DNA Damage Potential of Engine Emissions Measured <i>In Vitro</i> by Micronucleus Test in Human Bronchial Epithelial Cells

Červená, Tereza; Rössnerová, Andrea; Sikorová, Jitka; Beránek, V.; Vojtíšek-Lom, Michal; Cigánek, Miroslav; Topinka, Jan; Rössner, Pavel

doi:10.1111/bcpt.12693

Cited by 27 publications

(12 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As for vehicular traffic, diesel exhaust particulate material was recognized to induce MN formation in human bronchial epithelial cells (BEAS-2B) [69]. Some in vivo studies correlated micronuclei with residence in intense traffic areas, showing that cytogenetic damage was even higher in children than in adults [25,61].…”

Section: Discussionmentioning

confidence: 99%

Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy)

Panìco

Grassi

Bagordo

et al. 2020

IJERPH

View full text Add to dashboard Cite

Micronuclei (MN) are biomarkers of early biological effect often used for detecting DNA damage in human population exposed to genotoxic agents. The aim of this study was to evaluate the frequency of MN in exfoliated buccal cells of children living in an industrialized (impacted) area compared with that found in children living in a control area without significant anthropogenic impacts. A total of 462 6–8-year-old children (206 in the impacted area, 256 in the control area) attending primary school were enrolled. A questionnaire was administered to the parents of the recruited children to obtain information about personal data, lifestyles, and food habits of their children. Atmospheric particulate fractions were collected near the involved schools to assess the level of environmental exposure of the children. The presence of MN was highlighted in 68.4% of children living in the impacted area with a mean MN frequency of 0.66‰ ± 0.61‰. MN positivity and frequency were significantly lower in the control area (37.1% and 0.27‰ ± 0.43‰, respectively). The frequency of MN was positively associated with quasi-ultrafine particulate matter (PM0.5), traffic near the home, and consuming barbecued food; while adherence to the Mediterranean diet and practicing sport were negatively associated.

show abstract

Section: Discussionmentioning

confidence: 99%

Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy)

Panìco

Grassi

Bagordo

et al. 2020

IJERPH

View full text Add to dashboard Cite

show abstract

“…The cell models were grown at ALI at 37 °C, 5% CO 2 , and relative humidity >90% in 24-well format Transwell ® cell culture inserts (Sigma-Aldrich, St Louis, MO, USA). For BEAS-2B cells, serum-free cultivation conditions (BEGM™ kit CC- 3170; Lonza, Basel, Switzerland) were used and 100,000 cells/insert were seeded [40]. Twenty-four hours after seeding, the apical medium was removed from the insert and the cells were kept 24 h at ALI conditions prior to exposure.…”

Section: Methodsmentioning

confidence: 99%

The Biological Effects of Complete Gasoline Engine Emissions Exposure in a 3D Human Airway Model (MucilAirTM) and in Human Bronchial Epithelial Cells (BEAS-2B)

Rössner

Červená

Vojtíšek-Lom

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

The biological effects induced by complete engine emissions in a 3D model of the human airway (MucilAirTM) and in human bronchial epithelial cells (BEAS-2B) grown at the air–liquid interface were compared. The cells were exposed for one or five days to emissions generated by a Euro 5 direct injection spark ignition engine. The general condition of the cells was assessed by the measurement of transepithelial electrical resistance and mucin production. The cytotoxic effects were evaluated by adenylate kinase (AK) and lactate dehydrogenase (LDH) activity. Phosphorylation of histone H2AX was used to detect double-stranded DNA breaks. The expression of the selected 370 relevant genes was analyzed using next-generation sequencing. The exposure had minimal effects on integrity and AK leakage in both cell models. LDH activity and mucin production in BEAS-2B cells significantly increased after longer exposures; DNA breaks were also detected. The exposure affected CYP1A1 and HSPA5 expression in MucilAirTM. There were no effects of this kind observed in BEAS-2B cells; in this system gene expression was rather affected by the time of treatment. The type of cell model was the most important factor modulating gene expression. In summary, the biological effects of complete emissions exposure were weak. In the specific conditions used in this study, the effects observed in BEAS-2B cells were induced by the exposure protocol rather than by emissions and thus this cell line seems to be less suitable for analyses of longer treatment than the 3D model.

show abstract

“…Authors found that double strand breaks were induced within the BEAS-2B cells through use of the γ-H2AX assay [73]. A similar study found that engine emission exposures increased MN frequency in BEAS-2B cells [129]. Such indicators of genotoxicity are important to evaluate, as genotoxicity represents a potential key molecular event involved in chemical-induced carcinogenesis and other disease phenotypes [130,131].…”

Section: Gene-level Changesmentioning

confidence: 98%

New Approach Methods to Evaluate Health Risks of Air Pollutants: Critical Design Considerations for In Vitro Exposure Testing

Zavala¹,

Freedman

Szilagyi

et al. 2020

IJERPH

View full text Add to dashboard Cite

Air pollution consists of highly variable and complex mixtures recognized as major contributors to morbidity and mortality worldwide. The vast number of chemicals, coupled with limitations surrounding epidemiological and animal studies, has necessitated the development of new approach methods (NAMs) to evaluate air pollution toxicity. These alternative approaches include in vitro (cell-based) models, wherein toxicity of test atmospheres can be evaluated with increased efficiency compared to in vivo studies. In vitro exposure systems have recently been developed with the goal of evaluating air pollutant-induced toxicity; though the specific design parameters implemented in these NAMs-based studies remain in flux. This review aims to outline important design parameters to consider when using in vitro methods to evaluate air pollutant toxicity, with the goal of providing increased accuracy, reproducibility, and effectiveness when incorporating in vitro data into human health evaluations. This review is unique in that experimental considerations and lessons learned are provided, as gathered from first-hand experience developing and testing in vitro models coupled to exposure systems. Reviewed design aspects include cell models, cell exposure conditions, exposure chambers, and toxicity endpoints. Strategies are also discussed to incorporate in vitro findings into the context of in vivo toxicity and overall risk assessment.

show abstract

DNA Damage Potential of Engine Emissions Measured In Vitro by Micronucleus Test in Human Bronchial Epithelial Cells

Cited by 27 publications

References 23 publications

Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy)

Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy)

The Biological Effects of Complete Gasoline Engine Emissions Exposure in a 3D Human Airway Model (MucilAirTM) and in Human Bronchial Epithelial Cells (BEAS-2B)

New Approach Methods to Evaluate Health Risks of Air Pollutants: Critical Design Considerations for In Vitro Exposure Testing

Contact Info

Product

Resources

About