Dna damage in gasoline station workers caused by occupational exposure to petrol vapour in turkey

Beceren, Ayfer; Akdemir, Nebahat; Omurtag, Gülden Zehra; Tatlıpınar, Mücteba Eşref; Şardaş, Semra

doi:10.23893/1307-2080.aps.0545

Cited by 3 publications

(6 citation statements)

References 25 publications

(29 reference statements)

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“…[11][12][13][14][15] According to existing evidence, petroleum hydrocarbons have the potential to cause deoxyribo nucleic acid (DNA) damage in gasoline-exposed individuals and exposure to petrol vapor induces genotoxic effects, confirming that the gas station workers have a high risk of cancer due to their daily occupational exposure. 17,20,21 Once gasoline is inhaled, benzene, as the main ingredient of gasoline, enters the lungs then is passed to the blood stream from which it goes to the liver, where three main phenolic metabolites of benzene are released, transient phenol and accumulated hydroquinone and catechol, in relatively high concentrations. 18,19 Benzene is a lipophilic agent, so its metabolites go directly to fatty tissues such as bone marrow where actual toxic species are generated.…”

Section: Introductionmentioning

confidence: 99%

Effect of Gasoline Exposure on Hematological Parameters of Gas Station Workers in Mekelle City, Tigray Region, Northern Ethiopia

Teklu¹,

Negash²,

Asefaw³

et al. 2021

JBM

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Background:The adverse health effects of chronic gasoline exposure may be related to impairment of the hematopoietic system with bone marrow suppression, an increased risk of blood cell morphology abnormality and developing cancer. Objective: To assess the effect of gasoline exposure on hematological parameters among gas station workers in Mekelle City, Tigray Region, Northern Ethiopia. Methods: This cross-sectional study was carried out on 43 subjects (exposed group) and 77 subjects (unexposed group) with matched age and sex. Socio-demographic characteristics and duration of exposure data were collected using a structured questionnaire and an observation checklist. Sysmex XP-300 was used for hematological analysis and stained peripheral blood smear was examined for any abnormality. Data were entered and analyzed using SPSS version 23. Results: Of exposed individuals, 28/43 (65.1%) and 49/77 (63.6%) of controls were males. The average exposure time was 5.19±4.38 years, with an average working hour of 11.74 ±1.89 hours/day. The mean RBC count (10 12 /L), HCT (%), HGB (g/dl) and platelets count (10 9 /L) of the exposed group were significantly lower (4.88±0.573, 43.29±3.71, 15.04±1.33 and 248.95±58.19) compared with controls (5.35±0.533, 44.95±3.10, 15.59±1.26 and 292.45 ±62.17) at p<0.05, respectively. The MCH (pg) (30.48±2.06 vs 29.52±1.66) and MCHC (g/ dl) (34.83±0.988 vs 34.32±0.927) were significantly higher in the exposed group compared with controls (p<0.05). HCT, RBC, HGB and platelet counts were significantly decreased with increased years of exposure (p<0.05). The peripheral blood film examination revealed basophilic stippling and macrocytosis in 9.3% of the exposed group. Conclusion: Long-term exposure to gasoline at gas stations affected RBC parameters and platelet count. A significant negative correlation was noted between duration of exposure and HGB, HCT and platelet count, warranting implementation of protective measures at gas stations.

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Section: Introductionmentioning

confidence: 99%

Effect of Gasoline Exposure on Hematological Parameters of Gas Station Workers in Mekelle City, Tigray Region, Northern Ethiopia

Teklu¹,

Negash²,

Asefaw³

et al. 2021

JBM

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“…The exposure group was divided into subgroups according to their working durations (<5, 5–20, and >20 years). Although DNA damage was significantly greater in the group working for less than 5 years and between 5 and 20 years, there was no significant increase in DNA damage in workers exposed for more than 20 years (Beceren et al, 2016). Similar to these studies, we divided individuals into two subgroups according to their duration of work (less than 7 years, and 7 years and more).…”

Section: Discussionmentioning

confidence: 86%

“…The majority of these studies have argued that this exposure would pose a future health risk for workers and might be classified as cancer susceptibility (Filho et al, 2018). As for Turkey, there are only three studies investigating the possible genotoxic effects of benzene and related chemicals in gasoline station attendants (Beceren et al, 2016; Celik and Akbaş, 2005; Celik et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…In several studies, years of employment was also observed to heighten the genotoxicity biomarkers (Beceren et al, 2016; Bindhya et al, 2010; Bukvic et al, 1998). In the aforementioned Indian study with a sample size of 27 gas station attendants/27 controls (Bindhya et al, 2010), an increase in the frequency of chromatid-type aberrations was found in gasoline station attendants with longer exposure time (more than 8 years) compared with attendants with shorter exposure (less than 8 years).…”

