DNA damage in peripheral blood lymphocytes and association with polymorphisms in the promoter region of the CYP2E1 gene in alcoholics from Central Brazil

Ramos, Jheneffer Sonara Aguiar; Alves, Alessandro Arruda; Lopes, Mariana Paiva; Pedroso, Thays Millena Alves; Felício, Leandro Prado; Carvalho, Wanessa Fernandes; Franco, Fernanda Craveiro; Melo, C.; Gonçalves, Macks Wendhell; Soares, Thannya Nascimento; Cruz, Aparecido Divino da; Silva, Daniela de Melo e

doi:10.1016/j.alcohol.2016.08.007

Cited by 12 publications

(4 citation statements)

References 17 publications

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“…Guang et al 33 found that the CYP2E1 rs3813867 mutant allele was associated with higher micronuclei among benzene‐exposed shoe workers. Jheneffer et al 34 reported that alcoholics who heterozygous in the CYP2E1 rs3813867 showed higher DNA damage (tail length and olive tail moment). Jing et al 35 found heterozygous in the CYP2E1 rs6413432 had shorter telomere lengths among benzene‐exposed shoe workers.…”

Section: Discussionmentioning

confidence: 99%

Genetic polymorphisms of metabolic enzyme genes associated with leukocyte mitochondrial DNA copy number in PAHs exposure workers

Duan

Zhang

et al. 2021

Cancer Reports

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Background: Polycyclic aromatic hydrocarbons (PAHs) exposure had been reported to be a risk factor of mtDNAcn in our early study. However, the effect of metabolic enzymes' genetic polymorphisms on mtDNAcn in PAHs-Exposure workers has not been fully evaluated.Aim: The aim of the study was to explore the effect of metabolic enzymes' genetic polymorphisms on mtDNAcn in PAHs-Exposure. Methods and Results:We investigated the effects of metabolic enzymes' genetic polymorphisms on mtDNAcn among 544 coke oven workers and 238 office staffs.The mtDNAcn of peripheral blood leukocytes was measured using the Real-time quantitative polymerase chain reaction (PCR) method. PCR and restriction fragment length was used to detect five polymorphisms in GSTT1, GSTM1, GSTP1 rs1695, CYP2E1 rs6413432, and CYP2E1 rs3813867. The mtDNAcn in peripheral blood leukocytes was significantly lower in the exposure group than that in the control group (p < .001). The 1-OHPYR had an increasing trend with the genotypes AA!AG ! GG of GSTP1 rs1695 in the control group. Generalized linear model indicated that the influencing factors of mtDNAcn were PAHs-exposure [β (95% CI) = −0.420 (−0.469, −0.372), p < .001], male [β (95% CI) = −0.058 (−0.103, −0.012), p = .013], and AA genotype for GSTP1 rs1695 [β (95% CI) = −0.051 (−0.095, −0.008), p = .020]. Conclusion:The individuals carrying the AA genotype of GSTP1 rs1695 may have a lower mtDNAcn due to their weaker detoxification of PAHs.

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Section: Discussionmentioning

confidence: 99%

Genetic polymorphisms of metabolic enzyme genes associated with leukocyte mitochondrial DNA copy number in PAHs exposure workers

Duan

Zhang

et al. 2021

Cancer Reports

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“…Guang et al [30] found that CYP2E1 rs3813867 mutant allele was associated with higher micronuclei among benzeneexposed shoe workers. Jheneffer et al [31] reported that alcoholics who heterozygous in the CYP2E1 rs3813867 showed higher DNA damage (tail length and olive tail moment). Jing et al [32]found heterozygous in the CYP2E1 rs6413432 had shorter telomere lengths among benzene-exposed shoe workers.…”

Section: Gstt1 Gstm1mentioning

confidence: 99%

Genetic Polymorphisms of Metabolic Enzyme Genes Associated with Leukocyte Mitochondrial DNA Copy Number in PAHs Exposure Workers

Duan

Zhang

et al. 2020

Preprint

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Background: PAHs exposure had been reported to be a risk factor of mtDNAcn in our early study. However, the effect of metabolic enzymes’ genetic polymorphisms on mtDNAcn in PAHs-Exposure workers has not been fully evaluated.Methods: We investigated the effects of metabolic enzymes’ genetic polymorphisms on mtDNAcn among 544 coke oven workers and 238 office staffs. The mtDNAcn of peripheral blood leukocytes was measured using Real-time quantitative polymerase chain reaction method. Polymerase chain reaction and restriction fragment length was used to detect five polymorphisms in GSTT1, GSTM1, GSTP1 rs1695, CYP2E1 rs6413432, and CYP2E1 rs3813867.Results: The mtDNAcn in peripheral blood leukocytes was significantly lower in the exposure group than that in the control group (P < 0.001). The 1-OHPYR had an increasing trend with the genotypes AA→AG→GG of GSTP1 rs1695 in the control group. Generalized linear model indicated that the influencing factors of mtDNAcn were PAHs-exposure [b(95% CI) = -0.420 (-0.469, -0.372), P < 0.001], male [β(95% CI) = -0.058 (-0.103, -0.012), P = 0.013] ,and AA genotype for GSTP1 rs1695 [β(95% CI) = -0.051 (-0.095, -0.008), P = 0.020].Conclusions: The male was susceptibility to PAHs exposure. The AA genotype of GSTP1 rs1695 may influence the toxicity of PAHs and associated with the decreased of mtDNAcn.

