DNA damage and translational response during detoxification from copper exposure in a wild population of Chironomus riparius

Bernabò, Paola; Gaglio, M.; Bellamoli, Francesco; Viero, Gabriella; Lencioni, Valeria

doi:10.1016/j.chemosphere.2017.01.052

Cited by 32 publications

(13 citation statements)

References 55 publications

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“…The unknown magnitude of top-down effects and trophic cascades should be considered in future experiments. Assuming that toxicants can damage or influence DNA in a variety of ways, other approaches (e.g., gene expression analyses of genes involved in responding to chemical stress in natural populations) might be very useful to understand the molecular mechanisms of organismal response to human-derived ecosystem change (Bernabò et al, 2017;Krosch et al, 2017). Threats posed by contamination by CECs even at high altitude make it important to expand knowledge concerning heat and chemical tolerance in stenothermal insect species from freshwaters already threatened by temperature changes.…”

Section: Discussionmentioning

confidence: 99%

“…The genotoxicity of the 11 pollutants was assessed with the single-cell gel electrophoresis assay (SCGE), also known as Comet assay, to detect DNA damage in individual cells in terms of strand breaks that represent one of the major damage to DNA via oxidative stress (Valverde and Rojas, 2009). The assay was performed according to Bernabò et al (2017), without isolating individual celltypes (Lee et al, 2009;Martínez-Paz et al, 2013). Eighty-three tests were performed at sublethal concentrations of each pollutant (=LC10 estimated, LOEC, NOEC or LCx calculated where x <<50, see Statistical analyses; Tab.…”

Section: Treatment Protocolmentioning

confidence: 99%

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Response of Diamesa spp. (Diptera: Chironomidae) from Alpine streams to newly emergent contaminants and pesticides

et al. 2018

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Acute toxicity and genotoxic activity of 11 pollutants were investigated in wild populations of Diamesa cinerella and Diamesa zernyi (Diptera Chironomidae) from two alpine streams (Italian Alps). D. cinerella was collected in two sites on the non-glacial Vermigliana stream, 50 m-upstream and 5-m downstream of the Wastewater Treatment Plant (WTP) at the Tonal Pass (1799 m a.s.l.). D. zernyi was collected in the Presena glacial stream, close to the glacier snout (2685 m a.s.l.). IV-instar larvae were exposed for 24-96 h to increasing concentrations of three drugs (ibuprofen-IBU, furosemide-FUR, trimethoprim-TMP), three personal care products (triclocarban-TCC, tonalid-TON, sucralose-SUCR), and five pesticides (boscalid-BOS, captan-CAP, chlorpyrifos-CPS, metolachlor-MET, terbuthylazine-TER). The experimental concentrations were from one to several million times higher than the highest environmental concentration (EC) measured in the study sites. Two mixtures of pesticides were also prepared: MIX 1K =103 x EC of CPS, MET and TER, and MIX 10K = 104 x EC of CPS, MET and TER. Species- and site-specific responses were observed for both tests. On the basis of survival data, both species resulted very resistant to pharmaceuticals (mainly to FUR for which no effects on survival and movement or pupation were observed), and more sensitive to pesticides (mainly to CPS, MET and CAP). Genotoxicity tests (Comet assay) highlighted a WTP effect under natural conditions and a genotoxic effect for 9 of the 11 tested compounds. Overall, a clear gradient of increasing resistance in larvae from the least (PR0) to the most polluted (TP_dw) site was highlighted by both tests, ecotoxicological and of genotoxicity, as also expected according to species autecology (D. zernyi is restricted to very cold and pristine habitats). D. cinerella living downstream of the effluent accumulates a significantly higher DNA damage than the other populations, highlighting a basal physiological stress condition in nature. It is plausible that these larvae possess chemical resistance strategies to survive already under natural conditions. Diamesa spp. exhibited a higher toxic resistance than any other model species tested to date under the same pollutants, probably associable to its strong cold resistance. The results emphasised that the measured concentrations of Contaminants of Emerging Concern (CECs) and pesticides seem to be far below those required to cause acute effects. However, the effects on freshwater communities of prolonged exposure to mixture of trace CECs and pesticides remain unknown.

