DNA damage-induced autophagy is regulated by inositol polyphosphate synthetases in Candida albicans

Du, Jiawen; Dong, Yixuan; Zhu, Hangqi; Deng, Ying; Sa, Chula; Yu, Qilin; Li, Mingchun

doi:10.1016/j.bbamcr.2023.119622

Cited by 1 publication

(6 citation statements)

References 73 publications

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“…However, the atg17∆/∆ strains exhibited weak autophagy under both conditions and seemed to show even weaker autophagy under nitrogen starvation (Figure 1), indicating that the importance of Atg17 in the two types of autophagy is different. The autophagy of these strains under an MMS treatment has been examined in our previous study [40], and the function of Atg1 and Atg11 on macroautophagy has also been identified [46,47]. However, in order to compare the differences between the two types of autophagy more intuitively, we repeated the experiment, and the results were consistent with our previous work [40,46,47].…”

Section: Comparison Of the Role Of Atg Proteins In Dna Damage-induced...supporting

confidence: 80%

“…MMS treatment has been examined in our previous study [40], and the function of Atg1 and Atg11 on macroautophagy has also been identified [46,47]. However, in order to compare the differences between the two types of autophagy more intuitively, we repeated the experiment, and the results were consistent with our previous work [40,46,47].…”

Section: The Domains Of Atg13 Play An Essential Role In Dna Damage-in...supporting

confidence: 73%

“…DNA damage has been shown to induce autophagy in mammalian cells and yeast [21,22], and in our previous work, we also found that DNA damage can induce autophagy in C. albicans [40]. The traditional and best-studied autophagy is macroautophagy, also known as non-selective autophagy [41], which is usually activated under nitrogen starvation conditions [42].…”

Section: Comparison Of the Role Of Atg Proteins In Dna Damage-induced...mentioning

confidence: 73%

“…The autophagy of these strains under an MMS treatment has been examined in our previous study [40], and the function of Atg1 and Atg11 on macroautophagy has also been identified [46,47]. However, in order to compare the differences between the two types of autophagy more intuitively, we repeated the experiment, and the results were consistent with our previous work [40,46,47]. These data indicated that the core Atg proteins (Atg1, Atg11, Atg13, and Atg18) have essential roles in DNA damage-induced autophagy and macroautophagy, while the function of Atg17 is slightly different between the two types of autophagy.…”

Section: Comparison Of the Role Of Atg Proteins In Dna Damage-induced...mentioning

confidence: 99%

“…We have proven that DNA damage caused by MMS can induce autophagy in C. albicans [40], and DNA also exists in the mitochondria [51]. In S. cerevisiae, Om45 is a mitochondrial matrix protein, and its degradation can be used to represent the degree of mitophagy [52].…”

Section: Dna Damage Failed To Induce Mitophagy and Er Autophagymentioning

confidence: 99%

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Mec1-Rad53 Signaling Regulates DNA Damage-Induced Autophagy and Pathogenicity in Candida albicans

Du,

Dong,

Zuo

et al. 2023

JoF

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DNA damage activates the DNA damage response and autophagy in C. albicans; however, the relationship between the DNA damage response and DNA damage-induced autophagy in C. albicans remains unclear. Mec1-Rad53 signaling is a critical pathway in the DNA damage response, but its role in DNA damage-induced autophagy and pathogenicity in C. albicans remains to be further explored. In this study, we compared the function of autophagy-related (Atg) proteins in DNA damage-induced autophagy and traditional macroautophagy and explored the role of Mec1-Rad53 signaling in regulating DNA damage-induced autophagy and pathogenicity. We found that core Atg proteins are required for these two types of autophagy, while the function of Atg17 is slightly different. Our results showed that Mec1-Rad53 signaling specifically regulates DNA damage-induced autophagy but has no effect on macroautophagy. The recruitment of Atg1 and Atg13 to phagophore assembly sites (PAS) was significantly inhibited in the mec1Δ/Δ and rad53Δ/Δ strains. The formation of autophagic bodies was obviously affected in the mec1Δ/Δ and rad53Δ/Δ strains. We found that DNA damage does not induce mitophagy and ER autophagy. We also identified two regulators of DNA damage-induced autophagy, Psp2 and Dcp2, which regulate DNA damage-induced autophagy by affecting the protein levels of Atg1, Atg13, Mec1, and Rad53. The deletion of Mec1 or Rad53 significantly reduces the ability of C. albicans to systematically infect mice and colonize the kidneys, and it makes C. albicans more susceptible to being killed by macrophages.

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Section: Comparison Of the Role Of Atg Proteins In Dna Damage-induced...supporting

confidence: 80%

Section: The Domains Of Atg13 Play An Essential Role In Dna Damage-in...supporting

confidence: 73%

Section: Comparison Of the Role Of Atg Proteins In Dna Damage-induced...mentioning

confidence: 73%

Section: Comparison Of the Role Of Atg Proteins In Dna Damage-induced...mentioning

confidence: 99%

Section: Dna Damage Failed To Induce Mitophagy and Er Autophagymentioning

confidence: 99%

See 3 more Smart Citations

Mec1-Rad53 Signaling Regulates DNA Damage-Induced Autophagy and Pathogenicity in Candida albicans

Du,

Dong,

Zuo

et al. 2023

JoF

Self Cite

View full text Add to dashboard Cite

show abstract

DNA damage-induced autophagy is regulated by inositol polyphosphate synthetases in Candida albicans

Cited by 1 publication

References 73 publications

Mec1-Rad53 Signaling Regulates DNA Damage-Induced Autophagy and Pathogenicity in Candida albicans

Mec1-Rad53 Signaling Regulates DNA Damage-Induced Autophagy and Pathogenicity in Candida albicans

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