DNA damage of human spermatozoa in assisted reproduction: origins, diagnosis, impacts and safety

Özmen, Batuhan; Koutlaki, N; Youssry, Mohamed; Diedrich, K.; Al‐Hasani, S.

doi:10.1016/s1472-6483(10)60883-8

Cited by 75 publications

(60 citation statements)

References 108 publications

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“…During epididymal maturation, the protamine disulfide cross linking is completed, confering a highly compact structure to sperm chromatin. If the disulphide cross linking is incomplete it may lead to suboptimal compaction and therefore high degree of DNA fragmentation [47]. DNA damage incurred during sperm transit and storage in epididymis or post ejaculation cannot be repaired by sperm because post spermiogenesis there is negligible transcription and translation [28,48].…”

Section: Aetiology Of Sperm Dna Damagementioning

confidence: 99%

Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility

Shamsi

Imam

Dada

2011

J Assist Reprod Genet

135

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Sperm is not a simple carrier of paternal genetic information but its role extends clearly beyond fertilization. Integrity of sperm genome is an essential pre-requisite for birth of healthy offspring and evaluation of sperm should entail DNA integrity analysis. DNA integrity analysis is a better diagnostic and prognostic marker of sperm reproductive potential. Conventional semen analysis emphasizes on sperm concentration, viability, motility and morphology and has been proven to be a poor indicator of reproductive potential and pregnancy outcome. To overcome the drawbacks associated with conventional semen analysis more useful fertility tests and molecular biomarkers have been explored. Among the different tests which have evolved for assessing the sperm reproductive potential, tests for sperm DNA quality are most promising. Sperm DNA damage has been closely associated with numerous indicators of reproductive health including fertilization, embryo quality, implantation, spontaneous abortion, congenital malformations and childhood diseases. It therefore has great potential as a prognostic test for both in vitro and in vivo conception. This review presents an updated account of tests that have better diagnostic and prognostic implications in the evaluation of sperm DNA damage. The basic principles, outline of methodology, advantage, disadvantage, clinical significance of each technique and implications of these tests have been discussed. The logistics of each test with respect to available resources and equipment in an andrology laboratory, the feasibility of performing these tests in routine diagnostic workup of infertile men and the opportunities and challenges provided by DNA testing in male fertility determination are also presented.

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Section: Aetiology Of Sperm Dna Damagementioning

confidence: 99%

Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility

Shamsi

Imam

Dada

2011

J Assist Reprod Genet

135

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“…This possibility presents important consequences for a range of basic research into sperm DNA degradation, which remains poorly understood, even in humans (Tesarik et al 2006, Ozmen et al 2007. For example, the consequences of single-or double-stranded sperm DNA breakage for fertilization or embryogenesis remain unknown, although recognized as potentially important (Genescá et al 1992, Khanna & Jackson 2001, Zhang et al 2007, and recognized in some recent studies (Gandini et al 2004, Payne et al 2005.…”

Section: T0mentioning

confidence: 99%

Rapid rates of sperm DNA damage after activation in tench (Tinca tinca: Teleostei, Cyprinidae) measured using a sperm chromatin dispersion test

López‐Fernández¹,

Gage²,

Arroyo³

et al. 2009

Reproduction

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Spermatozoal haplotypic DNA is prone to damage, leading to male fertility problems. So far, the assessment of sperm DNA breakage has been challenging because protamines render the nuclear chromatin highly compacted. Here, we report the application of a new test to quantify DNA fragmentation in spermatozoa of an externally fertilizing teleost fish. The sperm chromatin dispersion (SCD) test uses a species-specific lysing solution to generate controlled protein depletion that, followed by DNA-specific fluorescent labelling, allows an easy morphological discrimination between nuclei affected by DNA damage. Using tench (Tinca tinca) as our model, we first trialled the test against established, but more technically demanding, assays employing in situ nick translation (ISNT) and the comet assay. The SCD test showed high concordance with ISNT, comet assay measures and a chromatin-swelling test, confirming the application of this straightforward SCD technique to various aspects of reproductive biology. Second, we examined between-male variation in DNA damage, and measured changes through time following spermatozoal activation. Between-male variation in the basal levels of average DNA damage ranged from 0 to 20% of sperm showing damage, and all showed increases in DNA fragmentation through time (0-60 min). The rates of DNA damage increase are the fastest so far recorded in sperm for a living organism, and may relate to the external fertilization mode. Our findings have relevance for broodstock selection and optimizing IVF protocols routinely used in modern aquaculture.

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“…Акридин оранжевый определяет тиол-дисульфидный статус клеточного ядра [62] и ме-няет цвет с красного на зеленый по мере созревания сперматозоидов [63]. Широко используется анилиновый синий, окрашивающий гистоны [64]. Хромомицин А3 относится к природным антибиотикам и связывается с Г-Ц участками ДНК, отражая статус протаминизации [65,66].…”

Section: корреляционная электронная микроскопияunclassified

Abnormal Chromatin Condensation in Spermatozoa and Dna Fragmentation in Spermatozoa: Is There a Correlation?

Брагина¹,

Arifulin²,

Лазарева³

et al. 2017

Androl. genit. hir.

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DNA damage of human spermatozoa in assisted reproduction: origins, diagnosis, impacts and safety

Cited by 75 publications

References 108 publications

Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility

Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility

Rapid rates of sperm DNA damage after activation in tench (Tinca tinca: Teleostei, Cyprinidae) measured using a sperm chromatin dispersion test

Abnormal Chromatin Condensation in Spermatozoa and Dna Fragmentation in Spermatozoa: Is There a Correlation?

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