DNA demethylation overcomes attenuation of colchicine biosynthesis in an endophytic fungus Diaporthe

Bhat, Deepika; Vohra, Manik; Mishra, Samita; Dorai, Kavita; Rai, Padmalatha; Satyamoorthy, Kapaettu; Murali, Thokur Sreepathy

doi:10.1016/j.jbiotec.2020.07.019

Cited by 9 publications

(5 citation statements)

References 53 publications

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“…It has already been described as a producer of enzymes and secondary metabolites 29 with antibacterial 30 , 31 and antifungal 32 , 33 properties as well as in the phytotoxicity for weeds control 34 – 37 . According to relevant reports, certain chemical compounds have been isolated and identified from this genus, such as camptothecin 38 and colchicine 39 . Some of the compounds produced showed herbicidal activity.…”

Section: Resultsmentioning

confidence: 99%

Physiological mechanism of action and partial separation of herbicide–active compounds from the Diaporthe sp. extract on Amaranthus tricolor L.

Manichart,

Laosinwattana,

Somala

et al. 2023

Sci Rep

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Thirteen fungi that produce compounds with herbicidal activities were isolated, identified, and extracted under the assumption that the mechanism of action occurs during seed exposure to the extract. The extracts from all the fungal strains considerably decreased the growth parameters of Amaranthus tricolor L. The EC010 strain extracts showed the greatest effect. Through ITS region gene sequencing methods, the isolated EC010 was identified as a genus of Diaporthe. The results showed a significant (p < 0.05) inhibitory effect of 91.25% on germination and a decrease in shoot and root length by 91.28% and 95.30%, respectively. The mycelium of Diaporthe sp. was extracted using sequential extraction techniques for the partial separation of the herbicidal fraction. According to the bioassay activities, the EtOAc fraction showed the highest inhibitory activity. The osmotic stress of the A. tricolor seeds was studied. Although the extract increased the accumulation of proline and soluble protein, the treated seeds showed lower imbibition. While the activity of α-amylase was dramatically decreased after treatment. A cytogenetic assay in the treated Allium cepa L. root revealed a decrease in the mitotic index, an altered mitotic phase index, and a promotion of mitotic abnormalities. Accordingly, the Diaporthe sp. may serve as a potential herbicidal compound resource.

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Section: Resultsmentioning

confidence: 99%

Physiological mechanism of action and partial separation of herbicide–active compounds from the Diaporthe sp. extract on Amaranthus tricolor L.

Manichart,

Laosinwattana,

Somala

et al. 2023

Sci Rep

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“…Endophytic fungus Diaporthe perseae from Gloriosa superba tubers was treated with 5-Aza at 1 μM. The production of colchicine ( 96 ) was found to be increased [ 66 ].…”

Section: Effects Of Dna Methyltransferase Modifiersmentioning

confidence: 99%

Recent Advances in Search of Bioactive Secondary Metabolites from Fungi Triggered by Chemical Epigenetic Modifiers

Xue

Hou

et al. 2023

JoF

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Genomic analysis has demonstrated that many fungi possess essential gene clusters for the production of previously unobserved secondary metabolites; however, these genes are normally reduced or silenced under most conditions. These cryptic biosynthetic gene clusters have become treasures of new bioactive secondary metabolites. The induction of these biosynthetic gene clusters under stress or special conditions can improve the titers of known compounds or the production of novel compounds. Among the inducing strategies, chemical-epigenetic regulation is considered a powerful approach, and it uses small-molecule epigenetic modifiers, which mainly act as the inhibitors of DNA methyltransferase, histone deacetylase, and histone acetyltransferase, to promote changes in the structure of DNA, histones, and proteasomes and to further activate cryptic biosynthetic gene clusters for the production of a wide variety of bioactive secondary metabolites. These epigenetic modifiers mainly include 5-azacytidine, suberoylanilide hydroxamic acid, suberoyl bishydroxamic acid, sodium butyrate, and nicotinamide. This review gives an overview on the method of chemical epigenetic modifiers to trigger silent or low-expressed biosynthetic pathways to yield bioactive natural products through external cues of fungi, mainly based on the research progress in the period from 2007 to 2022. The production of about 540 fungal secondary metabolites was found to be induced or enhanced by chemical epigenetic modifiers. Some of them exhibited significant biological activities such as cytotoxic, antimicrobial, anti-inflammatory, and antioxidant activity.

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“…Similarly, the addition of 1 µM of 5-azacytydine into the attenuated culture of the endophytic fungus Diaporthe perseae caused a significant increase in the colchicine production by 3.67-fold. The authors observed a gradual increase in the DNA methylation level on successive days of culturing, which was significantly reduced in the 5-azacytydine-treated culture [ 117 ]. Thus, endophytes that produce host-specific compounds require certain metabolic or molecular signaling, and in their absence, genes can be silenced by methylation.…”

Section: The Actual Source Of Plant Bioactive Moleculesmentioning

confidence: 99%

Plants as the Extended Phenotype of Endophytes—The Actual Source of Bioactive Compounds

Rutkowska

Drożdżyński

Ryngajłło

et al. 2023

IJMS

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For thousands of years, plants have been used for their medicinal properties. The industrial production of plant-beneficial compounds is facing many drawbacks, such as seasonal dependence and troublesome extraction and purification processes, which have led to many species being on the edge of extinction. As the demand for compounds applicable to, e.g., cancer treatment, is still growing, there is a need to develop sustainable production processes. The industrial potential of the endophytic microorganisms residing within plant tissues is undeniable, as they are often able to produce, in vitro, similar to or even the same compounds as their hosts. The peculiar conditions of the endophytic lifestyle raise questions about the molecular background of the biosynthesis of these bioactive compounds in planta, and the actual producer, whether it is the plant itself or its residents. Extending this knowledge is crucial to overcoming the current limitations in the implementation of endophytes for larger-scale production. In this review, we focus on the possible routes of the synthesis of host-specific compounds in planta by their endophytes.

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DNA demethylation overcomes attenuation of colchicine biosynthesis in an endophytic fungus Diaporthe

Cited by 9 publications

References 53 publications

Physiological mechanism of action and partial separation of herbicide–active compounds from the Diaporthe sp. extract on Amaranthus tricolor L.

Physiological mechanism of action and partial separation of herbicide–active compounds from the Diaporthe sp. extract on Amaranthus tricolor L.

Recent Advances in Search of Bioactive Secondary Metabolites from Fungi Triggered by Chemical Epigenetic Modifiers

Plants as the Extended Phenotype of Endophytes—The Actual Source of Bioactive Compounds

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