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“…Exposure to agricultural pesticides can lead to the impairment of a set of essential functions, such as the inhibition of carboxyl ester hydrolases, chymotrypsin, acetylcholinesterase (AchE), butyrylcholinesterase, liver carboxylases, and paraoxonases (Abdollahi et al, 2004;Kampire et al, 2011;Sifuentes Dos Santos et al, 2015). Exposure may also cause, directly or indirectly, damage to the single-or double-stranded DNA by producing Reactive Oxygen Species, or ROS (Ceja Galvez et al, 2021;Franco et al, 2016;Godoy et al, 2014;Rohr et al, 2011;. Base Excision Repair (BER) is a fundamental pathway of repairing single-stranded DNA breaks, which may help to minimize this type of impact caused by pesticides (Ceja Galvez et al, 2021;de Oliveira et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…also found an association between the A allele and increased DNA damage in the sperm in a Chinese study of 1,657 men suffering from infertility.These associations may re ect the fact that the A allele produces isoforms with more intense activity(Rossignol et al, 2014;Siller-López et al, 2017;Sridon et al, 2020). The more active isoform generates more genotoxic and carcinogenic metabolites which, in turn, may not be biotransformed entirely in the second stage of liver detoxi cation Ramos et al (2016),. for example, found that the alleles that cause the over-expression of CYP2E1 lead to an increase in enzyme activity by forming more genotoxic metabolites and increasing DNA damage, as indicated by the evaluation polymorphisms of the CPY2E1 gene in alcoholics from Goiás state.…”

mentioning

confidence: 99%

The Incidence of the XRCC1 rs25487 and PON1 rs662 Polymorphisms in a Population from Central Brazil: Patterns in an Area with a High Level of Agricultural Activity

et al. 2023

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In Brazil, high levels of agricultural activity are re ected in the consumption of enormous amounts of pesticide. The production of grain in Brazil has been estimated to reach 289.8 million tons in the 2022 harvest, an increase of 14.7% in comparison with 2021, with an even greater increase being predicted for Goiás, one of the country's principal grain producing states. These advances are likely associated with a progressive increase in the occupational exposure of rural workers to pesticides. The PON1 gene activates the enzymes involved in phase I of liver detoxi cation. The rs662 variant of this gene is known to modify the activity of the PON1 enzyme, which can increase the susceptibility of an individual to pesticides, leading to more severe symptoms or the development of diseases. The present study describes the frequencies of the rs25487 (of the XRCC gene) and rs662 variants and their haplotypes in a sample population from Goiás, Brazil, and compares the frequencies with other populations from around the world to verify the variation in the distribution of these Single Nucleotide Polymorphisms (SNPs). The study analyzed samples taken from 494 unrelated individuals resident in the state of Goiás. The A allele of the rs25487 variant had a frequency of 26% in the Goiás study population, and the modi ed rs662 G allele had a frequency of 42.8%. Four haplotypes were recorded for the rs25487 (G > A) and rs662 (A > G) markers, with a frequency of 11.9% being recorded for the A/G haplotype (both modi ed alleles), 30.8% for the G/G haplotype, 14.3% for the A/A haplotype, and 42.8% for the G/A haplotype (both wild-type alleles). In conclusion, we demonstrated the distribution of important SNPs associated with pesticide exposure in an area that presents a high level of agricultural activity.

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DNA damage in peripheral blood lymphocytes and association with polymorphisms in the promoter region of the CYP2E1 gene in alcoholics from Central Brazil

Cited by 12 publications

References 17 publications

Genetic polymorphisms of metabolic enzyme genes associated with leukocyte mitochondrial DNA copy number in PAHs exposure workers

Genetic polymorphisms of metabolic enzyme genes associated with leukocyte mitochondrial DNA copy number in PAHs exposure workers

Genetic Polymorphisms of Metabolic Enzyme Genes Associated with Leukocyte Mitochondrial DNA Copy Number in PAHs Exposure Workers

The Incidence of the XRCC1 rs25487 and PON1 rs662 Polymorphisms in a Population from Central Brazil: Patterns in an Area with a High Level of Agricultural Activity

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