DNA damage in human fibroblasts exposed to fumonisin B1

Galvano, Fabio; Russo, Alessandra; Cardile, Venera; Galvano, G.; Vanella, A.; Renis, Marcella

doi:10.1016/s0278-6915(01)00083-7

Cited by 81 publications

(63 citation statements)

References 46 publications

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“…Wang and Groopman (1999) reported that fumonisins are the only carcinogenic mycotoxins not having a direct DNA damage inducing effect, so genotoxicity is an indirect consequence of the oxidative damage caused by the toxin. On the other hand, according to Galvano et al (2002) FB 1 induced DNA damage without increased reactive oxygen species (ROS) production and may modulate some gene expression directly.…”

Section: Antioxidants and Genotoxicitymentioning

confidence: 99%

Individual and combined effects of feed artificially contaminated with with fumonisin B1 and T-2 toxin in weaned rabbits

Hafner

Szabó

D’Costa

et al. 2016

WMJ

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Co-contamination of feed and feed raw materials with two or more mycotoxins is frequently reported, however, only a few studies have investigated the combined effects of low doses of multiple mycotoxins. In the present study the individual and combined effects of 10 mg/kg fumonisin B 1 and 2 mg/kg T-2 toxin (n=12/group) were investigated in weaned rabbits. Mycotoxin contaminated feed was produced by adding fungal cultures of Fusarium verticillioides and Fusarium sporotrichioides, and fed to 40 days old rabbits during 28 days. Feed intake and body weight were measured weekly, serum biochemistry and antioxidant parameters on day 0, 14 and 28, while histopathological examination and comet assay were performed at the end of the experiment. T-2 exposure both alone and in combination resulted in 15-18% less final body weight compared to the control and FB 1 treatment. There was a significant increase in the concentration of plasma total protein, albumin, fructosamine and creatinine in the group treated with FB 1 compared to the control. The liver and the kidney of most animals treated with T-2 toxin, FB 1 and their combination showed pathological changes, occurring more frequent in animals exposed to both toxins. T-2 resulted in depletion of lymphocytes in the spleen. FB 1 and T-2 exerted synergistic effect on the antioxidant/oxidative parameters after 2 weeks of exposure, manifesting in less glutathione and glutathione peroxidase, while more malondialdehyde was produced. Both toxins caused DNA damage in the lymphocytes, which was more pronounced in the group fed T-2 toxin and T-2 combined with FB 1 , without additive or synergistic effects.

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Section: Antioxidants and Genotoxicitymentioning

confidence: 99%

Individual and combined effects of feed artificially contaminated with with fumonisin B1 and T-2 toxin in weaned rabbits

Hafner

Szabó

D’Costa

et al. 2016

WMJ

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“…Entretanto, algumas descobertas sugerem que danos celulares oxidativos podem ser devido à toxicidade e não pelo efeito celular químico direto (DRAGAN et al, 2001). Galvano et al (2002) mostraram que o tratamento com FB 1 induz danos ao DNA de fibroblastos humanos, quando as células são expostas a altas concentrações desta micotoxina e por períodos prolongados. Estes danos parecem ser de caráter apoptótico, sendo precedido de um aumento da atividade da caspase-3, assim como no nível de fragmentação da PARP [poli-ADP-(ribose)-polimerase] (GALVANO et al, 2002).…”

Section: Fumonisinas E Carcinogenicidadeunclassified

Fumonisinas: efeitos toxicológicos, mecanismo de ação e biomarcadores para avaliação da exposição

