DNA damage and rpair with age in individual human lymphocytes

Singh, Narendra P.; Danner, David B.; Tice, Raymond R.; Brant, Larry J.; Schneider, Edward L.

doi:10.1016/0921-8734(90)90018-m

Cited by 191 publications

(53 citation statements)

References 21 publications

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“…The only difference observed was significantly higher damage to DNA of leukocytes in the exposed individuals over the age of 50 years compared with control persons of the same age. This finding is in line with results of an earlier paper 39) , which reported that an age-related increase in DNA damage was observed after irradiation of human lymphocytes in vitro.…”

Section: Discussionsupporting

confidence: 83%

The Effect Occupational Exposure to Ionizing Radiation on the DNA Damage in Peripheral Blood Leukocytes of Nuclear Medicine Personnel

Dobrzyńska¹,

Pachocki²,

Gajowik³

et al. 2014

Journal of Occupational Health

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The Effect Occupational Exposure to Ionizing Radiation on the DNA Damage in Peripheral Blood Leukocytes of Nuclear Medicine Personnel: Małgorzata M. DOBRZYŃSKA, et al. Department of Radiation Hygiene and Radiobiology, National Institute of Public Health – National Institute of Hygiene, Poland— Objectives The aim of this study was estimation of DNA strand breaks in leukocytes of peripheral blood of staff in a nuclear medicine department. Methods The exposed group consisted of 46 volunteers and the control group consisted of 40 volunteers. Samples consisting of 1 ml whole blood were collected by venepuncture. DNA damage in leukocytes was detected by alkaline comet assay. Results There was no correlation between the effective dose measured by individual dosimeters and DNA damage and no differences between sexes. The mean level of damage to DNA in people exposed to ionizing radiation was significantly elevated compared with control individuals. The highest value for mean comet tail moment was noted in leukocytes of PET/CT and scintigraphy technicians (1.28 vs. 0.30 for control, p=0.013). The levels of DNA damage in leukocytes of workers in category B (effective dose may exceed 1 mSv/year) were significantly enhanced. The DNA migration of leukocytes in exposed smokers and nonsmokers was similar. In the control group the damage to DNA of leukocytes in smokers was markedly but not significantly higher compared with nonsmokers. Conclusions Occupational exposure to ionizing radiation leads to enhanced levels of reversible DNA damage in leukocytes of nuclear medicine employees. The level of DNA damage depends on the kind of work. Cigarette smoking is related to the increase in DNA damage in unexposed individuals but not in nuclear medicine workers. Radiation seems to be a stronger inducer of DNA damage than smoking. Although most of the DNA damage detected by comet assay is repaired, further improvement of radiation safety should be taken under consideration.

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Section: Discussionsupporting

confidence: 83%

The Effect Occupational Exposure to Ionizing Radiation on the DNA Damage in Peripheral Blood Leukocytes of Nuclear Medicine Personnel

Dobrzyńska¹,

Pachocki²,

Gajowik³

et al. 2014

Journal of Occupational Health

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“…Thus, apoptosis in sperm can be assumed to be a parallel pathway to DNA repair in somatic cells because both serve identical functions (namely, to ensure a zero-error transmission either during cell replication or reproduction). We have shown elsewhere an increased level of DNA damage and decreased rate of its repair with aging in somatic cells (93,94). Therefore, our current findings, that DNA damage increases with age and apoptosis decreases with age in sperm, are in accordance with our above speculation that apoptosis and DNA repair are parallel pathways.…”

Section: Discussionsupporting

confidence: 81%

Effects of age on DNA double-strand breaks and apoptosis in human sperm

Singh

Müller

Berger

2003

Fertility and Sterility

392

223

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“…However, it has been shown earlier that while the basal levels of DNA damage in the Comet assay are independent of the age of the donor, an age-dependent increase in DNA damage has been observed immediately after irradiation (Singh et al, 1990). In addition, a nearly four-fold increase in MN in cultures from 80-year-old donors was measured compared with that in the cultures from newborn donors (Fenech and Morley, 1985b), and an age dependent increase of 0.58 MN year À1 was found for a female population (Thierens et al, 2000).…”

Section: Discussionmentioning

confidence: 89%

Radiosensitivity in breast cancer assessed by the Comet and micronucleus assays

et al. 2006

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Spontaneous and radiation-induced genetic instability of peripheral blood mononuclear cells derived from unselected breast cancer (BC) patients (n ¼ 50) was examined using the single-cell gel electrophoresis (Comet) assay and a modified G2 micronucleus (MN) test. Cells from apparently healthy donors (n ¼ 16) and from cancer patients (n ¼ 9) with an adverse early skin reaction to radiotherapy (RT) served as references. Nonirradiated cells from the three tested groups exhibited similar baseline levels of DNA fragmentation assessed by the Comet assay. Likewise, the Comet analysis of in vitro irradiated (5 Gy) cells did not reveal any significant differences among the three groups with respect to the initial and residual DNA fragmentation, as well as the DNA repair kinetics. The G2 MN test showed that cells from cancer patients with an adverse skin reaction to RT displayed increased frequencies of both spontaneous and radiation-induced MN compared to healthy control or the group of unselected BC patients. Two patients from the latter group developed an increased early skin reaction to RT, which was associated with an increased initial DNA fragmentation in vitro only in one of them. Cells from the other BC patient exhibited a striking slope in the dose -response curve detected by the G2 MN test. We also found that previous RT strongly increased both spontaneous and in vitro radiation-induced MN levels, and to a lesser extent, the radiation-induced DNA damage assessed by the Comet assay. These data suggest that clinical radiation may provoke genetic instability and/or induce persistent DNA damage in normal cells of cancer patients, thus leading to increased levels of MN induction and DNA fragmentation after irradiation in vitro. Therefore, care has to be taken when blood samples collected postradiotherapeutically are used to assess the radiosensitivity of cancer patients.

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DNA damage and rpair with age in individual human lymphocytes

Cited by 191 publications

References 21 publications

The Effect Occupational Exposure to Ionizing Radiation on the DNA Damage in Peripheral Blood Leukocytes of Nuclear Medicine Personnel

The Effect Occupational Exposure to Ionizing Radiation on the DNA Damage in Peripheral Blood Leukocytes of Nuclear Medicine Personnel

Effects of age on DNA double-strand breaks and apoptosis in human sperm

Radiosensitivity in breast cancer assessed by the Comet and micronucleus assays

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