed. The regulation of different DNA on the morphology of PBNPs is systematically investigated. 30-mer Oligo-C or -T (C30/T30) mediates formation of flowerlike PBNPs (PB nanoflowers), whereas cubic structure with different sizes is observed in the presence of 10-mer oligo-G or 30-mer Oligo-A (G10/A30). Detailed mechanism studies indicate that the protonation of nucleobases is the key factor for the morphological evolution. C30-dependent PB nanoflowers are superior to PB nanocubes in photothermal properties, peroxidase mimetic activity, photo-Fenton catalytic performance, and light scattering property, which present 1.2-, 3.78-, 1.58-, 1.93-fold improvement, respectively. Furthermore, PB nanoflowers mediated by the diblock DNA (sDNA; comprising C30 and complementary strands of the target DNA) unexpectedly acquire biorecognition capabilities. This study opens a new avenue for the systematic and predictable synthesis of PB nanoflowers, which broadens the repertoire of PBNPs for catalysis, biosensing, and imaging.

A multimode biosensor based on prussian blue nanoparticles loaded with gold nanoclusters for the detection of aflatoxin B1

“…PBNPs were synthesized according to the method described by Lu et al 28 Briefly, 5.4 mg of FeCl 3 ·6H 2 O and 107 mg of citric acid were added to a beaker containing 20 mL of deionized water. Then, 20 mL of deionized water containing 8.4 mg of K 4 [Fe(CN) 6 ] and 107 mg of citric acid was added dropwise to the above solution under vigorous stirring at 60 °C.…”

“…27 The multiple excellent properties of PBNPs as signal transducers have shown the great application potential of PBNPs in the eld of biosensing. [28][29][30] However, bare PBNPs have many drawbacks in application, such as difficulty in maintaining their dispersion in solution, and the issue that they are easily cleared by systemic circulation in biomedical applications.…”

Dual-Mode Lateral Flow Immunoassay Based on “Pompon Mum”-Like Fe3O4@MoS2@Pt Nanotags for Sensitive Detection of Viral Pathogens

“…40 Herein, a novel Fe 3 O 4 @MoS 2 @Pt composite with a unique "pompon mum" structure was designed. Overall, compared to the currently reported photothermal nanotags, the designed Fe 3 O 4 @MoS 2 @Pt composite not only retains a large surface area but also has a three-dimensional (3D) structure that is more conducive to the absorption of incident light, 41 achieving high photothermal performance. Specifically, as shown in Scheme 1, the Fe 3 O 4 as the core allows the vertical and regular growth of MoS 2 petals to expose more active edges as well as provides a fast magnetic separation ability, significantly simplifying the operation process.…”

Hollow Mesoporous Prussian Blue Nanoparticles for In Vivo Synergistic Chemo-Photothermal Cancer Therapy and Dual-Mode Magnetic Resonance/Fluorescence Imaging

“…Prussian blue (PB), an FDA-approved clinical drug used for the management of radiation-induced injuries, has been rigorously validated for its biosafety through accepted clinical diagnostics . The growing interest in Prussian Blue nanoparticles (PBNPs), owing to their intriguing characteristics and promising potential in biomedical applications, is gaining momentum. ,− The interconversion of Fe 3+ and Fe 2+ in PBNPs offers new oncology applications like photoacoustic tomography, magnetic resonance imaging, ultrasound imaging, and photothermal treatment. − Researchers aim to enhance PBNPs for clinical use, particularly in MRI and combined therapies. Boosting the longitudinal relaxivity of PBNPs remains a concern, and one effective approach involves doping high-spin transition metal ions such as Gd 3+ and Mn 2+ into the PB crystal structure.…”