DNA damage and micronuclei induced in rat and human kidney cells by six chemicals carcinogenic to the rat kidney

Robbiano, Luigi; Baroni, Debora; Carrozzino, Roberto; Mereto, Eugenio; Brambilla, Giovanni

doi:10.1016/j.tox.2004.06.057

Cited by 82 publications

(38 citation statements)

References 6 publications

(11 reference statements)

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“…OTA mechanisms of action are not clearly determined but the ability to generate reactive oxygen species (ROS) may explain the lipid, protein and DNA damage (Ringot et al, 2006). The test battery for evaluating OTA genotoxicity gave negative results (IARC, 1993), but some positive results are found in some in vitro and in vivo studies such as DNA breaks in mammalian cell lines (Ehrlich et al, 2002;Lebrun and Follmann, 2002;Arbillaga et al, 2007), DNA damage and micronuclei in primary cultures of human and rat kidney cells (Robbiano et al, 2004) and cytogenetic damage and DNA adducts in rats treated with OTA (Mally et al, 2005;Pfohl-Leszkowicz and Manderville, 2007).…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity of Aflatoxin B1 and Ochratoxin A after simultaneous application of the in vivo micronucleus and comet assay

Corcuera

Vettorazzi

Arbillaga

et al. 2015

Food and Chemical Toxicology

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(max. 200 words)Aflatoxin B1 (AFB1) and Ochratoxin A (OTA) are genotoxic mycotoxins that can contaminate a variety of foodstuffs, the liver and the kidney being their target organ, respectively. The micronucleus (MN) assay (bone marrow) and the comet assay (liver and kidney) were performed simultaneously in F344 rats, treated with AFB1 (0.25 mg/kg b.w.), OTA (0.5 mg/kg b.w.) or both mycotoxins. After AFB1 treatment, histopathology and biochemistry analysis showed liver necrosis, focal inflammation and an increase in Alanine Aminotransferase and Aspartate Aminotransferase. OTA alone did not cause any alteration. The acute hepatotoxic effects caused by AFB1 were less pronounced in animals treated with both mycotoxins. With regard to the MN assay, after 24h, positive results were obtained for AFB1 and negative results were obtained for OTA, although both toxins caused bone marrow toxicity. In the combined treatment, OTA reduced the toxicity and the number of MN produced by AFB1. In the comet assay, after 3h, positive results were obtained for AFB1 in the liver and for OTA in the 1 kidney. The combined treatment reduced DNA damage in the liver and had no influence in the kidney. Altogether, these results may be indicative of an antagonistic relationship regarding the genotoxicity of both mycotoxins.

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Section: Introductionmentioning

confidence: 99%

Genotoxicity of Aflatoxin B1 and Ochratoxin A after simultaneous application of the in vivo micronucleus and comet assay

Corcuera

Vettorazzi

Arbillaga

et al. 2015

Food and Chemical Toxicology

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“…Several studies have demonstrated that OTA could induce DNA damage in rat and human kidney cells, V79 Chinese hamster lung fibroblasts, rat hepatocytes, and the Chinese hamster ovary fibroblast cells (Robbiano et al, 2004;Palma et al, 2007;Cavin et al, 2009;Kamp et al, 2005a;Cosimi et al, 2009). DNA damage can cause an alteration in the genomic structure and then lead to cell death or the occurrence of mutations involved in tumorigenesis (Levy et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Ochratoxin A induces DNA damage and G2 phase arrest in human esophageal epithelium Het-1A cells <i>in vitro </i>

et al. 2015

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-Ochratoxin A (OTA), a toxin produced by several species of Aspergillus and Penicillium, is one of the most abundant food-contaminating mycotoxins. The International Agency for Research on Cancer (IARC) has classified OTA as a possible human carcinogen. Our previous study showed that there were high levels of OTA contaminations in wheat in the areas with high incidence of esophageal cancer in north China. This finding suggests that exposure to low levels of OTA may be a critical etiological factor for esophageal cancer in these areas. However, up to now, the potential biological effects of OTA on human esophageal epithelial cells have not been fully elucidated. In the present study, we explored the cytotoxicity of OTA in human esophageal epithelium immortalized cells (Het-1A). We found that OTA could induce DNA strand breaks and chromosome aberrations in Het-1A cells. OTA-induced DNA damage was followed by G2 cell cycle arrest, and down-regulation of Cdc2 and cyclinB1 contributed to the OTA-induced G2 arrest in Het-1A cells. Additionally, OTA induced apoptosis in Het-1A cells by activating caspase-3. In conclusion, our results indicated that OTA could induce DNA damage, G2 arrest and apoptosis in Het-1A cells, which may be involved in the esophageal toxicity of OTA.

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“…Heterocyclic aromatic compounds are highly ubiquitous in the environment and are known to exhibit diverse ecotoxic effects such as acute toxicity, developmental and reproductive toxicity, cytotoxicity, photo-induced toxicity, mutagenicity, and carcinogenicity (Bundy et al 2001;Barron et al 2004;Robbiano et al 2004;Brack et al 2007;Eisentraeger et al 2008).…”

Section: Toxicitymentioning

confidence: 99%

Properties, environmental fate and biodegradation of carbazole

2017

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The last two decades had witnessed extensive investigation on bacterial degradation of carbazole, an Nheterocyclic aromatic hydrocarbon. Specifically, previous studies have reported the primary importance of angular dioxygenation, a novel type of oxygenation reaction, which facilitates mineralization of carbazole to intermediates of the TCA cycle. Proteobacteria and Actinobacteria are the predominant bacterial phyla implicated in this novel mode of dioxygenation, while anthranilic acid and catechol are the signature metabolites. Several studies have elucidated the degradative genes involved, the diversity of the car gene clusters and the unique organization of the car gene clusters in marine carbazole degraders. However, there is paucity of information regarding the environmental fate as well as industrial and medical importance of carbazole and its derivatives. In this review, attempt is made to harness this information to present a comprehensive outlook that not only focuses on carbazole biodegradation pathways, but also on its environmental fate as well as medical and industrial importance of carbazole and its derivatives.

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DNA damage and micronuclei induced in rat and human kidney cells by six chemicals carcinogenic to the rat kidney

Cited by 82 publications

References 6 publications

Genotoxicity of Aflatoxin B1 and Ochratoxin A after simultaneous application of the in vivo micronucleus and comet assay

Genotoxicity of Aflatoxin B1 and Ochratoxin A after simultaneous application of the in vivo micronucleus and comet assay

Ochratoxin A induces DNA damage and G2 phase arrest in human esophageal epithelium Het-1A cells <i>in vitro </i>

Properties, environmental fate and biodegradation of carbazole

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