DNA damage in peripheral blood mononuclear cells of patients undergoing anti-tuberculosis treatment

Oliveira, Larissa Ragozo Cardoso de; Peresi, Eliana; Tavares, Francilene Capel; Corrêa, Camila Renata; Pierine, Damiana Tortolero; Calvi, Sueli Aparecida

doi:10.1016/j.mrgentox.2012.04.003

Cited by 11 publications

(5 citation statements)

References 31 publications

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“…The results of the present work show that cells from infected animals present DNA damage similarly to other studies reporting that microorganisms such as Trypanosoma cruzi , Opisthorchis viverrini , Leishmania chagasi , Toxoplasma gondii , and Mycobacterium tuberculosis can induce DNA damage [ 14 , 17 - 19 , 23 ]. Some studies have demonstrated that certain fungi – such as Stachybotrys chartarum, Aspergillus versicolor, Fusarium mycotoxin, Saccharomyces cerevisiae and Aspergillus flavus – also have the potential to damage DNA [ 24 - 27 ].…”

Section: Discussionsupporting

confidence: 88%

DNA damage in BALB/c mice infected with Lacazia loboi and its relation to nutritional status

Barbosa

Oliveira

Tavares

et al. 2015

J Venom Anim Toxins Incl Trop Dis

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BackgroundJorge Lobo’s disease, also known as lacaziosis, is a cutaneous-subcutaneous mycosis with chronic evolution. It is caused by the fungus Lacazia loboi. Herein we report a study that relates the genotoxicity caused by L. loboi in isogenic mice with nutritional status, through a normal or restricted diet.MethodsDNA damage was assessed in the peripheral blood by the comet assay (tail intensity).ResultsThe results for leukocytes showed increases in the mean tail intensity in mice under dietary restriction, in infected mice under dietary restriction and in infected mice ingesting a normal diet.ConclusionThese results indicate that dietary restriction and L. loboi infection may increase DNA damage levels in mice, as detected by the comet assay.

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Section: Discussionsupporting

confidence: 88%

DNA damage in BALB/c mice infected with Lacazia loboi and its relation to nutritional status

Barbosa

Oliveira

Tavares

et al. 2015

J Venom Anim Toxins Incl Trop Dis

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“…The present work is an attempt to report the ROS-induced perturbation in these immune cells at molecular level. Earlier studies have already shown that active MTB infection is positively associated with oxidative stress and DNA damage (de Oliveira et al, 2012;Selek et al, 2012). It has been also reported that the genetic alterations caused by MTB infection could be linked to immune alterations and, perhaps, increased susceptibility to cancer (Rao et al, 1990).…”

Section: Discussionmentioning

confidence: 99%

Role of mitochondrial oxidative stress on lymphocyte homeostasis in patients diagnosed with extra‐pulmonary tuberculosis

Bhargava

Khare

Bunkar

et al. 2015

Cell Biology International

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Extra-pulmonary tuberculosis is often an underrated illness. Recent clinical studies have pointed out that lymphocyte homeostasis is dramatically disturbed as revealed through a series of signs and symptoms. Lymphocytes, the known effector cells of our immune system, play an important role in providing immunologic resistance against Mycobacterium infection. It is important to have quantitative insights into the lifespan of these cells; therefore, we aimed to study the precise effect of gastrointestinal tuberculosis infection on peripheral blood lymphocyte subpopulations and function. Our results indicated that gastrointestinal tuberculosis could increase mitochondrial oxidative stress, lower mitochondrial DNA copy number, promote nuclear DNA damage and repair response, decrease mitochondrial respiratory chain enzyme activities, and upregulate Bcl-2 and caspase-3 gene expression in lymphocytes. We further revealed that Mycobacterium infection induces autophagy for selective sequestration and subsequent degradation of the dysfunctional mitochondrion before activating cellular apoptosis in the peripheral lymphocyte pool. Together, these observations uncover a new role of mitochondrial-nuclear crosstalk that apparently contributes to lymphocyte homeostasis in gastrointestinal tuberculosis infection.

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“…Using a series of experiments in a mouse model, Mohanty et al (2016) showed that M. tuberculosis -infected macrophages produce genomic instability associated with high levels of NO and ROI. In contrast, de Oliveira et al (2012) studied lymphocytes of patients with pulmonary TB and failed to link DNA damage with iNOS gene expression, which is fundamental for nitric oxide production. However, DNA damage was suggested to be associated with other possible free radicals and oxidants (superoxide anion, nitrogen dioxide, H 2 O 2 , and hypochlorous acid).…”

Section: Discussionmentioning

confidence: 99%

Mycobacterium tuberculosis promotes genomic instability in macrophages

Castro-Garza

Luévano-Martínez

Villarreal-Treviño

et al. 2018

Mem. Inst. Oswaldo Cruz

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BACKGROUND Mycobacterium tuberculosis is an intracellular pathogen, which may either block cellular defensive mechanisms and survive inside the host cell or induce cell death. Several studies are still exploring the mechanisms involved in these processes.OBJECTIVESTo evaluate the genomic instability of M. tuberculosis-infected macrophages and compare it with that of uninfected macrophages.METHODSWe analysed the possible variations in the genomic instability of Mycobacterium-infected macrophages using the DNA breakage detection fluorescence in situ hybridisation (DBD-FISH) technique with a whole human genome DNA probe.FINDINGSQuantitative image analyses showed a significant increase in DNA damage in infected macrophages as compared with uninfected cells. DNA breaks were localised in nuclear membrane blebs, as confirmed with DNA fragmentation assay. Furthermore, a significant increase in micronuclei and nuclear abnormalities were observed in infected macrophages versus uninfected cells.MAIN CONCLUSIONSGenomic instability occurs during mycobacterial infection and these data may be seminal for future research on host cell DNA damage in M. tuberculosis infection.

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DNA damage in peripheral blood mononuclear cells of patients undergoing anti-tuberculosis treatment

Cited by 11 publications

References 31 publications

DNA damage in BALB/c mice infected with Lacazia loboi and its relation to nutritional status

DNA damage in BALB/c mice infected with Lacazia loboi and its relation to nutritional status

Role of mitochondrial oxidative stress on lymphocyte homeostasis in patients diagnosed with extra‐pulmonary tuberculosis

Mycobacterium tuberculosis promotes genomic instability in macrophages

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