Section: Discussionmentioning

confidence: 99%

“…These differences include factors such as climatic conditions, working hours, and the quality and amount of fuel components, apart from the availability of full-service stations (Alyami, 2017). In addition, levels of exposure to those chemicals may vary according to the individual precautions taken, shift length, job rotation, and workplace conditions (Beceren et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of genotoxicity biomarkers in gasoline station attendants due to occupational exposure

Elkama,

Şentürk,

Karahalil

2024

Toxicol Ind Health

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Gasoline station attendants are exposed to numerous chemicals that might have genotoxic and carcinogenic potential, such as benzene in fuel vapor and particulate matter and polycyclic aromatic hydrocarbons in vehicle exhaust emission. According to IARC, benzene and diesel particulates are Group 1 human carcinogens, and gasoline has been classified as Group 2A “possibly carcinogenic to humans.” At gas stations, self-service is not implemented in Turkey; fuel-filling service is provided entirely by employees, and therefore they are exposed to those chemicals in the workplace during all working hours. Genetic monitoring of workers with occupational exposure to possible genotoxic agents allows early detection of cancer. We aimed to investigate the genotoxic damage due to exposures in gasoline station attendants in Turkey. Genotoxicity was evaluated by the Comet, chromosomal aberration, and cytokinesis-block micronucleus assays in peripheral blood lymphocytes. Gasoline station attendants ( n = 53) had higher tail length, tail intensity, and tail moment values than controls ( n = 61). In gasoline station attendants ( n = 46), the frequencies of chromatid gaps, chromosome gaps, and total aberrations were higher compared with controls ( n = 59). Increased frequencies of micronuclei and nucleoplasmic bridges were determined in gasoline station attendants ( n = 47) compared with controls ( n = 40). Factors such as age, duration of working, and smoking did not have any significant impact on genotoxic endpoints. Only exposure increased genotoxic damage in gasoline station attendants independently from demographic and clinical characteristics. Occupational exposure-related genotoxicity risk may increase in gasoline station attendants who are chronically exposed to gasoline and various chemicals in vehicle exhaust emissions.

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Chromatographic analysis of methyl hippuric acid metabolite in the urine of petroleum workers and evaluation of cytogenetic changes of epithelial cells in buccal mucosa smears

Chethan,

Gurupadayya,

Doddawad

et al. 2023

Preprint

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Purpose: Millions of workers in various occupational settings face potential exposure to hazardous substances, including a wide array of compounds found in petroleum derivatives. Among these compounds, toluene and xylene are prominent examples known for their genotoxic properties. Toluene and xylene, both belonging to the aromatic compound family, give rise to the main metabolic byproducts of hippuric acid, (o, p, m) methyl hippuric acid. Methods: HPLC approach is used to analyze the urine of petroleum workers who are exposed to toluene and xylene to assess their concentrations of the metabolites. Hippuric acid and (o, p, m) Methyl hippuric acid. The micronucleus assay of buccal mucosa smears can be used to evaluate the genotoxic effects of these exposures in petroleum workers. Occupational exposure to petroleum and its derivatives may cause DNA damage and chromosomal abnormalities in buccal mucosa cells, indicating a potential increased risk of cancer and other health effects. Results: The Method was developed for Hippuric acid and (o, p, m) Methyl hippuric acid of urinary samples of petroleum workers. The urinary HA, (o, p, m) MHA levels in cases were found to be 26.042 µg/ml with standard deviation value is 12.38. The study focused to examine the cellular alterations in exfoliated oral epithelial cells, particularly cytomorphometry and micronuclei, which were examined using PAP-stained smear to determine the existence of micronuclei. Conclusions: The urinary HA, (o, p, m) MHA levels were highly significant and the prevalence of the condition was found to be greater among petroleum workers compared to the control population. It is observed that more micro nuclei were found in buccal mucosa of petroleum workers than control subjects. Hence it is suspected to induce toxicity in petroleum workers.

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Dna damage in gasoline station workers caused by occupational exposure to petrol vapour in turkey

Cited by 3 publications

References 25 publications

Effect of Gasoline Exposure on Hematological Parameters of Gas Station Workers in Mekelle City, Tigray Region, Northern Ethiopia

Effect of Gasoline Exposure on Hematological Parameters of Gas Station Workers in Mekelle City, Tigray Region, Northern Ethiopia

Assessment of genotoxicity biomarkers in gasoline station attendants due to occupational exposure

Chromatographic analysis of methyl hippuric acid metabolite in the urine of petroleum workers and evaluation of cytogenetic changes of epithelial cells in buccal mucosa smears

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