DNA damage and physiological responses in an Indian major carp Labeo rohita exposed to an antimicrobial agent triclosan

Hemalatha, Devan; Nataraj, Bojan; Rangasamy, Basuvannan; Shobana, Chellappan; Ramesh, Mathan

doi:10.1007/s10695-019-00661-2

Cited by 47 publications

(13 citation statements)

References 119 publications

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“…Sensitivity of sh to the stress of a toxicant generally varies with age, size and species (Eaton and Gilbert 2008) and early developmental stages are vulnerable than other stages (Dann and Hontela 2011). This is clearly evident from the comparison of the earlier reports for 96h LC 50 value of TCS for the embryos of Oryzias latipes (0.169 mg/L), Cyprinus carpio (0.315 mg/L), Ctenopharyngodon idella (0.116 mg/L), Cirrhinus mrigala (0.131mg/L), L. rohita (0.096mg/L) by Horie et al (2018) and Dar et al (2019), early life stages or larvae of Japanese medaka (0.6 and 0.118 mg/L) by Ishibashi et al (2004) and Horie et al (2018), respectively, ngerlings of L. rohita (0.39mg/L) by Hemalatha et al (2019) and adult sh [Lepomis macrochirus (0.37 mg/L), Pimephales promelas (0.26mg/L) and Xiphophorus helleri (1.47 mg/L)] by Orvos et al (2002) and Liang et al (2013). TCS affects excitation-contraction of skeletal muscles (Fritsch et al, 2013), this could have resulted in abnormal swimming by the hatchlings in the present study.…”

Section: Discussionmentioning

confidence: 99%

Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

Sharma

Dar

Singh

et al. 2021

Preprint

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Triclosan (TCS) used commonly in pharmaceuticals and personal care products has become the most common pollutant in water. Three days old hatchlings of an indegenous fish, Labeo rohita were given 96h exposure to an environmentally relevant (0.06mg/L) and two moderately lethal concentrations (0.067 and 0.097 mg/L) of TCS and kept for 10 days of recovery for recording transcriptomic alterations in antioxidant/detoxification (SOD, GST, CAT, GPx, GR, CYP1a and CYP3a), metabolic (LDH, ALT and AST) and neurological (AchE) genes and DNA damage. The data were subjected to Principal Component Analysis (PCA) for obtaining biomarkers for the toxicity of TCS. Hatchlings were highly sensitive to TCS (96h LC 50 = 0.126mg/L and risk quotient = 40.95), 96h exposure caused significant induction of CYP3a, AChE and ALT but suppression of all other genes. However, expression of all the genes increased significantly (except for a significant decline in ALT) after recovery. Concentration dependent increase was also observed in DNA damage [Tail Length (TL), Tail Moment (TM), Olive Tail Moment (OTM) and Percent Tail DNA (TDNA)] after 96h. The damage declined significantly over 96h values at 0.06 and 0.067 mg/L after recovery, but was still several times more than control. TCS elicited genomic alterations resulted in 5-11% mortality of exposed hatchlings during the recovery period. It is evident that hatchlings of L. rohita are a potential model and PCA shows that OTM, TL, TM, TDNA, SOD and GR (association with PC1 during exposure and recovery) are the biomarkers for the toxicity of TCS.

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Section: Discussionmentioning

confidence: 99%

Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

Sharma

Dar

Singh

et al. 2021

Preprint

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“…For the 0.22 mg/kg at the same day it was also observed significant increase (p < 0.05) for WBC, lymphocytes and neutrophils. These changings may also be related to the increased variations and certain abnormalities in the numbers of the different parameters measured, suggesting that ivermectin induced metabolic stress in the fish [39,40].…”

Section: Hematology Assaysmentioning

confidence: 98%

Safety of oral administration of high doses of ivermectin by means of biocompatible polyelectrolytes formulation

Madrid

Mathews

Patta

et al. 2021

Heliyon

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The FDA-approved drug ivermectin is applied for treatments of onchocerciasis and lymphatic filariasis. The anticancer and anti-viral activities have been demonstrated stressing possibilities for the drug repurposing and therefore new information on high dosage safety is on demand. We analyzed in vivo tissue responses for high doses of ivermectin using Corydoras fish as animal model. We made intestinal histology and hematologic assays after oral administration of ivermectin transported with polyelectrolytes formulation. Histology showed any apparent damage of intestinal tissues at 0.22-170 mg of ivermectin/kg body weight. Immunofluorescence evidenced delocalization of Myosin-Vb at enterocytes only for the higher dose. Hematology parameters showed random variations after 7 days from administration, but a later apparent recover after 14 and 21 days. The study evaluated the potential of high doses of oral administration of ivermectin formulation, which could be an alternative with benefits in high compliance therapies.

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“…The concentration dependent increase in Tail length, Tail moment, Olive Tail moment and percent Tail DNA after 96h was followed by a small decline during the recovery period but the values were still 1.5-2 folds more than control. The genotoxic effects of TCS have also been reported by Binelli et al (2009), Lin et al (2012) and Hemalatha et al (2019) in zebra mussels, earthworms and ngerlings of L. rohita, respectively. TCS induces ROS production that act as reductants or oxidants and cause deleterious effects on DNA (Gniadecki et al 2001).…”

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 96%

Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

Sharma

Dar

Singh

et al. 2021

Environ Sci Pollut Res

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Triclosan (TCS) used commonly in pharmaceuticals and personal care products has become the most common pollutant in water. Three days old hatchlings of an indegenous sh, Labeo rohita were given 96h exposure to an environmentally relevant (0.06mg/L) and two moderately lethal concentrations (0.067 and 0.097 mg/L) of TCS and kept for 10 days of recovery for recording transcriptomic alterations in antioxidant/detoxi cation (SOD, GST, CAT, GPx, GR, CYP1a and CYP3a), metabolic (LDH, ALT and AST) and neurological (AchE) genes and DNA damage. The data were subjected to Principal Component Analysis (PCA) for obtaining biomarkers for the toxicity of TCS. Hatchlings were highly sensitive to TCS (96h LC 50 = 0.126mg/L and risk quotient = 40.95), 96h exposure caused signi cant induction of CYP3a, AChE and ALT but suppression of all other genes. However, expression of all the genes increased signi cantly (except for a signi cant decline in ALT) after recovery. Concentration dependent increase was also observed in DNA damage [Tail Length (TL), Tail Moment (TM), Olive Tail Moment (OTM) and Percent Tail DNA (TDNA)] after 96h. The damage declined signi cantly over 96h values at 0.06 and 0.067 mg/L after recovery, but was still several times more than control. TCS elicited genomic alterations resulted in 5-11% mortality of exposed hatchlings during the recovery period. It is evident that hatchlings of L. rohita are a potential model and PCA shows that OTM, TL, TM, TDNA, SOD and GR (association with PC1 during exposure and recovery) are the biomarkers for the toxicity of TCS.

show abstract

DNA damage and physiological responses in an Indian major carp Labeo rohita exposed to an antimicrobial agent triclosan

Cited by 47 publications

References 119 publications

Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

Safety of oral administration of high doses of ivermectin by means of biocompatible polyelectrolytes formulation

Genomic markers for the biological responses of Triclosan stressed hatchlings of Labeo rohita

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