DNA damage in bone marrow and blood cells of mice exposed to municipal sludge leachates

Tewari, A.; Dhawan, Alok; Gupta, S.K.

doi:10.1002/em.20203

Cited by 15 publications

(3 citation statements)

References 40 publications

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“…Similar reports by Alimba et al, (2006), Amahdar et al, (2009), Alabi and Bakare (2011) and Alimba et al, (2012) further reported the genotoxic and necrotic effects of leachates obtained from various dump sites. Tewari et al, (2006) reported that DNA damage can occur in bone marrow and blood cells of mice exposed to municipal sludge leachates. Histopathology profiles of the liver was also carried out in order to assess the effects of the varying concentrations of leachates samples on mice due to the significance of the liver as one of the most sensitive predictors of chemical toxicity (Tayeb et al 2010;Alimba et al 2012).…”

mentioning

confidence: 99%

Genotoxicity and histopathological assessment of raw and simulated leachates exposed to mice

Iyiola¹,

Adeyemi-Ale²,

Anifowoshe³

et al. 2017

Journal of Applied Sciences and Environmental Management

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Indiscriminate solid waste disposal to the environment without proper treatment poses a serious threat to the public health. This study was carried out to investigate genotoxicity of the raw and simulated leachates samples collected from Amilegbe municipal dump site located in Ilorin metropolis in Nigeria. Some of the physicochemical properties of both raw and simulated leachate samples were determined. Mice were exposed to 1%, 2.5%, 5%, 10% and 25% (raw leachate) and 1%, 5% and 10% concentrations (simulated leachate) respectively for 35 days. Distilled water and colchicine were used for negative and positive control respectively. Two standard genotoxicity tests namely abnormal sperm-head morphology and micronucleus (MN) assays were used to assess possible genotoxicity of the raw and simulated leachates samples. Sperm head morphology assay showed some abnormalities (folded, amorphous, banana shaped, two tails, pin tail among others) and the frequencies of the abnormalities increases was concentrationdependent. Congestion, necrosis, degeneration and appearance of vacuolation were observed in the histopathological analysis of the liver. The histopathological changes were also more pronounced with higher concentrations of raw and simulated leachates. MN and abnormal nuclei frequencies also showed statistically significant differences (p>0.05) such that MN and other nuclear abnormalities reached the maximum at the highest concentration (25% >10% >5% >2.5%>1%). This study shows that the raw and simulated leachates contain potentially genotoxic and cytotoxic substances capable of causing DNA damage. ©JASEM https://dx.doi.org/10.4314/jasem.v21i5.11

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confidence: 99%

Genotoxicity and histopathological assessment of raw and simulated leachates exposed to mice

Iyiola¹,

Adeyemi-Ale²,

Anifowoshe³

et al. 2017

Journal of Applied Sciences and Environmental Management

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“…These include lymphocytes isolated from patients' whole blood. They are exposed to contaminants from environmental matrices also as a result of accidental ingestion and are considered cells to show early signs associated with adverse health effects after exposure to the contaminants [69,70].…”

Section: The Use Of Selected Human Cell Lines In Studies Of the Basal Toxicity Genotoxicity Cytotoxicity And An Estrogenic Potential Of Lmentioning

confidence: 99%

Human Cell Culture, a Pertinent In Vitro Model to Evaluate the Toxicity of Landfill Leachate/Sewage Sludge. A Review

Jabłońska-Trypuć

2021

Environments

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Both landfill leachate and sewage sludge are complex mixtures of many potentially toxic substances in unknown and unpredictable amounts and concentrations. Both types of matrices can pose a risk to human health and the functioning of ecosystems if released into the environment. Therefore, constant monitoring of the toxicity of these mixtures is necessary. However, traditional methods of analysis of sewage sludge/landfill leachate are mainly based on physicochemical studies that do not fully reflect the effects of these mixtures on living organisms. For this purpose, research based on biological models, including mammalian, mainly human, cells is recommended and increasingly implemented. A variety of cytotoxicity tests, based on various metabolic transformations in living cells, are a very useful tool in landfill leachate/sewage sludge toxicology studies. This paper reviews the methods used in the study of the cytotoxicity of environmental matrices and the cell lines used in these studies as biological models.

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“…The results of dose-response experiments on mice tissues indicated that leachates from municipal sludge can lead to DNA damage (Tewari et al, 2006). The analysis of the municipal sludge leachates showed the presence of cadmium, chromium, copper, nickel lead, zinc and high concentrations of sulfate and nitrate.…”

Section: Wastewater and Sludge Disposalmentioning

confidence: 99%

Health Effects Associated with Wastewater Treatment, Disposal, and Reuse

Fu¹,

Ikehata²,

Buchanan³

et al. 2007

Water Environment Research

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DNA damage in bone marrow and blood cells of mice exposed to municipal sludge leachates

Cited by 15 publications

References 40 publications

Genotoxicity and histopathological assessment of raw and simulated leachates exposed to mice

Genotoxicity and histopathological assessment of raw and simulated leachates exposed to mice

Human Cell Culture, a Pertinent In Vitro Model to Evaluate the Toxicity of Landfill Leachate/Sewage Sludge. A Review

Health Effects Associated with Wastewater Treatment, Disposal, and Reuse

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