DNA damage response to different surface chemistry of silver nanoparticles in mammalian cells

Ahamed, Maqusood; Karns, Michael R.; Goodson, Michael S.; Rowe, John J.; Hussain, Saber M.; Schlager, John J.; Hong, Yan

doi:10.1016/j.taap.2008.09.015

Cited by 657 publications

(381 citation statements)

References 30 publications

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“…AgNPs are known to promote ROS production and DNA damage (26,53,54) which is consistent with their potential use as cancer therapeutics (29,55,56). However, as with most cytotoxic drugs, there are invariably off-target effects which restrict their use and our data indicates that AgNPs induce syncytin-1 expression in leukaemic cell lines.…”

Section: Discussionsupporting

confidence: 79%

Silver nanoparticles exhibit size-dependent differential toxicity and induce expression of syncytin-1 in FA-AML1 and MOLT-4 leukaemia cell lines

Alqahtani

Promtong

Oliver

et al. 2016

MUTAGE

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Human endogenous retrovirus (HERV) sequences make up approximately 8% of the human genome and increased expression of some HERV proteins has been observed in various pathologies including leukaemia and multiple sclerosis (MS). However, little is known about the function of these HERV proteins or environmental factors which regulate their expression. Silver nanoparticles (AgNPs) are used very extensively as antimicrobials and antivirals in numerous consumer products although their effect on the expression of HERV gene products is unknown. Cell proliferation and cell toxicity assays were carried out on human acute T lymphoblastic leukaemia (MOLT-4) and Fanconi anaemia associated acute myeloid leukaemia (FA-AML1) cells treated with two different sizes of AgNPs (7 nm and 50 nm diameter). RT-PCR and Western blotting were then used to the assess expression of HERV-W syncytin-1 mRNA and protein in these cells. FA-AML1 cells were more sensitive overall than MOLT-4 to treatment with the smaller 7 nm sized AgNp's being the most toxic in these cells. MOLT-4 cell were more resistant and showed no evidence of differential toxicity to the different sized particles. Syncytin-1 mRNA and protein were induced by both 7 and 50 nm AgNPs in both cell types yet with different kinetics. In summary, the observation that AgNPs induce expression of syncytin-1 in FA-AML1 and MOLT-4 cells at doses as little as 5 µg/ml is grounds for concern since this protein is up-regulated in both malignant and neurodegenerative diseases. Considering the widespread use of AgNPs in the environment it is clear that their ability to induce syncytin-1 should be investigated further in other cell types.3

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Section: Discussionsupporting

confidence: 79%

Silver nanoparticles exhibit size-dependent differential toxicity and induce expression of syncytin-1 in FA-AML1 and MOLT-4 leukaemia cell lines

Alqahtani

Promtong

Oliver

et al. 2016

MUTAGE

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“…Many studies have reported the apoptosis induced by nanoparticles. In vitro studies indicated that silver nanoparticles caused apoptosis in mouse embryonic stem cells [38]. In another investigation, the release of apoptosis markers viz.…”

Section: Apoptosis Assaymentioning

confidence: 95%

In vitro and in vivo toxicity assessment of nanoparticles

2017

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Nanotechnology has revolutionized gene therapy, diagnostics and environmental remediation. Their bulk production, uses and disposal have posed threat to the environment. With the appearance of these nanoparticles in the environment, their toxicity assessment is an immediate concern. This review is an attempt to summarize the major techniques used in cytotoxity determination. The review also presents a detailed and elaborative discussion on the toxicity imposed by different types of nanoparticles including carbon nanotubes, gold nanoparticles, silver nanoparticles, quantum dots, fullerenes, aluminium nanoparticles, zinc nanoparticles, iron nanoparticles, titanium nanoparticles and silica nanoparticles. It discusses the in vitro and in vivo toxological effects of nanoparticles on bacteria, microalgae, zebrafish, crustacean, fish, rat, mouse, pig, guinea pig, human cell lines and human. It also discusses toxological effects on organs such as liver, kidney, spleen, sperm, neural tissues, liver lysosomes, spleen macrophages, glioblastoma cells, hematoma cells and various mammalian cell lines. It provides information about the effects of nanoparticles on the gene-expression, growth and reproduction of the organisms.

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“…For instance, different surface chemistries of Ag NPs with similar sizes (25 nm) are reported to induce DNA damage in mouse embryonic stem cells and mouse embryonic fibroblasts [49]. In particular, the authors observed that polysaccharide-coated Ag NPs exert higher DNA damage than the uncoated particles by measurements of the double strand breaks repair protein Rad51.…”

Section: Nanoparticlesmentioning

confidence: 99%

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Broggi¹,

Ponti²,

Giudetti

et al. 2013

BioNanoMaterials

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Silver nanoparticles (Ag NPs) are one of the most common nanomaterials present in nanotechnologybased products. Here, the physical chemical properties of Ag NPs suspensions of 44 nm, 84 nm and 100 nm sizes synthesized in our laboratory were characterized. The NM-300 material (average size of 17 nm), supplied by the Joint Research Centre Nanomaterials Repository was also included in the present study. The Ag NPs potential cytotoxicity was tested on the Balb3T3 cell line by the Colony Forming Efficiency assay, while their potential morphological neoplastic transformation and genotoxicity were tested by the Cell Transformation Assay and the micronucleus test, respectively. After 24 h of exposure, NM-300 showed cytotoxicity with an IC50 of 8 µM (corresponding to 0.88 µg/mL) while for the other nanomaterials tested, values of IC50 were higher than 10 µM (1.10 µg/mL). After 72 h of exposure, Ag NPs showed size-dependent cytotoxic effect with IC50 values of 1.5 µM (1.16 µg/mL) for NM-300, 1.7 µM (1.19 µg/mL) for Ag 44 nm, 1.9 µM (0.21 µg/mL) for Ag 84 nm and 3.2 µM (0.35 µg/mL) for Ag 100 nm. None of the Ag NPs tested was able to induce either morphological neoplastic transformation or micronuclei formation.

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DNA damage response to different surface chemistry of silver nanoparticles in mammalian cells

Cited by 657 publications

References 30 publications

Silver nanoparticles exhibit size-dependent differential toxicity and induce expression of syncytin-1 in FA-AML1 and MOLT-4 leukaemia cell lines

Silver nanoparticles exhibit size-dependent differential toxicity and induce expression of syncytin-1 in FA-AML1 and MOLT-4 leukaemia cell lines

In vitro and in vivo toxicity assessment of nanoparticles

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

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