DNA damage and nitric oxide synthesis in experimentally infected Balb/c mice with Trypanosoma cruzi

Ribeiro, Daniel Araki; Calvi, Sueli Aparecida; Picka, Mariele M.; Persi, Eliana; Carvalho, Thaís Batista de; Caetano, Priscila K.; Nagoshi, Lidiana R.; Lima, Carlos Roberto Gonçalves de; Machado, Jussara Marcondes; Salvadori, Daisy Maria Fávero

doi:10.1016/j.exppara.2006.12.007

Cited by 34 publications

(27 citation statements)

References 37 publications

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“…When viewed under a microscope, a damaged cell has the appearance of a comet, with a head (nuclear region) and a tail that contains DNA fragments or strands migrating towards the anode (8). Previous studies have demonstrated that the comet assay is a suitable tool to investigate genotoxicity in vivo (9,10). The extent of the comet is directly related to the level of DNA damage (11,12).…”

Section: Introductionmentioning

confidence: 99%

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The influence of sleep deprivation and obesity on DNA damage in female Zucker rats

Tenório¹,

Ribeiro²,

Alvarenga³

et al. 2013

Clinics

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OBJECTIVE:The aim of this study was to evaluate overall genetic damage induced by total sleep deprivation in obese, female Zucker rats of differing ages.METHOD:Lean and obese Zucker rats at 3, 6, and 15 months old were randomly distributed into two groups for each age group: home-cage control and sleep-deprived (N = 5/group). The sleep-deprived groups were deprived sleep by gentle handling for 6 hours, whereas the home-cage control group was allowed to remain undisturbed in their home-cage. At the end of the sleep deprivation period, or after an equivalent amount of time for the home-cage control groups, the rats were brought to an adjacent room and decapitated. The blood, brain, and liver tissue were collected and stored individually to evaluate DNA damage.RESULTS:Significant genetic damage was observed only in 15-month-old rats. Genetic damage was present in the liver cells from sleep-deprived obese rats compared with lean rats in the same condition. Sleep deprivation was associated with genetic damage in brain cells regardless of obesity status. DNA damage was observed in the peripheral blood cells regardless of sleep condition or obesity status.CONCLUSION:Taken together, these results suggest that obesity was associated with genetic damage in liver cells, whereas sleep deprivation was associated with DNA damage in brain cells. These results also indicate that there is no synergistic effect of these noxious conditions on the overall level of genetic damage. In addition, the level of DNA damage was significantly higher in 15-month-old rats compared to younger rats.

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Section: Introductionmentioning

confidence: 99%

“…(13-17). For example, we have used this technique to detect DNA breakage in vivo in medium-term carcinogenesis assays and parasitic infections and following exposure to xenobiotics that are present in the environment (9,15,18). …”

Section: Introductionmentioning

confidence: 99%

The influence of sleep deprivation and obesity on DNA damage in female Zucker rats

Tenório¹,

Ribeiro²,

Alvarenga³

et al. 2013

Clinics

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“…iNOS is a potent modulator of chemokine expression which is critical to triggering the generation of the inflammatory infiltrate during T. cruzi infection [72]. DNA damage and NOS activity was seen in infected mice with T. cruzi [73]. Moreover, cytokine and NO is produced by T. brucei infection in rats [74].…”

Section: Trypanosoma Spmentioning

confidence: 99%

Involvement of nitric oxide and its up/down stream molecules in the immunity against parasitic infections

Nahrevanian

2009

Braz J Infect Dis

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Nitric oxide (NO) is a potent mediator with diverse roles in regulating cellular functions and signaling pathways. The NO synthase (NOS) enzyme family consists of three major isoforms, which convey variety of messages between cells, including signals for vasorelaxation, neurotransmission and cytotoxicity. This family of enzymes are generally classified as neuronal NOS (nNOS), endothelial NOS (eNOS) and inducible NOS (iNOS). Increased levels of NO are induced from iNOS during infection; while eNOS and nNOS may be produced at the baseline in normal conditions. An association of some key cytokines appears to be essential for NOS gene regulation in the immunity of infections. Accumulating evidence indicates that parasitic diseases are commonly associated with elevated production of NO. NO plays a role in the immunoregulation and it is implicated in the host non-specific defence in a variety of infections. Nevertheless, the functional role of NO and NOS isoforms in the immune responses of host against the majority of parasites is still highly controversial. In the present review, the role of parasitic infections will be discussed in the controversy related to the NO production and iNOS gene expression in different parasites and a variety of experimental models.

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“…Experimental studies on nourished animals inoculated with L. chagasi and Trypanosoma cruzi have demonstrated an increase in NO levels when compared to healthy animals. Our results disagree with these studies since higher NO production was observed in spontaneous culture of the healthy group (G4) compared to the inoculated nourished group (G3).…”

Section: Discussionmentioning

confidence: 99%

Nutritional status and immune response in murine experimental Jorge Lobo's disease

Barbosa

Diório

Pedrini

et al. 2015

Mycoses

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There are no studies investigating the role of nutritional status and immunity associated with Jorge Lobo's disease. The objective of this study was to evaluate the effects of protein-calorie malnutrition on the immune response of BALB/c mice inoculated with Lacazia loboi. In this study,the animals were divided into four groups: G1: inoculated with restricted diet, G2: not inoculated with restricted diet, G3: inoculated with regular diet, G4: not inoculated with regular diet. The animals of groups G1 and G2 were submitted to malnutrition for 20 days and once installed the animals were inoculated intradermally into the footpad. After 4 months, they were euthanised for the isolation of peritoneal lavage cells and removal of the footpad. The production of IL-2, IL-4, IL-10, IL-12, IFN-γ, TNF-α, H2 O2 and nitric oxide (NO) was evaluated in the peritoneal lavage cells. The footpad was evaluated regarding the size of macroscopic lesions, number of fungi and viability index. The results showed that the infection did not exert great influence on the body weight of the mice and previous malnutrition was an unfavourable factor for viability index, number of fungi, macroscopic lesion size in the footpad and production of H2 O2 , NO, IL-12, IL-10 and IFN-γ, suggesting that malnutrition significantly altered fungal activity and peritoneal cells. The results suggest considerable interaction between nutrition and immunity in Jorge Lobo's disease.

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DNA damage and nitric oxide synthesis in experimentally infected Balb/c mice with Trypanosoma cruzi

Cited by 34 publications

References 37 publications

The influence of sleep deprivation and obesity on DNA damage in female Zucker rats

The influence of sleep deprivation and obesity on DNA damage in female Zucker rats

Involvement of nitric oxide and its up/down stream molecules in the immunity against parasitic infections

Nutritional status and immune response in murine experimental Jorge Lobo's disease

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