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Section: Discussionmentioning

confidence: 99%

Section: Treatment Protocolmentioning

confidence: 99%

Response of Diamesa spp. (Diptera: Chironomidae) from Alpine streams to newly emergent contaminants and pesticides

et al. 2018

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“…Additionally, the exposure to DEHP was shown to downregulate two hemoglobin genes in Chironomus tentans (Lee et al 2006) and also downregulate the expression of the CYP4G gene in C. riparius (Herrero et al 2017). Since the cytochrome P450 (CYP) contains heme and CYP4G is involved in detoxi cation processes (Bernabò et al 2017, Martínez-Paz et al 2012, the suppressed expression of heme-related genes in our study suggests that these genes may be affected by phthalates.…”

Section: Discussionmentioning

confidence: 79%

Mono(2-ethylhexyl) Phthalate Modulates the Lipid Accumulation and Reproductive Signaling in Daphnia Magna

Cho

Seol

Baik

et al. 2021

Preprint

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Mono(2-ethylhexyl)-phthalate (MEHP) is the primary metabolite of di(2-ethylhexyl)-phthalate (DEHP), which is widely used in industry as a plasticizer. Previous studies showed that both DEHP and MEHP have been found in not only human urine samples but also natural aquatic environments, and well documented as toxicants for reproduction and endocrine disruptors, However, the effects of MEHP exposure on aquatic organisms, including invertebrates such as Daphnia magna (D. magna), are still scarce. In the present study, the lipid alterations caused by MEHP in D. magna have been identified by analyzing lipid accumulation and nontarget metabolomics. Additionally, the expressions of reproduction were investigated. Ecotoxicologically relevant concentrations (1 to 2 mg/L) and exposure time ranges (24 h to 21 days) have been tested. MEHP resulted in no mortality to D. magna with all exposure conditions, but the number of lipid droplets increased after 96 h of exposure and enhanced reproduction of female adult daphnids was observed in the 21-day of exposure. Besides, we observed that MEHP enhanced lipid metabolism with the identification of 283 potential lipid metabolites, including glycerolipids, glycerophospholipids, and sphingolipids, following 48 h of exposure. MEHP treated group exhibited significantly higher expression of ecdysone receptor (EcR) and vitellogenin 2 (Vtg2) at 6 h and 24 h. Meanwhile at 48 h, EcR and Vtg2 were downregulated in 1 and 2 mg/L MEHP exposure. Our data show that the changes in EcR pathway with different exposure time could be associated with the lipid accumulation due to lipids increment subsequently to increased reproduction in MEHP-exposed D. magna.

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“…Besides terrestrial species, several aquatic species were assayed as well. These include aquatic midges such as Chironomus riparius [345][346][347][348][349][350], Chironomus kiiensis [351], and Chironomus tentans [352]. It is expected that the number of insect species will rise with the application of the comet assay procedure in genotoxicity, especially since these animals are of great importance for humans in terms of agriculture and ecology.…”

Section: Insectsmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay, also called single cell gel electrophoresis, is a sensitive, rapid and low-cost technique for quantifying and analysing DNA damage and repair at the level of individual cells. The assay itself can be applied on virtually any cell type derived from different organs and tissues of eukaryotic organisms. Although it is mainly used on human cells, the assay has applications also in the evaluation of DNA damage in yeast, plant and animal cells. Therefore, the purpose of this review is to give an extensive overview on the usage of the comet assay in animal models from invertebrates to vertebrates, covering both terrestrial and water biota. The comet assay is used in a variety of invertebrate species since they are regarded as interesting subjects in ecotoxicological research due to their significance in ecosystems. Hence, the first part of the review (Part 1) will discuss the application of the comet assay in invertebrates covering protozoans, platyhelminthes, planarians, cnidarians, molluscs, annelids, arthropods and echinoderms. Besides a large number of animal species, the assay is also performed on a variety of cells, which includes haemolymph, gills, digestive gland, sperm and embryo cells. The mentioned cells have been used for the evaluation of a broad spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of invertebrate models and their role from an ecotoxicological point of view will also be discussed as well as the comparison of the use of the comet assay in invertebrate and human models. Since the comet assay is still developing, its increasing potential in assessing DNA damage in animal models is crucial especially in the field of ecotoxicology and biomonitoring at the level of different species, not only humans.

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DNA damage and translational response during detoxification from copper exposure in a wild population of Chironomus riparius

Cited by 32 publications

References 55 publications

Response of Diamesa spp. (Diptera: Chironomidae) from Alpine streams to newly emergent contaminants and pesticides

Response of Diamesa spp. (Diptera: Chironomidae) from Alpine streams to newly emergent contaminants and pesticides

Mono(2-ethylhexyl) Phthalate Modulates the Lipid Accumulation and Reproductive Signaling in Daphnia Magna

The comet assay in animal models: From bugs to whales – (Part 1 Invertebrates)

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