DNA Damage and Decrease of Cellular Oxidase Activity in Piglet Sertoli Cells Exposed to Arsanilic Acid

Abstract

ABSTRACT. The study was designed to explore the toxic effects of arsanilic acid on piglet Sertoli cells. Sertoli cells were isolated from piglet testes using a two-step enzyme digestion followed by differential plating. Piglet Sertoli cells were cultured and classified into the following five groups: group A, the control without arsanilic acid treatment; group B, cultured with 5 µM arsanilic acid; group C, cultured with 50 µM arsanilic acid; group D, cultured with 0.5 mM arsanilic acid; and group E, cultured with 5 mM arsanilic acid. We found that Sertoli cell growth was inhibited by arsanilic acid at 0.5 mM compared with the control, group A. The oxidase activity of Sertoli cells was decreased by arsanilic acid at 0.5 mM as evidenced by the observations that arsanilic acid increased MDA content but decreased the SOD and GSH-Px activities of Sertoli cells. Moreover, 50 µM of arsanilic acid was observed to cause DNA damage in Sertoli cells. The results of our study suggest that exposure of Sertoli cells to arsanilic acid leads to induction of oxidative stress and inhibition of cell growth at a high concentration, while arsanilic acid causes DNA damage in Sertoli cells at a low concentration.

Effect of arsanilic acid on anaerobic methanogenic process: Kinetics, inhibition and biotransformation analysis

“…Therefore, the methanogenic inhibition on methane production at the beginning of anaerobic digestion might be caused by arsanilic acid itself, rather than the inorganic arsenics. Zhang et al [29] has reported that arsanilic acid at a low concentration of 50 M caused DNA damage in Sertoli cells, and 0.5 mM resulted in oxidative stress and inhibition, which can also explain the inhibition of arsanilic acid to anaerobic methanogenic sludge. The inhibitory effects of arsanilic acid can be expressed by half inhibitory concentration (IC50), which is the concentration of arsanilic acid that causes a 50% reduction in the specific methanogenesis activity relative to the control.…”

Evaluation of glutathione s‐transferase as toxicity indicator for roxarsone and arsanilic acid in Eisenia fetida

“…Arsanilic acid plays an important role as a feed additive. On the other hand, it also leads to accumulation of arsenic in animals and the environment, and causes deleterious effects (Zhang et al, 2011). Meharg and Rahman (2003) showed that the inclusion of such compounds in the food chain poses an alarming threat not only to the environment but to the human health as well.…”

“…Arsanilic acid has been employed as a livestock and poultry feed additive and has become a threat to animals as well as environment and food safety because of its improper use. Excessive amounts of arsanilic acid have been used, resulting in arsenic residue and environmental pollution (Zhang et al, 2011). Increasing quantities of these kinds of synthetic chemicals in the environment require the development of biomarkers to assess and monitor their effects, which were traditionally identified only by crude effects like mortality and damage to reproduction and growth of the organisms.…”

Efeitos do caroço de algodão sobre a reprodução de bovinos

“…Sabe-se que espermatozoides anormais são formados nos animais expostos ao gossipol em decorrência de anormalidades ultra-estruturais na membrana nuclear, no retículo endoplasmático e nas mitocôndrias (Haffer, 1983;Arshami & Ruttle, 1988;Chenoweth et al, 2000;Romualdo & Klinefelter, 2002). Em células de Sertoli obtidas de testículos de suínos, esta toxina foi responsável pela inibição do crescimento e da atividade da enzima oxidase celular, além de danos ao DNA (Zhang et al, 2011). Outro efeito é a redução na expressão nuclear de receptores para andrógenos observada nas células de Leydig, de Sertoli e mióide provenientes de testículos de ratos tratados com farelo de algodão (Timurkaan et al, 2011).…”