DNA damage effect of cyprodinil and thiacloprid in adult zebrafish gills

Toğay, Vehbi Atahan; Türel, Gülçin Yavuz; Çelik, Dilek Aşcı; Özgöçmen, Meltem; Tülüceoğlu, Eda Evgen; Şen, İ̇smail; Ayvaz, Yusuf

doi:10.1007/s11356-020-11668-1

Cited by 12 publications

(10 citation statements)

References 29 publications

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“…DNA damage was evidently found to increase in 0.31 ppm of cyprodinil and 0.82 and 1.64 ppm thiacloprid. Hence, the study identified cyprodinil and thiacloprid as genotoxic agents and suggests further investigation [91].…”

Section: The Potential Adverse Effects Of Neonicotinoid Insecticides On Aquatic Vertebratesmentioning

confidence: 80%

Physiological Effects of Neonicotinoid Insecticides on Non-Target Aquatic Animals—An Updated Review

Malhotra

Chen

Huang

et al. 2021

IJMS

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In this paper, we review the effects of large-scale neonicotinoid contaminations in the aquatic environment on non-target aquatic invertebrate and vertebrate species. These aquatic species are the fauna widely exposed to environmental changes and chemical accumulation in bodies of water. Neonicotinoids are insecticides that target the nicotinic type acetylcholine receptors (nAChRs) in the central nervous systems (CNS) and are considered selective neurotoxins for insects. However, studies on their physiologic impacts and interactions with non-target species are limited. In researches dedicated to exploring physiologic and toxic outcomes of neonicotinoids, studies relating to the effects on vertebrate species represent a minority case compared to invertebrate species. For aquatic species, the known effects of neonicotinoids are described in the level of organismal, behavioral, genetic and physiologic toxicities. Toxicological studies were reported based on the environment of bodies of water, temperature, salinity and several other factors. There exists a knowledge gap on the relationship between toxicity outcomes to regulatory risk valuation. It has been a general observation among studies that neonicotinoid insecticides demonstrate significant toxicity to an extensive variety of invertebrates. Comprehensive analysis of data points to a generalization that field-realistic and laboratory exposures could result in different or non-comparable results in some cases. Aquatic invertebrates perform important roles in balancing a healthy ecosystem, thus rapid screening strategies are necessary to verify physiologic and toxicological impacts. So far, much of the studies describing field tests on non-target species are inadequate and in many cases, obsolete. Considering the current literature, this review addresses important information gaps relating to the impacts of neonicotinoids on the environment and spring forward policies, avoiding adverse biological and ecological effects on a range of non-target aquatic species which might further impair the whole of the aquatic ecological web.

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Section: The Potential Adverse Effects Of Neonicotinoid Insecticides On Aquatic Vertebratesmentioning

confidence: 80%

Physiological Effects of Neonicotinoid Insecticides on Non-Target Aquatic Animals—An Updated Review

Malhotra

Chen

Huang

et al. 2021

IJMS

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“…28 The LC50 value of cyprodinil in zebrafish has been shown to range from 1050 to 4420 μg/L at multiple life stages in zebrafish. 24,29 Studies have shown that cyprodinil can result in several adverse effects, including induction of DNA damage, 30 behavioral defects, 23 estrogenic activity, 29 and developmental effects (brain defects, pericardial edema, cardiotoxicity, etc.). 23,31 Cyprodinil has also been shown to alter the expression of several genes associated with brain and interrenal tissues, 23 oxidative stress and mitochondrial functions.…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, cyprodinil is a pyrimidine amine fungicide commonly used in crops, including vegetables, fruits, and rice, to control fungal diseases by suppressing the biosynthesis of important thionic amino acids in fungi. , Cyprodinil is considered an emerging environmental contaminant due to its increasing use, high detection rate, and persistency in the environment. , Reported concentrations of cyprodinil are up to 2.898 μg/L in Chile, 0.33 μg/L in Switzerland, and 0.03 μg/L in Spain . The LC50 value of cyprodinil in zebrafish has been shown to range from 1050 to 4420 μg/L at multiple life stages in zebrafish. , Studies have shown that cyprodinil can result in several adverse effects, including induction of DNA damage, behavioral defects, estrogenic activity, and developmental effects (brain defects, pericardial edema, cardiotoxicity, etc. ). , Cyprodinil has also been shown to alter the expression of several genes associated with brain and interrenal tissues, oxidative stress and mitochondrial functions. , The mixture effect of cyprodinil with other chemicals (kresoxim-methyl) in zebrafish has been evaluated.…”

Section: Introductionmentioning

confidence: 99%

In Vitro, In Vivo, and In Silico Analysis of Pyraclostrobin and Cyprodinil and Their Mixture Reveal New Targets and Signaling Mechanisms

Kim,

Bereketoglu,

Sercinoglu

et al. 2024

Chem. Res. Toxicol.