et al. 2004

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ResumoFumonisinas são toxinas produzidas por Fusarium verticillioides Sacc. Niremberg (F. moniliforme Sheldon), patógeno primário de milho, que está implicado em inúmeras doenças animais, com ênfase em leucoencefalomalácia em eqüinos, edema pulmonar em suínos e carcinomas hepáticos e renais em ratos e camundongos. Em seres humanos, os estudos epidemiológicos têm associado o consumo de milho altamente contaminado com fumonisinas ao câncer esofágico em populações da China e África do Sul. Além disso, a contaminação de milho por Fusarium spp. causa perdas econômicas consideráveis em todo o mundo. Estruturalmente, a fumonisina B 1 (FB 1 ) assemelha-se às bases esfingóides, sendo a toxicidade e carcinogenicidade associada à capacidade de inibir a ceramida sintetase, uma enzima chave na via de biossíntese dos esfingolipídeos, causando acúmulo de esfinganina e depleção de esfingolipídeos complexos importantes nas funções celulares. Devido à ocorrência global, as fumonisinas têm sido alvo de intensas investigações na tentativa de desenvolver biomarcadores capazes de avaliar a exposição à FB 1 e, estabelecer os níveis de contaminação que não causem danos à saúde humana e animal. Esta revisão apresenta os principais aspectos referentes à toxicidade de fumonisinas em animais e sistemas in vitro, aliado ao mecanismo de ação bioquímica e aos biomarcadores visando avaliação da exposição. Palavras-chave: Fumonisinas, esfingolipídios, Fusarium verticillioides, biomarcadores, milho. AbstractFumonisins, mycotoxins produced by Fusarium verticillioides Sacc. Niremberg (F. moniliforme Sheldon), the prevalent seed borne fungus of corn (Zea mays), have been associated with several animal diseases, such as equine leukoencephalomalacia, porcine pulmonary edema and hepatic and renal carcinomas in rats and mice. Epidemiological studies have linked consumption of fumonisin-contaminated corn with esophageal cancer in human populations in China and South Africa. In addition, fungal growth and further corn contamination by fumonisins cause great economic losses throughout the world. The

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“…On the another hand, incubation with 50 μmol L -1 for 6 days resulted in no change in the viability of rat primary astrocytes, but treatment in that study started after two weeks in vitro, when astrocytes were well mature and differentiated (30). Therefore, it is hard to compare in vitro results of FB 1 cytotoxicity.…”

Section: Experiments On Cell Culturesmentioning

confidence: 89%

“…Findings about FB 1 effects on the cell cycle and cell death are not consistent. Some report that FB 1 stimulates cell growth and inhibits apoptosis (29,53), and some claim the opposite (21,30). After FB 1 treatment, some observed apoptosis as well as necrosis.…”

Section: Figure 2 Structural Formula Of Sphingolipids and Sphingoid Bmentioning

confidence: 99%

Fumonisin B1: A Neurotoxic Mycotoxin / Fumonizin B1: Neurotoksični Mikotoksin

Domijan

2012

Archives of Industrial Hygiene and Toxicology

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Fumonisin B 1 (FB 1 ) is a mycotoxin produced by Fusarium spp. moulds that contaminate crop, predominantly maize, all around the world. More than 15 types of fumonisins have been indentifi ed so far, but FB 1 is the most abundant and toxicologically the most signifi cant one. FB 1 has a wide range of toxic effects, depending on animal species. In horses FB 1 causes equine leukoencephalomalacia (ELEM), in pigs pulmonary oedema and in experimental rodents nephrotoxicity and hepatotoxicity. In humans exposure to FB 1 is linked with higher incidence of primary liver cancer and oesophageal cancer, which are frequent in certain regions of the world (such as Transkei region in South Africa) where maize is staple food. The occurrence of neural tube defect in children in some countries of Central America (such as Mexico and Honduras) is connected with the consumption of FB 1 -contaminated maize-based food. However, possible involvement of FB 1 in the development of human diseases is not clear. Nevertheless, the International Agency for Research on Cancer (IARC) has classifi ed FB 1 as a possible carcinogen to humans (group 2B). FB 1 is a causative agent of ELEM, a brain disorder in equines, indicating that brain is a target organ of FB 1 toxicity. Several studies on experimental animals or on cell cultures of neural origin have established that FB 1 has a neurodegenerative potential, although the mechanism of its neurotoxicity is still vague. The aim of this article is to give an overview of available literature on FB 1 neurotoxicity and involved mechanisms, and to offer a new perspective for future studies.

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DNA damage in human fibroblasts exposed to fumonisin B1

Cited by 81 publications

References 46 publications

Individual and combined effects of feed artificially contaminated with with fumonisin B1 and T-2 toxin in weaned rabbits

Individual and combined effects of feed artificially contaminated with with fumonisin B1 and T-2 toxin in weaned rabbits

Fumonisinas: efeitos toxicológicos, mecanismo de ação e biomarcadores para avaliação da exposição

Fumonisin B1: A Neurotoxic Mycotoxin / Fumonizin B1: Neurotoksični Mikotoksin

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