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Pyraclostrobin and cyprodinil are broad-spectrum fungicides that are used in crops to control diseases. However, they are excessively used and, as a result, end up in the environment and threaten human health and ecosystems. Hence, knowledge of their mechanisms of action is critical to revealing their environmental fate and negative effects and regulating their use. In the present study, we conducted a comprehensive study to show the adverse effects of pyraclostrobin, cyprodinil, and their mixture using zebrafish larvae and different cell lines. Several end points were investigated, including mortality, development, gene expression, reporter assays, and molecular docking simulations. We found that both compounds and their mixture caused developmental delays and mortality in zebrafish, with a higher effect displayed by pyraclostrobin. Both compounds altered the expression of genes involved in several signaling pathways, including oxidative stress and mitochondrial function, lipid and drug metabolisms, the cell cycle, DNA damage, apoptosis, and inflammation. A noteworthy result of this study is that cyprodinil and the mixture group acted as NFκB activators, while pyraclostrobin demonstrated antagonist activity. The AHR activity was also upregulated by cyprodinil and the mixture group; however, pyraclostrobin did not show any effect. For the first time, we also demonstrated that pyraclostrobin had androgen receptor antagonist activity.

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“…One of these chemical developments is the neonicotinoid (NeN) neuro-active insecticide group that is widely employed in farming around the world. The main problem of NeNs is that they present undesirable effects over the environment and human health because of their persistence. , In particular, thiacloprid (Thia) (( Z )-[3-[(6-chloro-3-pyridinyl)methyl]-2-thiazolidinylidene]cyanamide), a cyanoimine NeN compound, was recently been identified as an agent that causes DNA damage in the adult zebrafish model organism and transcriptional changes in mitochondria-related genes in honey bees . As a result of these studies, this compound is being proposed as a genotoxic agent, where its persistence is attributed to its low absorptivity of UV light and higher better photostability in comparison to other NeNs.…”

Section: Introductionmentioning

confidence: 99%

Plasmon Chemistry on Ag Nanostars: Experimental and Theoretical Raman/SERS Study of the Pesticide Thiacloprid Bond Cleavage by the Plasmon Deactivation Effect

et al. 2023

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Silver nanoparticles (AgNPs) were synthetized and employed in surface-enhanced Raman scattering measurements to study the chemical behavior when thiacloprid (Thia) interacts with the surface of Ag nanospheres (AgNSp) and Ag nanostars (AgNSt) upon excitation of the system with a 785 nm laser. Experimental results show that the deactivation of the localized surface plasmon resonance induces structural changes in Thia. When AgNSp are used, it is possible to observe a mesomeric effect in the cyanamide moiety. On the other hand, when AgNSt are employed, it promotes the cleavage of the methylene (−CH2−) bridge in Thia to produce two molecular fragments. To support these results, theoretical calculations based on topological parameters described by the atoms in molecules theory, Laplacian of the electron density at the bond critical point (∇2ρ BCP), Laplacian bond order, and bond dissociation energies were made, confirming that the bond cleavage is centered at the −CH2– bridge in Thia.

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DNA damage effect of cyprodinil and thiacloprid in adult zebrafish gills

Cited by 12 publications

References 29 publications

Physiological Effects of Neonicotinoid Insecticides on Non-Target Aquatic Animals—An Updated Review

Physiological Effects of Neonicotinoid Insecticides on Non-Target Aquatic Animals—An Updated Review

In Vitro, In Vivo, and In Silico Analysis of Pyraclostrobin and Cyprodinil and Their Mixture Reveal New Targets and Signaling Mechanisms

Plasmon Chemistry on Ag Nanostars: Experimental and Theoretical Raman/SERS Study of the Pesticide Thiacloprid Bond Cleavage by the Plasmon Deactivation Effect

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